昆虫学报 ›› 2017, Vol. 60 ›› Issue (4): 421-430.doi: 10.16380/j.kcxb.2017.04.007

• 研究论文 • 上一篇    下一篇

聚集信息素和寄主植物挥发物对光肩星天牛和星天牛的引诱作用

朱宁1, 张冬勇2, 吴利平3, 胡琴1, 樊建庭1,*   

  1.  (1. 浙江农林大学林业与生物技术学院, 生物农药高效制备技术国家地方联合工程实验室, 杭州 311300; 2. 浙江省常山油茶研究所, 浙江衢州 324200; 3. 浙江省建德市森林病虫防治检疫站, 杭州 311600)
  • 出版日期:2017-04-20 发布日期:2017-04-20

Attractiveness of aggregation pheromones and host plant volatiles to Anoplophora glabripennis and A. chinensis (Coleoptera:Cerambycidae)

ZHU Ning1, ZHANG Dong-Yong2, WU Li-Ping3, HU Qin1, FAN Jian-Ting1,*   

  1.  (1. National Joint Engineering Laboratory for Efficient Preparation of Biopesticides, School of Forestry and Biotechnology, Zhejiang A&F University, Hangzhou 311300, China; 2. Zhejiang Changshan Oil-tea Research Institute, Quzhou, Zhejiang 324200, China; 3. Jiande Forest Pest Control and Quarantine Station of Zhejiang, Hangzhou 311600, China)
  • Online:2017-04-20 Published:2017-04-20

摘要: 【目的】光肩星天牛Anoplophora glabripennis 和星天牛A. chinensis经常混合发生,危害共同的寄主,且共享相同的聚集信息素。本研究旨在评价聚集信息素和寄主植物挥发物对这两种星天牛的引诱作用。【方法】通过取食面积法,测定了两种星天牛对复叶槭Acer negundo、青皮垂柳Salix babylonica和苦楝Media azedarach 3种寄主枝条的室内取食偏好性;采用动态顶空吸附法结合气相色谱-质谱联用技术(GC-MS)分析了寄主植物挥发物成分;通过在浙江余姚和慈溪的野外诱捕试验,分析了聚集信息素和植物挥发物对两种星天牛的诱捕效果。【结果】室内选择性和非选择性取食试验结果都显示,光肩星天牛最喜食的寄主植物是复叶槭,其次是青皮垂柳,再次是苦楝;而星天牛对这3种寄主植物的选择顺序与光肩星天牛相反,最喜食苦楝,其次是青皮垂柳,再次是复叶槭。寄主植物挥发物成分中,以萜烯类和芳香族化合物居多,每种寄主挥发物都有几种特有的成分,且不同寄主植物的挥发物中多种成分之间存在显著性差异。野外林间诱捕试验结果显示,聚集信息素与苦楝混合配方MK(4-庚氧基丁醇+ 4-庚氧基丁醛+莰烯+顺-3-己烯-1-醇+罗勒烯+β-石竹烯)、聚集信息素M(4-庚氧基丁醇+4-庚氧基丁醛)、苦楝配方K(莰烯+顺-3-己烯-1-醇+罗勒烯+β-石竹烯)和柳树配方L(壬醛)都同时诱捕到了光肩星天牛和星天牛。其中,聚集信息素与苦楝混合配方MK对光肩星天牛和星天牛的诱捕效果最好,都表现出比单独使用信息素或者植物挥发物更高的诱捕效果;聚集信息素诱捕到的光肩星天牛和星天牛中雌虫比例更高,而植物挥发物诱捕到的光肩星天牛和星天牛中雄虫比例更高。【结论】本研究进一步证实了聚集信息素4-庚氧基丁醇和4-庚氧基丁醛是光肩星天牛和星天牛共享的信息素;同时,壬醛、莰烯、顺-3-己烯-1-醇、罗勒烯和β-石竹烯是光肩星天牛和星天牛共享的植物挥发物。聚集信息素和植物挥发物联合使用可以应用于光肩星天牛和星天牛的林间监测技术。

关键词: 光肩星天牛, 星天牛, 取食偏好性, 信息素, 植物挥发物, 诱捕

Abstract: 【Aim】 Anoplophora glabripennis and A. chinensis often coexist, endanger the common hosts and share the same aggregation pheromones. The aim of this study is to evaluate the attractiveness of aggregation pheromones and host plant volatiles to the two long-horned beetle species. 【Methods】 The indoor feeding preference of A. glabripennis and A. chinensis adults on twigs of three host species, Acer negundo Salix babylonica and Melia azedarach, was assayed by using feeding area method, and the components of host plant volatiles were analyzed by using dynamic headspace adsorption combined with gas chromatography-mass spectrometry (GC-MS). The trapping effects of aggregation pheromone and plant volatiles on these two species were investigated by field trapping tests in Yuyao and Cixi, Zhejiang. 【Results】 The results of indoor choice and no-choice feeding experiments showed that the most preferred host plant of A. glabripennis was A. negundo, followed by S. babylonica,and then M. azedarach, while the preference order of A. chinensis to host plants was just opposite: the most preferred host plant was M. azedarach, followed by S. babylonica, and then A. negundo. In the host plant volatiles, there were many terpenoids and aromatic compounds, and eachhost species had several unique volatile components and there were significant differences in the volatile components among different hostspecies. The results of the field trapping tests showed that the mixed formula MK (4-(n-heptyloxy)butan-1-ol+4-(n-heptyloxy)utanal+camphene+cis-3-hexen-1-ol+ocimene+β-caryophyllene) of the aggregation pheromones and M. azedarach volatiles, aggregation pheromone formula M (4-(n-heptyloxy)butan-1-ol+4-(n-heptyloxy)butanal), M. azedarach formula K (camphene+cis-3-hexen-1-ol+ocimene+β-caryophyllene) and S. babylonica formula L (nonanal) all trapped A. glabripennis and A. chinensis adults at the same time. Among them, the mixed formula MK of the aggregation pheromones and M. azedarach volatiles showed the best trapping effect on A. glabripennis and A. chinensis adults, and trapped more adults of both species than  aggregation pheromones or plant volatiles alone. The proportion of females trapped by aggregation pheromones was higher than the proportion of males, while the proportion of males trapped by plant volatiles was higher than that of females.【Conclusion】 The study further confirms that 4-(n-heptyloxy)butan-1-ol and 4-(n-heptyloxy)butanal are the common aggregation pheromones shared by A. glabripennis and A. chinensis. Meanwhile, nonanal, camphene, cis-3-hexen-1-ol, ocimene and β-caryophyllene are the common plant volatiles shared by A. glabripennis and A. chinensis. The combination of aggregation heromones and plant volatiles can be applied to the technology for monitoring A. glabripennis and A. chinensis in forests.

Key words: Anoplophora glabripennis, Anoplophora chinensis, feeding preference, pheromone, plant volatile, trapping