意大利蝗转录组及性腺发育相关基因分析

doi:10.16380/j.kcxb.2017.11.001

›› 2017, Vol. 60 ›› Issue (11): 1235-1246.doi: 10.16380/j.kcxb.2017.11.001

• 研究论文 • 下一篇

意大利蝗转录组及性腺发育相关基因分析

向敏¹, 叶小芳¹, 扈鸿霞¹, 王伟亮², 于非¹,肖宏伟², 季荣^1,*, 王晗^1,*

（1. 新疆特殊环境物种多样性应用与调控实验室, 中亚区域跨境有害生物联合控制国际研究中心, 新疆师范大学生命科学学院, 乌鲁木齐 830054; 2. 新疆玛纳斯县蝗虫鼠害预测预报防治站, 新疆玛纳斯 832200）

出版日期:2017-11-20 发布日期:2017-11-20

Analysis of the transcriptome and gonadal development related genes of Calliptamus italicus (Orthopera: Acrididae)

XIANG Min¹, YE Xiao-Fang¹, HU Hong-Xia¹, WANG Wei-Liang², YU Fei¹, XIAO Hong-Wei², JI Rong^1,*, WANG Han^1,*

(1. Key Laboratory of Special Environment Biodiversity Application and Regulation in Xinjiang, International Research Center for the Collaborative Containment of Cross-Border Pests in Central Asia, College of Life Sciences, Xinjiang Normal University, Urumqi 830054, China; 2. Manasi Station of Forecast and Control on Locusts and Rodents, Manasi, Xinjiang 832200, China)

Online:2017-11-20 Published:2017-11-20

摘要/Abstract

摘要： 【目的】意大利蝗Calliptamus italicus是新疆荒漠、半荒漠草原优势危害种类之一，其生殖相关基因信息缺乏。本研究对意大利蝗精巢和卵巢组织进行高通量转录组测序，旨在揭示意大利蝗转录组特征，并获得与性腺发育相关的基因数据。【方法】利用Illumina高通量测序平台（HiSeq/MiSeq）对意大利蝗进行了转录组测序，并进行序列分析。【结果】获得718 872条unigenes，平均长度631 bp， N50为1 186 bp。通过同源性比对，成功注释254 597条unigenes，其中，Nr数据库注释占28.87%，比例最高。Nr数据库注释的unigenes与黑褐草蚁Lasius niger的相似基因序列最多，为10.80%。与GO数据库比对发现，意大利蝗转录组unigene可分为3大类：涉及分子功能的unigenes有31 392条，涉及细胞组分的unigenes有20 586条，与生物学过程有关的unigenes有57 014条。KEGG pathways分析表明，23 666条unigenes归属于251个通路。涉及生殖系统发育的通路有卵母细胞减数分裂、胰岛素信号通路、Wnt信号通路、促性腺激素释放激素信号通路、转化生长因子β信号通路以及泛素介导的蛋白水解、黄体酮介导的卵母细胞成熟、昆虫激素生物合成等。进一步筛选得到部分与性腺发育相关的基因，包括vg, vgr, sox 21b, testisspecific serine/threonineprotein kinase, spermatogenesisassociated protein和vasa-like gene等。【结论】本研究获得了意大利蝗转录组数据及性腺发育相关基因，为进一步研究意大利蝗生殖调控机理奠定了分子基础。

关键词: 意大利蝗, 高通量测序技术, 基因注释, 卵巢, 精巢

Abstract: 【Aim】 Calliptamus italicus is a major plague pest in the arid and semi-arid grasslands of Xinjiang, with a lack of information of its reproduction related genes. The objective of this study is to reveal the characteristics of the transcriptome of C. italicus and to obtain the gonada development related genes in C. italicus through the transcriptome sequencing of its testicular and ovarian tissues. 【Methods】 The transcriptome of C. italicus was sequenced using the Illumina HiSeq/MiSeq platform and bioinformatically analyzed. 【Results】 A total of 718 872 unigenes were obtained, with a mean length of 631 bp and an N50 of 1 186 bp. Through a similarity search, 254 597 unigenes were annotated. Most unigenes (28.87%) were annotated to Nr database, and the unigenes of C. italicus had the highest similarity (10.80%) with those of Lasius niger annotated in Nr database. According to GO database, all unigenes were broadly annotated into three categories: 31 392 unigenes to be related to molecular function, 20 586 unigenes related to cellular component, and 57 014 unigenes related to biological processes. In KEGG database, a total of 23 666 unigenes were assigned to 251 metabolic pathways, of which many pathways are involved in the reproductive system development, including oocyte meiosis, insulin signaling pathway, Wnt signaling pathway, GnRH signaling pathway, TGF-β signaling pathway, ubiquitin mediated proteolysis, progesterone-mediated oocyte maturation, and insect hormone biosynthesis. By further screening and identification, gonadal development related genes of C. italicus, including genes of vg, vgr, sox 21b, testis-specific serine/threonine-protein kinase, spermatogenesis-associated protein and vasalike gene, were obtained. 【Conclusion】 This study acquired the transcriptome data and gonadal development related genes of C. italicus, providing a molecular foundation for further studying the mechanism of reproductive regulation of C. italicus.

Key words: Calliptamus italicus, high-throughput sequencing technology, gene annotation, ovary, testis

向敏, 叶小芳, 扈鸿霞, 王伟亮, 于非, 肖宏伟, 季荣, 王晗. 意大利蝗转录组及性腺发育相关基因分析[J]. , 2017, 60(11): 1235-1246.

XIANG Min, YE Xiao-Fang, HU Hong-Xia, WANG Wei-Liang, YU Fei, XIAO Hong-Wei, JI Rong, WANG Han. Analysis of the transcriptome and gonadal development related genes of Calliptamus italicus (Orthopera: Acrididae)[J]. , 2017, 60(11): 1235-1246.

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链接本文: http://www.insect.org.cn/CN/10.16380/j.kcxb.2017.11.001

http://www.insect.org.cn/CN/Y2017/V60/I11/1235

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意大利蝗转录组及性腺发育相关基因分析

Analysis of the transcriptome and gonadal development related genes of Calliptamus italicus (Orthopera: Acrididae)

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