食物中多不饱和脂肪酸在家蝇幼虫体内的富集与代谢及对其生长的影响

doi:10.16380/j.kcxb.2019.05.006

昆虫学报 ›› 2019, Vol. 62 ›› Issue (5): 578-585.doi: 10.16380/j.kcxb.2019.05.006

食物中多不饱和脂肪酸在家蝇幼虫体内的富集与代谢及对其生长的影响

杨阳^1,2, 罗坤³, 江超³, 吴建伟^1,*, 朱贵明^1,2,3,*

(1. 贵州医科大学基础医学院现代病原生物学特色重点实验室, 贵阳 550025; 2. 贵州医科大学生物与工程学院生物与医学工程重点实验室, 贵阳 550025; 3. 贵州医科大学医药生物技术工程研究中心, 贵阳 550025)

出版日期:2019-05-20 发布日期:2019-05-16

Accumulation and metabolism of polyunsaturated fatty acids from food in Musca domestica larvae and the effects on their growth

YANG Yang^1,2, LUO Kun³, JIANG Chao³, WU Jian-Wei^1,*, ZHU Gui-Ming^1,2,3,*

(1. The Key and Characteristic Laboratory of Modern Pathogen Biology, Basic Medical College, Guizhou Medical University, Guiyang 550025, China; 2. Key Laboratory of Biological and Medical Engineering, College of Biological and Engineering, Guizhou Medical University, Guiyang 550025, China; 3. Engineering Research Center of Medical Biotechnology, Guizhou Medical University, Guiyang 550025, China)

Online:2019-05-20 Published:2019-05-16

摘要/Abstract

摘要：

【目的】阐明家蝇Musca domestica幼虫对食物中各种多不饱和脂肪酸的富集能力以及代谢转化情况，并探究各种多不饱和脂肪酸对家蝇幼虫生长的影响。【方法】在基础饲料中添加不同浓度(3%, 6%和12%)的多不饱和脂肪酸(亚油酸、α-亚麻酸、花生四烯酸和二十二碳六烯酸)饲养经过脱脂传代培养的家蝇幼虫;提取家蝇幼虫的总脂肪酸，利用气相色谱仪进行检测和分析;测定统计幼虫体重,以分析多不饱和脂肪酸对家蝇幼虫生长的影响。【结果】亚油酸、α-亚麻酸和花生四烯酸在家蝇幼虫体内均能被富集，且它们的富集程度随着食物中多不饱和脂肪酸的添加浓度的升高而增加，其中亚油酸、α-亚麻酸和花生四烯酸在幼虫体内富集的最高含量(占体内总脂肪酸的比例)分别为21.93%, 16.13%和9.68%，而二十二碳六烯酸不能在家蝇幼虫体内富集，提示家蝇幼虫食物中添加的各种多不饱和脂肪酸经过代谢后并没有在其体内产生新的脂肪酸，而食物中添加的二十二碳六烯酸在家蝇幼虫体内被分解代谢后消除。饲喂α-亚麻酸及花生四烯酸后家蝇幼虫体重增长较为明显，其中6% α-亚麻酸添加组的幼虫体重显著高于对照组(取食脱脂饲料)和3%和12% α-亚麻酸添加组，3%和6%花生四烯酸添加组的幼虫体重显著高于对照组和12%花生四烯酸添加组。【结论】家蝇幼虫体内能够从食物中富集部分多不饱和脂肪酸，多不饱和脂肪酸碳链越长其富集程度越低直至不能富集，富集的多不饱和脂肪酸对家蝇幼虫生长有不同程度的影响。

关键词: 家蝇, 多不饱和脂肪酸, 气相色谱, 富集, 代谢转化, 生长发育

Abstract: 【Aim】 To clarify the accumulation and metabolic transformation of various polyunsaturated fatty acids in food by Musca domestica larvae, and to explore the effects of various polyunsaturated fatty acids on the growth of M. domestica larvae. 【Methods】 Different concentrations (3%, 6% and 12%) of polyunsaturated fatty acids (linoleic acid, alpha-linolenic acid, arachidonic acid and docosahexaenoic acid) were added to the basic diet to feed the M. domestica larvae which had been defatted and subcultured for generations, the total fatty acids of M. domestica larvae fed on the feed were extracted, detected and analyzed by gas chromatography, and the body weight of the larvae was detected to assess the effects of polyunsaturated fatty acids on the growth of M. domestia larvae. 【Results】 Linoleic acid, alpha-linolenic acid and arachidonic acid were enriched in M. domestica larvae, and their enrichment degree increased with the increase of the concentration of polyunsaturated fatty acids in food. The highest concentrations (the proportion in total fatty acids) of linoleic acid, alpha-linolenic acid and arachidonic acid enriched in larvae were 21.93%, 16.13% and 9.68%, respectively, while docosahexaenoic acid could not be enriched in M. domestica larvae, suggesting that polyunsaturated fatty acids added to the larvae of M. domestica have been metabolized without production of new fatty acids in their bodies, while docosahexaenoic acid from food is eliminated after catabolism. The body weight of M. domestica larvae was significantly increased after feeding on diets containing alpha-linolenic acid and arachidonic acid. The larval weight in the 6% alpha-linolenic acid added group was significantly higher than that in the control group (larvae feeding on defatted culture) and 3% and 12% alpha-linolenic acid added groups, and the larval weight in the 3% and 6% arachidonic acid added groups was significantly higher than that in the control group and 12% arachidonic acid added group. 【Conclusion】 M. domestica larvae can enrich some polyunsaturated fatty acids from food. The longer the carbon chain of polyunsaturated fatty acids, the lower the degree of enrichment of polyunsaturated fatty acids until they can not be enriched. The enriched polyunsaturated fatty acids have influences on the growth of M. domestica larvae in different degrees.

Key words: Musca domestica; polyunsaturated fatty acids, gas chromatography, enrichment, metabolic transformation, growth and development

杨阳, 罗坤, 江超, 吴建伟, 朱贵明. 食物中多不饱和脂肪酸在家蝇幼虫体内的富集与代谢及对其生长的影响[J]. 昆虫学报, 2019, 62(5): 578-585.

YANG Yang, LUO Kun, JIANG Chao, WU Jian-Wei, ZHU Gui-Ming. Accumulation and metabolism of polyunsaturated fatty acids from food in Musca domestica larvae and the effects on their growth[J]. Acta Entomologica Sinica, 2019, 62(5): 578-585.

[1]	刘福, 郭丽, 李青春, 张苏芳, 孔祥波, 张真, 黄范全. 杨小舟蛾雌蛾性信息素活性成分的鉴定[J]. 昆虫学报, 2019, 62(7): 814-822.
[2]	王运良, 潘忠琪, 陈可可, 陶瑞松, 苏成勇, 郝家胜, 杨群. 基于线粒体基因组控制区序列的中国冰清绢蝶遗传分化和谱系生物地理研究[J]. 昆虫学报, 2019, 62(4): 475-488.
[3]	顾冀海, 李亚静, 李文倩, 唐婷, 柳峰松. 家蝇Mdsirt1基因在细菌、高温和重金属胁迫下的表达分析[J]. , 2017, 60(12): 1394-1402.
[4]	张俊天, 熊国红, 何建国, 徐颖, 魏丽秀, 彭映辉. 三种植物精油对家蝇成虫的熏蒸活性[J]. 昆虫学报, 2016, 59(6): 641-646.
[5]	路永鹏, 顾冀海, 高一夫, 唐婷, 柳峰松, 黄大卫. 肽聚糖识别蛋白PGRP-SC在家蝇肠道免疫中的功能分析[J]. 昆虫学报, 2016, 59(3): 269-277.
[6]	邢建晓, 刘艳娟, 路永鹏, 唐婷, 柳峰松. 家蝇C-型凝集素的克隆与功能分析[J]. 昆虫学报, 2016, 59(1): 16-24.
[7]	李琳, 熊鑫, 马树杰, 马志卿, 张兴. 松油烯-4-醇光学异构体及外消旋体对家蝇的杀虫活性比较[J]. , 2015, 58(7): 761-766.
[8]	杨雪, 唐婷, 王一丽, 刘欣, 曹新茹, 倪志华, 柳峰松. 家蝇新型抗菌肽Muscin的基因克隆、表达模式及抑菌活性[J]. , 2015, 58(6): 617-624.
[9]	石庆型, 罗庆怀, 赵龙, 周正湘, 何广全, 韦卫. 与中红侧沟茧蜂生境与寄主定位相关的玉米及棉铃虫幼虫体表挥发性成分的提取与鉴定[J]. , 2015, 58(3): 244-255.
[10]	Leila MAHMOODI, Oroj VALIZADEGAN, Vahid MAHDAVI. 印度藏茴香植物精油对温室棉蚜的熏蒸毒性及其成分分析（英文）[J]. , 2015, 58(2): 147-153.
[11]	刘先福, 陈立, 李俊凯. 室内饲养的红火蚁工蚁毒腺生物碱含量动态分析[J]. , 2015, 58(1): 22-27.
[12]	李颗，李向辉，徐西林，袁哲明. 芳香羧酸衍生物驱避剂的非线性定量构效关系[J]. , 2014, 57(9): 1018-1024.
[13]	朱承节, 贺张, 陈伟, 张仕林, 胡好远. 以冷冻和新鲜家蝇蛹为寄主的蝇蛹俑小蜂实验种群生命表参数比较[J]. , 2014, 57(10): 1219-1226.
[14]	钱岑，方琦，王磊，叶恭银. 丽蝇蛹集金小蜂Pacifastin蛋白酶抑制剂基因nvpp-1和nvpp-2的功能研究[J]. , 2013, 56(8): 841-853.
[15]	卢丹，郑立，王欣欣，王凡，唐婷，柳峰松. 家蝇Denfensin-1基因的克隆、诱导表达及启动子活性分析[J]. , 2013, 56(8): 854-863.

食物中多不饱和脂肪酸在家蝇幼虫体内的富集与代谢及对其生长的影响

Accumulation and metabolism of polyunsaturated fatty acids from food in Musca domestica larvae and the effects on their growth

PDF (PC)

赞

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

Metrics

本文评价

推荐阅读 10