• 研究论文 •

### 日龄和交配状态对二化螟雄蛾内生殖器特征的影响

1. (1. 浙江大学农药与环境毒理研究所, 杭州 310058; 2. 黑龙江省植检植保站, 哈尔滨 150090; 3. 温州医科大学健康与环境生态研究所, 浙江温州 325035; 4. 四川省农业科学院植物保护研究所, 农业部西南作物有害生物综合治理重点实验室, 成都 610066)
• 出版日期:2019-07-20 发布日期:2019-07-09

### Effects of age and mating status on the characteristics of internal genital organs of male adults of the striped rice stem borer, Chilo suppressalis (Lepidoptera: Crambidae)

GUO Qian-Shuang1, WANG Chun-Rong2, SONG Xian-Dong2, FENG Bo3, CHEN Qing-Hua4, DU Yong-Jun1,*

1. (1. Institute of Pesticide and Environmental Toxicology, Zhejiang University, Hangzhou 310058, China; 2. Heilongjiang Provincial Plant Protection and Quarantine Station, Harbin 150090, China; 3. Institute of Health and Environmental Ecology, Wenzhou Medical University, Wenzhou, Zhejiang 325035, China; 4. Key Laboratory of Integrated Management of Crop Pests in Southwest China, Institute of Plant Protection, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China)
• Online:2019-07-20 Published:2019-07-09

【目的】性信息素群集诱杀应用于二化螟Chilo suppressalis测报和防治。本研究旨在明确性信息素群集诱杀二化螟的机理及诱集雄蛾的交配状态。【方法】利用行为学和生殖器解剖技术，研究不同日龄和交配状态二化螟雄蛾内生殖器特征，并将其与田间性信息素诱集获得的雄蛾进行比较。【结果】二化螟雄蛾单射精管非角质化区可分为7段；生殖器内含物主要为白色，紧实程度可以分为5级(0-4级)，存在充满和未充满生殖器两种状态。随着日龄增加，雄蛾附腺以及第3和4段非角质化区内含物等级降低，第5和7段非角质化区内含物未充满比例升高，内含物为白色的比例下降。交配后1 h之内二化螟雄蛾第5和7段非角质化区和复射精管内含物等级显著低于未交配雄蛾，而第1-4段非角质化区内含物等级显著高于未交配雄蛾。交配后24 h，所有内含物等级和未交配雄蛾无显著差异；交配后36 h，第5和7段非角质化区内含物未充满比例高于未交配雄蛾。性信息素诱集和未诱集雄蛾生殖器内含物等级无显著差异。田间性信息素诱集雄蛾附腺端半部内含物等级显著高于2-6日龄未交配二化螟，第3和4段非角质化区内含物等级显著低于刚羽化(0日龄)雄蛾。【结论】本研究结果表明，利用生殖器内含物特征可以区别交配后12 h内的已交配和未交配二化螟雄蛾，性信息素群集诱杀的可能大部分是羽化1 d的未交配雄蛾。本研究结果为二化螟雄蛾交配状态判定和蛾类害虫性信息素测报和防治的有效性提供了一定依据。

Abstract: 【Aim】 Trapping by sex pheromone has been used as an effective tool to monitor and control the striped rice stem borer, Chilo suppressalis, an important rice pest. This study aims to clarify the mechanism of sex pheromone trapping and the mating status of trapped male moths of C. suppressalis. 【Methods】 The characteristics of internal genital organs of male adults of C. suppressalis at different age (day-old) and in different mating statuses were studied with behavioral bioassay and genital anatomy and compared with those of male adults captured with sex pheromone lure in the field. 【Results】 The primary segment of single ejaculatory duct of male moth of C. suppressalis could be divided into seven segments. The genital contents in the genital duct were mostly white and could be grouped into five grades (grade 0-4) according to the compactness, with two statuses of the genital duct fully and not fully filled with contents. With the increase of the age (day-old), the grades of genital contents in accessory glands and segments 3 and 4 of ejaculatory duct decreased. The proportion of unfulfilled duct in segments 5 and 7 and the grade of contents in segments 5 and 7 increased. The grades of contents in segments 5 and 7 and in the double ejaculatory duct were significantly lower in the newly mated male moths than those in the unmated individuals of the same age, while the grades of contents in segments 1-4 of the newly mated males were significantly higher than those of the unmated individuals within 1 h after mating. At 24 h after mating, there was no significant difference in the grade of contents between mated and unmated individuals. At 36 h after mating, the proportion of unfulfilled duct in segments 5 and 7 of the mated moths was higher than that of the unmated individuals. However, the response to sex pheromones did not significantly affect the contents in the genital duct. The grades of contents in the terminal half part of accessory gland of male moths trapped by sex pheromone from the field were significantly higher than those of the unmated moths of the 2-6-day-old, while these of segments 3 and 4 of male moths trapped by sex pheromone from the field significantly lower than those of the newly emerged (0-day-old) male moths. 【Conclusion】 The results show that the characteristics of genital contents can be used to distinguish the mated and unmated individuals of C. suppressalis within 12 h after mating. Sex pheromone trapping can attract a large number of 1-day-old unmated male moths and is an effective measure for the control of C. suppressalis. Our results lay a theoretical foundation for the determination of mating status of male moths of C. suppressalis and for pest monitoring and control by pheromone trapping.