昆虫学报 ›› 2020, Vol. 63 ›› Issue (7): 825-834.doi: 10.16380/j.kcxb.2020.07.006

• 研究论文 • 上一篇    下一篇

扩头蔡白蚁肠道蛋白的鉴定

苏丽娟, 伍志伟, 高新浩, 赵鹏飞, 肖元玺, 楚君鹏, 宋安东*   

  1. (河南农业大学生命科学学院, 郑州 450002)
  • 出版日期:2020-07-20 发布日期:2020-07-29

Identification of proteins in the gut of Tsaitermes ampliceps (Isoptera: Rhinotermitidae)

SU Li-Juan, WU Zhi-Wei, GAO Xin-Hao, ZHAO Peng-Fei, XIAO Yuan-Xi, CHU Jun-Peng, SONG An-Dong*   

  1.  (College of Life Sciences, Henan Agricultural University, Zhengzhou 450002, China)
  • Online:2020-07-20 Published:2020-07-29

摘要: 【目的】本研究旨在分析比较扩头蔡白蚁Tsaitermes ampliceps工蚁前中肠和后肠及其内容物的蛋白构成和表达差异,挖掘降解木质纤维素的相关酶和蛋白。【方法】通过扩头蔡白蚁工蚁的前中肠和后肠及其内容物蛋白的双向电泳,对高表达或高差异表达的47个蛋白点进行MALDI-TOF/MS测序,并进行生物信息学分析。【结果】测序分析发现,扩头蔡白蚁肠道及其内容物蛋白中有结构蛋白13个、调节蛋白9个、白蚁代谢相关蛋白10个、微生物代谢相关蛋白7个。经PD Quest分析发现,在前中肠和后肠有11个蛋白均高表达;仅在前中肠表达的蛋白有12个,主要是白蚁代谢相关蛋白和调节蛋白;仅在后肠表达的蛋白有8个,主要是微生物代谢相关蛋白。整个肠道内参与木质纤维素降解的相关酶有5个,分别是白蚁自身分泌的内源性纤维素酶,细菌产生的内切-β-1,4-葡聚糖酶和过氧化物歧化酶以及原生动物产生的GH11。【结论】白蚁对木质纤维素食物的降解主要在前中肠,后肠对降解产物进一步降解并进行微生物生长代谢。这些降解产物和微生物菌体蛋白为白蚁的肛哺提供营养成分。

关键词:  扩头蔡白蚁, 肠道, 差异表达蛋白, 木质纤维素, 双向电泳, MALDI-TOF/MS

Abstract: 【Aim】 The aim of this study is to analyze and compare the composition and expression difference of proteins in the fore- and midgut and the hindgut including gut contents of Tsaitermes ampliceps workers, and then to excavate the enzymes and proteins that can degrade lignocellulose. 【Methods】 By two-dimensional electrophoresis of the proteins in the fore- and midgut and the hindgut including gut contents of T. ampliceps workers, 47 protein spots with high expression or high expression difference were sequenced by MALDI-TOF/MS and subjected to bioinformatics analysis. 【Results】 The sequence analysis showed that there are 13 structural proteins, 9 regulatory proteins, 10 termite metabolism-related proteins and 7 microbial metabolism-related proteins in the gut and gut content proteins of T. ampliceps. The PD Quest analysis showed that 11 proteins were highly expressed in both the fore- and midgut and the hindgut, 12 proteins mainly related to metabolism and belonging to regulatory proteins were expressed only in the fore- and midgut, and 8 proteins mainly related to microbial metabolism were expressed only in the hindgut. There are five enzymes involved in the degradation of lignocellulose in all the gut proteins, including endogenous cellulase secreted by termites themselves, endo-β-1,4-glucanase and peroxidase produced by bacteria, and GH11 produced by protozoans. 【Conclusion】 The degradation of lignocellulosic food is mainly in the fore- and midgut, and the further degradation of the degraded products and the metabolism of the products for microbial growth are mainly in the hindgut. The degraded products and bacterial proteins provide nutrients for anal feeding of termites.

Key words: Tsaitermes ampliceps, gut, differentially expressed proteins, lignocellulose, two-dimensional electrophoresis, MALDI-TOF/MS