昆虫学报 ›› 2020, Vol. 63 ›› Issue (11): 1377-1384.doi: 10.16380/j.kcxb.2020.11.010

• 研究论文 • 上一篇    下一篇

基于线粒体基因和核基因的中华虎凤蝶指名亚种地理种群遗传分化分析

向颖1,2, 董婉未3, 蒋国芳2,3,*, 洪芳2, 张佑祥1,*, 张文武4   

  1. (1. 吉首大学生物资源与环境科学学院, 湖南吉首 416000; 2. 泉州师范学院海洋与食品学院, 福建泉州 362000; 3. 南京师范大学生命科学学院, 江苏省生物多样性与生物技术重点实验室, 南京 210023; 4. 湖南乌云界国家级自然保护区管理局, 湖南常德 415700)
  • 出版日期:2020-11-20 发布日期:2020-12-08

Analysis of the genetic differentiation among geographical populations of Luehdorfia chinensis chinensis (Lepidoptera: Papilionidae) based on mitochondrial gene and nuclear genes

XIANG Ying1,2, DONG Wan-Wei3, JIANG Guo-Fang2,3,*, HONG Fang2, ZHANG You-Xiang1,*, ZHANG Wen-Wu4   

  1.  (1. College of Biology and Environmental Sciences, Jishou University, Jishou, Hunan 416000, China; 2. College of Oceanology and Food Science, Quanzhou Normal University, Quanzhou, Fujian 362000, China; 3. Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing 210023, China; 4. Wuyunjie National Nature Reserve Administration Bureau, Changde, Hunan 415700, China)
  • Online:2020-11-20 Published:2020-12-08

摘要: 【目的】探讨中华虎凤蝶指名亚种Luehdorfia chinensis chinensis地理种群的遗传分化情况。【方法】采用PCR技术对中华虎凤蝶指名亚种5个地理种群60头个体的线粒体COI基因和3个非编码核基因(ITS2, Pgi-intronTpi-intron) 片段进行扩增,利用MEGA, DnaSP和Arlequin软件对这5个地理种群的遗传变异、遗传多样性及遗传分化进行分析,并进行种群历史动态推断分析。【结果】中华虎凤蝶指名亚种地理种群线粒体基因COI和其他3个非编码核基因(ITS2, Pgi-intronTpi-intron)片段的碱基序列具有较强的AT偏好性。总群体的核苷酸多样性π=0.00686,平均核苷酸差异数K=18.289,总群体遗传多样性较高;地理种群间遗传分化程度较大(Fst=0.4910),基因交流(Nm=0.2600)不频繁。分子方差分析(AMOVA)表明,中华虎凤蝶指名亚种的遗传分化主要来自种群内部(52.79%); Mantel检验表明,地理种群间的地理距离与遗传距离是显著正相关关系(R=0.8519, P<0.05)。【结论】本研究检测的中华虎凤蝶指名亚种5个地理种群遗传多样性较高,各地理种群间具有明显的遗传分化,基因交流较少;地理距离是影响地理种群间遗传分化的重要因素之一;总群体近期并没有经历种群扩张。
关键词: 中华虎凤蝶; 地理种群; 遗传多样性; 遗传分化; 种群结构; COI; ITS2; Pgi-intron; Tpi-intron

关键词: Luehdorfia chinensis, geographical population, genetic diversity, genetic differentiation, population structure; COIITS2; Pgi-intron, Tpi-intron

Abstract: 【Aim】 To investigate the genetic differentiation of geographical populations of Luehdorfia chinensis chinensis. 【Methods】 The mitochondrial COI gene and the non-coding fragments of three nuclear genes (ITS2, Pgi-intron and Tpi-intron) of 60 individuals from five geographical populations of L. chinensis chinensis were amplified by PCR. The genetic variation, genetic diversity and genetic differentiation of the five geographical populations were analyzed by using MEGA, DnaSP and Arlequin software, and their demography was inferred. 【Results】 The base sequences of the mitochondrial gene COI and the non-coding fragments of the three nuclear genes ITS2, Pgi-intron and Tpi-intron of the geographical populations of L. chinensis chinensis have strong AT bias. The nucleotide diversity index (π) and the average number of nucleotide differences (K) of the total population were 0.00686 and 18.289, respectively, indicating a high genetic diversity of the total population. The total fixed coefficient (Fst) and the total gene flow (Nm) were 0.4910 and 0.2600, respectively, suggesting that the genetic differentiation among geographical populations is large and the gene communication infrequent. The analysis of molecular variance (AMOVA) showed that the genetic differentiation of L. chinensis chinensis mainly came within a population (52.79%). The Mantel test showed that the geographical distance between populations had a significant positive correlation with the genetic distance (R=0.8519, P<0.05). 【Conclusion】 The five geographical populations of L. chinensis chinensis tested in this study exhibit high genetic diversity, and there are obvious genetic differentiation and little gene communication among the geographical populations. Geographical distance is one of the important factors affecting genetic differentiation among the geographical populations of L. chinensis chinensis. The total population of L. chinensis chinensis has not experienced any recent population expansion.