基于形态和分子数据的山东梨园新成灾害螨柑橘全爪螨的鉴定

doi:10.16380/j.kcxb.2023.01.012

昆虫学报 ›› 2023, Vol. 66 ›› Issue (1): 92-98.doi: 10.16380/j.kcxb.2023.01.012

基于形态和分子数据的山东梨园新成灾害螨柑橘全爪螨的鉴定

陈涛^1,2, 刘永杰¹, 李波³, 门兴元⁴, 尹淑艳^{1,5, *}

(1. 山东农业大学植物保护学院, 泰安 271018; 2. 青岛西海岸现代农业示范区管委会, 青岛 266400; 3. 泰安市林业保护发展中心, 泰安 271018; 4. 山东省农业科学院植物保护研究所, 济南 250100; 5. 山东省林业有害生物防控工程技术研究中心, 泰安 271018)

出版日期:2023-01-20 发布日期:2023-03-04

Identification of Panonychus citri (Acarina: Tetranychidae), an emerging pest mite in the pear orchard in Shandong Province, East China, based on morphological and molecular data

CHEN Tao^1,2, LIU Yong-Jie¹, LI Bo³, MEN Xing-Yuan⁴, YIN Shu-Yan^1,5,*

(1. College of Plant Protection, Shandong Agricultural University, Tai′an 271018, China; 2. Management Committee of West Coast Modern Agricultural Demonstration Area, Qingdao 266400, China; 3. Tai′an Center of Forestry Protection and Development, Tai′an 271018, China; 4. Institute of Plant Protection, Shandong Academy of Agricultural Sciences, Jinan 250100, China; 5. Shandong Forestry Pest Control Engineering Technology Research Center, Tai′an 271018, China)

Online:2023-01-20 Published:2023-03-04

摘要/Abstract

摘要： 【目的】本研究旨在准确鉴定山东梨园一种新成灾害螨，为其进一步研究和有效防控奠定基础。【方法】采用体视显微镜直接观察雌成螨的形态特征，制作雄成螨的侧面观玻片标本，观察其阳茎形态特征，进行形态鉴定。从单头雌成螨中提取基因组总DNA，以螨类特异性引物扩增线粒体细胞色素氧化酶亚基Ⅰ(cytochrome oxidase subunitⅠ, COⅠ)基因及核糖体DNA第一内转录间隔区(internal transcribed spacer 1, ITS1)和第二内转录间隔区(internal transcribed spacer 2, ITS2)基因并测序，所得序列在NCBI网站上进行Blastn比对，下载与其一致性高的相关基因序列构建系统发育树和计算遗传距离，进行分子鉴定。【结果】结果表明，梨树上害螨的雌成螨背部有粗大突起，可确定为全爪螨属Panonychus害螨。雄螨阳茎特征与柑橘全爪螨Panonychus citri的很相似，其COⅠ, ITS1和ITS2基因序列均与柑橘全爪螨的相应序列具有很高的同源性(核苷酸序列一致性在99%以上)，基于COⅠ, ITS1和ITS2基因序列计算的其与柑橘全爪螨间的遗传距离均远低于其与全爪螨属其他种间的遗传距离；在基于COⅠ, ITS1和ITS2基因序列构建的系统发育树上，梨树上害螨均分别与柑橘全爪螨以很高的置信度(分别为99%, 99%和100%)聚在同一分支。【结论】形态特征结合分子数据分析可以确定山东梨园中新暴发成灾的害螨为柑橘全爪螨，说明柑橘全爪螨在中国的分布范围北移了，需引起高度重视。

关键词: 柑橘全爪螨, 梨园, 害螨, 鉴定, 阳茎, COⅠ, ITS1, ITS2

Abstract: 【Aim】 The purpose of this study is to accurately identify an emerging pest mite in the pear orchard in Shandong Province, East China, and lay the foundation for its further research and effective control. 【Methods】 The morphological characteristics of female adult mites were directly observed by stereomicroscope, and the slides of male adult mites were prepared to observe the aedeagal characteristics for morphological identification. The genomic total DNA was extracted from a single female adult mite. The mitochondrial cytochrome oxidase subunitⅠ(COⅠ) gene, and the internal transcribed spacer 1 (ITS1) gene and the internal transcribed spacer 2 (ITS2) gene of nuclear ribosomal DNA were amplified with mitespecific primers and sequenced. The obtained sequences were aligned using Blastn on NCBI. The related sequences with high identity to the obtained sequences were downloaded to construct the phylogenetic trees and calculate the genetic distances for molecular identification. 【Results】 The results showed that the female adult mite on pear trees was born with tubercles on the dorsum, which can be identified as the genus Panonychus pest mite. The characteristics of the aedeagus of the male pest mite were very similar to those of the citrus red mite, Panonychus citri. The COⅠ, ITS1 and ITS2 gene sequences of the pest mite were highly similar to the corresponding sequences of P. citri, with the nucleotide sequence identity of more than 99%. The genetic distances between the pest mite and P. citri calculated based on COⅠ, ITS1 and ITS2 gene sequences were much lower than that between it and the other species of Panonychus. On the phylogenetic trees based on COⅠ, ITS1 and ITS2 gene sequences, the pest mite on pear tree clustered in the same branch with P. citri, with very high bootstrap values (99%, 99% and 100%, respectively). 【Conclusion】 The emerging pest mite that broke out in the pear orchard in Shandong Province was determined to be P. citri based on the morphological characteristics combined with the molecular data, indicating that the distribution range of P. citri in China has moved northward, and great attention needs to be paid to this phenomenon.

Key words: Panonychus citri, pear orchard, pest mites, identification, aedeagus, COⅠ, ITS1, ITS2

陈涛, 刘永杰, 李波, 门兴元, 尹淑艳. 基于形态和分子数据的山东梨园新成灾害螨柑橘全爪螨的鉴定[J]. 昆虫学报, 2023, 66(1): 92-98.

CHEN Tao, LIU Yong-Jie, LI Bo, MEN Xing-Yuan, YIN Shu-Yan. Identification of Panonychus citri (Acarina: Tetranychidae), an emerging pest mite in the pear orchard in Shandong Province, East China, based on morphological and molecular data[J]. Acta Entomologica Sinica, 2023, 66(1): 92-98.

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基于形态和分子数据的山东梨园新成灾害螨柑橘全爪螨的鉴定

Identification of Panonychus citri (Acarina: Tetranychidae), an emerging pest mite in the pear orchard in Shandong Province, East China, based on morphological and molecular data

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