关键词:  草地贪夜蛾, 基因敲除, CRISPR/Cas9; doublesex, 翅发育

Abstract: 【Aim】 Using CRISPR/Cas9 gene editing technology to perform single-target knockout of Spodoptera frugiperda doublesex (Sfdsx), this study aims to explore the effect of Sfdsx on the wing development of male adults of S. frugiperda. 【Methods】CRISPR/Cas9 gene editing technology was used to knock out the female and male common region of Sfdsx in embryos of S. frugiperda and construct Sfdsx mutant. After pupation, the differences in the morphological characteristics of pupa and adult wings of the Sfdsx mutant were observed.【Results】 The Sfdsx mutant of S. frugiperda exhibited significant male-biased sex ratio (female∶male=0∶14), and the genital pores on the 8th-9th abdominal segments of its male pupae were severely twisted. The wing traits of the male adult mutant tended to be neutral in sex, in which，the renal patches in the center of the forewing were deformed, the black spots at the end of the wing disappeared, and the wing scales were arranged in disorder. The hind wings were deformed in wingspan, the arrangement of wing scales changed, and small black spots developed.【Conclusion】CRISPR/Cas9-mediated knockout of the common region of Sfdsx led to wing deformation of male adults of S. frugiperda. The results of our study provide an ideal gene target and theoretical basis to modulate the development of S. frugiperda through sterile insect technology (SIT).

Key words: Spodoptera frugiperda, gene knockout, CRISPR/Cas9; doublesex, wing development