关键词: 假眼小绿叶蝉, 基因组, 微卫星, 生物信息学, 高通量测序

Abstract: 【Aim】 In order to develop the molecular markers of the tea green leafhopper, Empoasca vitis, the high-throughput sequencing technologies were used in sequencing and analysis of DNA of the leafhopper. 【Methods】 Based on Illumina HiSeq sequencing technology, Illumina PE library (~400 bp) was constructed, and then the scanning of the whole genome sequences was finished using bioinformatics methods. Furthermore, MISA was used to identify the microsatellites in the genome. Ten pairs of primers were designed according to the microsatellite loci, and three-step procedure was used to screen the polymorphism of primers. 【Results】 Totally 183 194 scaffolds were examined, among which 1 545 contain microsatellites, and 1 569 microsatellites were identified in all these scaffolds. Among all 87 kinds of repeat motifs, the dominant microsatellite types were dinucleotide and trinucleotide repeats, accounting for 70.26% and 27.84% of the total SSRs, respectively, in E. vitis. CA/TG and AAT/ATTare the most frequent motifs, accounting for 33.96% and 5.86% of the total SSRs, respectively. Five primers among 10 primers designed revealed polymorphism, and 16 alleles were detected from eight individuals of E. vitis. 【Conclusion】 The results suggest that SSRs of E. vitis with polymorphism have potential for further use research. The polymorphism of SSRs may provide a molecular perspective of population divergence, dispersal mechanisms, diffusion path and influencing factors in E. vitis.

Key words: Empoasca vitis, genome, microsatellite, bioinformatics, high-throughput sequencing