绿原酸提高舞毒蛾核型多角体病毒(LdNPV)的致病力

doi:10.16380/j.kcxb.2016.05.012

摘要/Abstract

摘要： 【目的】明确绿原酸（chlorogenic acid, CA）对舞毒蛾核型多角体病毒Lymantria dispar necleopolyhydrovirus （LdNPV）致病力的影响，为舞毒蛾的防治提供参考依据。【方法】采用食料给毒法进行生物测定，测定舞毒蛾2龄幼虫对单独LdNPV及添加绿原酸的LdNPV（CA+LdNPV）的剂量及致死时间响应。【结果】CA+LdNPV 与单独LdNPV对舞毒蛾2龄幼虫的剂量及时间响应间有显著差异，二者对舞毒蛾2龄幼虫的致死中浓度（LC₅₀）分别为161.8 OBs/μL（95%的置信区间为105.6~235.3 OBs/μL）和264.4 OBs/μL（95%的置信区间为178.6~384.0 OBs/μL），前者对舞毒蛾的致病力较后者强。CA+LdNPV对舞毒蛾2龄幼虫的致死中时间（LT₅₀）较LdNPV 的短，当LdNPV浓度为590 OBs/μL，CA+LdNPV及LdNPV对舞毒蛾2龄幼虫的LT₅₀分别为9.9 d和12.3 d; 当LdNPV浓度为5 900 OBs/μL时，则LT₅₀分别为6.9 d和8.0 d。绿原酸降低了LdNPV对舞毒蛾幼虫的致死中浓度，缩短了致死中时间。【结论】绿原酸可提高LdNPV对舞毒蛾2龄幼虫的致病力，其机理有待进一步研究。

关键词: 舞毒蛾, 核型多角体病毒, 绿原酸, 致病力, 致死中浓度, 致死中时间

Abstract: 【Aim】 To identify the effects of chlorogenic acid (CA) on the virulence of Lymantria dispar necleopolyhydrovirus (LdNPV) so as to provide information for the prevention and control of the gypsy moth, L. dispar. 【Methods】 CA+LdNPV and LdNPV were bioassayed using diet plus method, to determine the dose and time response of the 2nd instar larvae of L. dispar. 【Results】 CA+LdNPV and LdNPV differed significantly in dose and time response when they were tested against the L. dispar larvae. The median lethal concentration (LC₅₀) value of CA+LdNPV to the 2nd instar larvae was 161.8 OBs/μL (the 95% confidence limit was 105.6-235.3 OBs/μL), and that of LdNPV was 264.4 OBs/μL (the 95% confidence limit was 178.6-384.0 OBs/μL). CA+LdNPV was more virulent than LdNPV against L. dispar larvae. The median lethal time (LT₅₀) value of CA+LdNPV was shorter than that of LdNPV. The LT₅₀values of CA+LdNPV and LdNPV were 9.9 and 12.3 days, respectively, at the concentration of 590 OBs/μL of LdNPV, and 6.9 and 8.0 days, respectively, at the concentration of 5 900 OBs/μL. The CA lowered the LC₅₀ value and shortened the LT₅₀ value of LdNPV against the 2nd instar larvae of L. dispar. 【Conclusion】 Chlorogenic acid enhances the virulence of LdNPV against the 2nd instar larvae of L. dispar, and its action mechanism needs further study.

Key words: Lymantria dispar, LdNPV, chlorogenic acid, virulence, median lethal concentration, median lethal time

刘海晶, 段立清, 李海平, 冯淑军, 张波波. 绿原酸提高舞毒蛾核型多角体病毒(LdNPV)的致病力[J]. 昆虫学报, 2016, 59(5): 568-572.

LIU Hai-Jing, DUAN Li-Qing, LI Hai-Ping, FENG Shu-Jun, ZHANG Bo-Bo. Chlorogenic acid enhances the virulence of Lymantria dispar necleopolyhydrovirus (LdNPV)[J]. Acta Entomologica Sinica, 2016, 59(5): 568-572.

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http://www.insect.org.cn/CN/Y2016/V59/I5/568

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绿原酸提高舞毒蛾核型多角体病毒(LdNPV)的致病力

Chlorogenic acid enhances the virulence of Lymantria dispar necleopolyhydrovirus (LdNPV)

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