›› 2017, Vol. 60 ›› Issue (6): 650-658.doi: 10.16380/j.kcxb.2017.06.005

• 研究论文 • 上一篇    下一篇


李国平, 封洪强*, 黄博, 金银利, 田彩红, 黄建荣, 邱峰   

  1. (河南省农业科学院植物保护研究所, 河南省农作物病虫害防治重点实验室, 农业部华北南部作物有害生物综合治理重点实验室, 河南省作物保护国际联合实验室, 河南省生物农药工程研究中心, 郑州 450002)
  • 出版日期:2017-06-20 发布日期:2017-06-20

Assessment of toxicities of sixteen insecticides to the plant bug, Apolygus lucorum (Hemiptera: Miridae) by glass-vial and artificial diet bioassays (In English)

LI Guo-Ping, FENG Hong-Qiang*, HUANG Bo, JIN Yin-Li, TIAN Cai-Hong, HUANG Jian-Rong, QIU Feng   

  1. (Henan Key Laboratory of Crop Pest Control, Key Laboratory of Integrated Pest Management on Crops in Southern  Region of North China, International Joint Research Laboratory for Crop Protection of Henan, Biological Pesticides  Engineering Research Center of Henan Province, Institute of Plant Protection,
    Henan Academy of Agricultural Sciences, Zhengzhou 450002, China)
  • Online:2017-06-20 Published:2017-06-20

摘要: 【目的】绿盲蝽Apolygus lucorum (Meyer-Dür)是我国Bt棉田内一类重要的刺吸性害虫。有机磷类和菊酯类杀虫剂因其对绿盲蝽具有较高的触杀毒力,是当前Bt棉田内广泛应用的杀虫剂,而新烟碱类杀虫剂因其较低的触杀毒力而没有被推荐使用。但在田间试验中,用新烟碱类杀虫剂处理棉种能够有效减少苗期至开花期绿盲蝽的危害。因此,亟待需要一种准确的毒力测定方法,以重新评价新烟碱类杀虫剂对绿盲蝽的毒力。【方法】本研究在成功研制绿盲蝽人工饲料的基础上,应用瓶膜法和人工饲料混合法两种毒力测定方法测定比较了5类16种杀虫剂对绿盲蝽3龄若虫和成虫的毒力。【结果】应用瓶膜法,新烟碱类杀虫剂对绿盲蝽成虫和若虫的LC50值在337.97~496.03 μg/mL范围内,显著高于其他类型的杀虫剂(LC50值范围在0.28~207.26 μg/mL)。然而,应用人工饲料混合法,新烟碱类杀虫剂对绿盲蝽成虫和若虫的LC50值在0.01~1.08 μg/g,与其他类杀虫剂LC50值相等甚至低于其他类杀虫剂。【结论】结果表明新烟碱类杀虫剂对绿盲蝽的胃毒毒力显著强于它的触杀毒力,可用于绿盲蝽的田间防治。推荐人工饲料混合法作为监测绿盲蝽对新烟碱类杀虫剂抗性发展的一个替代方法。

关键词: 绿盲蝽, 新烟碱类杀虫剂, 毒力, 瓶膜法, 人工饲料法

Abstract: 【Aim】 Apolygus lucorum (Meyer-Dür) (Hemiptera: Miridae) is a serious piercing sucking pest of Bt cotton in China. Currently, spraying organophosphates and pyrethroids has been widely accepted for A. lucorum management, while spraying neonicotinoids is not recommended due to their low contact toxicity. However, neonicotinoid insecticidal seed treatments effectively protect cotton from A. lucorum infestation until blooming stage. Therefore, an appropriate bioassay method needs to be developed to re-evaluate the toxicity of these insecticides to A. lucorum. 【Methods】 Two different types of bioassays, i.e., glass-vial and artificial diet toxicity bioassay, were used to assess the toxicities of 16 insecticides belonging to five chemical categories to the 3rd instar nymphs and adults of A. lucorum. 【Results】 The LC50 values of neonicotinoid insecticides to the adults and nymphs of A. lucorum ranged from 337.97 to 496.03 μg/mL in the glassvial toxicity test, which were significantly higher than those of other classes of insecticides (0.28-207.26 μg/mL). However, the LC50 values of the neonicotinoids to both adults and nymphs ranged from 0.01-1.08 μg/g in the artificial diet bioassay, which were equal to or lower than those of other classes of insecticides. 【Conclusion】 The results indicated that the ingestion toxicity of neonicotinoids is stronger than the contact toxicity to A. lucorum and these insecticides should be recommended to fight against this pest. The artificial diet test can be used as an alternative resistance monitoring method for neonicotinoids in the future.

Key words: Apolygus lucorum; neonicotinoids, toxicity, glassvial bioassay, artificial diet bioassay