不同龄期移虫发育的蜂王卵巢蛋白质组学分析(英文)

doi:10.16380/j.kcxb.2017.07.004

›› 2017, Vol. 60 ›› Issue (7): 760-771.doi: 10.16380/j.kcxb.2017.07.004

不同龄期移虫发育的蜂王卵巢蛋白质组学分析(英文)

庞倩¹, 沈芳², 王康¹, 张文文¹, 殷玲³, 吉挺^1,*

（1. 扬州大学动物科学与技术学院, 江苏扬州 225009; 2. 安徽省阜阳畜牧局, 安徽阜阳 236000; 3. 江苏农牧科技职业学院, 江苏泰州 225300）

出版日期:2017-07-20 发布日期:2017-07-20

Proteomic analysis of ovaries of queen bees (Apis mellifera) developed from larvae grafted at different instars (In English)

PANG Qian¹, SHEN Fang², WANG Kang¹, ZHANG Wen-Wen¹, YIN Ling³, JI Ting^1,*

(1. College of Animal Science and Technology, Yangzhou University, Yangzhou, Jiangsu 225009, China; 2. Fuyang Animal Husbandry and Veterinary Burean, Fuyang, Anhui 236000, China; 3. Jiangsu AgriAnimal Husbandry Vocational College, Taizhou, Jiangsu 225300, China)

Online:2017-07-20 Published:2017-07-20

摘要/Abstract

摘要： 【目的】探讨移虫龄期对西方蜜蜂Apis mellifera蜂王卵巢发育的分子调控机制。【方法】运用相对和绝对定量同位素标记(iTRAQ)技术对不同龄期移虫培育的蜂王卵巢组织蛋白质进行定量分析，筛选差异表达蛋白。利用蛋白质免疫印迹法对结果进行验证。【结果】iTRAQ定量分析共鉴定到蜂王卵巢组织蛋白质二级图谱452 966个，最终获得3 642个蛋白。GO（Gene Ontology）富集结果表明，不同龄期移虫发育的蜂王卵巢组织差异表达蛋白主要富集在细胞代谢、分裂以及蛋白质合成。Pathway富集分析表明，1龄幼虫期移虫和2龄幼虫期移虫发育的蜂王卵巢组织差异表达蛋白主要富集在碳水化合物代谢、脂代谢和外源降解类群；1龄幼虫期移虫和3龄幼虫期移虫发育蜂王卵巢组织差异蛋白主要富集在有机体发育通路、核糖体通路和溶酶体代谢。与此同时，两个差异表达蛋白即储存蛋白Hexamerin110和Hexamerin70b的蛋白免疫印迹检测结果表明，随着移虫龄期的增加，Hexamerin110和Hexamerin70b的表达量均呈现降低的趋势。【结论】对不同龄期移虫的蜂王之间差异表达蛋白进行鉴定，为进一步研究蜂王生殖发育及级型分化的调控机制提供理论依据。

关键词: 西方蜜蜂, 移虫, 龄期, 卵巢, 蜂王, 蛋白质组学, 差异表达蛋白

Abstract: 【Aim】 To explore the molecular regulation mechanism of grafted larvae on ovarian development of queens in the Western honey bee, Apis mellifera. 【Methods】 The isobaric tags for relative and absolute quantitation (iTRAQ) method was used to quantify the proteins in the ovaries of queens reared from the larvae grafted at different instars and screen the differentially expressed proteins (DEPs). The results were validated by Western blotting. 【Results】 A total of 452 966 spectra were obtained and 3 642 proteins were identified from ovaries of A. mellifera queens. Gene Ontology (GO) analysis revealed that the DEPs in the ovaries of queens reared from the larvae grafted at different instars are associated with cell metabolism, cell division, and protein biosynthesis. The DEPs in the ovaries of queens reared from the larvae grafted between the 1st and 2nd instars are enriched in such pathways as carbohydrate metabolism, lipid metabolism, and exogenous degradation, while those reared from the larvae grafted between the 1st and 3rd instars mainly enriched in developmental pathways, ribosome pathways, and lysosome metabolism. The Western blotting results of the two differentially expressed storage proteins hexamerin 110 and hexamerin 70b showed that their expression levels decreased with the grafted larval instar. 【Conclusion】 The identification of differentially expressed proteins in the ovaries of A. mellifera queens reared from the larvae grafted at different instars provides a foundation for the regulatory mechanism of queen reproductive development and caste differentiation.

Key words: Apis mellifera; grafted larva, instar, ovary, queen, proteomics, differentially expressed proteins

庞倩, 沈芳, 王康, 张文文, 殷玲, 吉挺. 不同龄期移虫发育的蜂王卵巢蛋白质组学分析(英文)[J]. , 2017, 60(7): 760-771.

PANG Qian, SHEN Fang, WANG Kang, ZHANG Wen-Wen, YIN Ling, JI Ting. Proteomic analysis of ovaries of queen bees (Apis mellifera) developed from larvae grafted at different instars (In English)[J]. , 2017, 60(7): 760-771.

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链接本文: http://www.insect.org.cn/CN/10.16380/j.kcxb.2017.07.004

http://www.insect.org.cn/CN/Y2017/V60/I7/760

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不同龄期移虫发育的蜂王卵巢蛋白质组学分析(英文)

Proteomic analysis of ovaries of queen bees (Apis mellifera) developed from larvae grafted at different instars (In English)

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