樟脑罗勒生物活性提取物对储藏豇豆和大豆中四纹豆象的影响(英文)

doi:10.16380/j.kcxb.2017.12.008

›› 2017, Vol. 60 ›› Issue (12): 1420-1429.doi: 10.16380/j.kcxb.2017.12.008

樟脑罗勒生物活性提取物对储藏豇豆和大豆中四纹豆象的影响(英文)

Daniel KOSINI^1,*, Elias Nchiwan NUKENINE², Clément SAIDOU³, Alembert Tiabou TCHINDA⁴, Gabriel Agbor AGBOR⁴

(1. School of Health Sciences, Catholic University Institute of Saint Jérôme, Douala, Cameroon; 2. Department of Biological Sciences, Faculty of Science, University of Ngaoundere, Cameroon; 3. Department of Chemical Engineering and Environment, UIT of the University of Ngaoundere, Cameroon; 4. Medical Research Institute and Studies of Medicinal Plants (IMPM), Yaoundé, Cameroon)

出版日期:2017-12-20 发布日期:2017-12-20

Effects of bioactive extracts of Ocimum canum (Lamiaceae) on Callosobruchus maculatus (Coleoptera: Chrysomelidae) in stored cowpea and soybean (In English)

Daniel KOSINI^1,*, Elias Nchiwan NUKENINE², Clément SAIDOU³, Alembert Tiabou TCHINDA⁴, Gabriel Agbor AGBOR⁴

(1. School of Health Sciences, Catholic University Institute of Saint Jérôme, Douala, Cameroon; 2. Department of Biological Sciences, Faculty of Science, University of Ngaoundere, Cameroon; 3. Department of Chemical Engineering and Environment, UIT of the University of Ngaoundere, Cameroon; 4. Medical Research Institute and Studies of Medicinal Plants (IMPM), Yaoundé, Cameroon)

Online:2017-12-20 Published:2017-12-20

摘要/Abstract

摘要： 【目的】本研究旨在评价樟脑罗勒Ocimum canum Sims (唇形科)叶的正己烷、丙酮和甲醇提取物对四纹豆象Callosobruchus maculatus (F.)的杀虫、保护和驱避潜力。【方法】用剂量为1和5 g/kg豆粒的樟脑罗勒提取物包被处理豇豆和大豆豆粒，并以相同剂量的市售苦楝籽油进行包被处理作为参照。7 内每隔1 d检查处理豆粒中四纹豆象成虫的死亡率。在四纹豆象的各个未成熟期，通过处理新产卵和幼虫，进行杀卵毒性和杀幼虫毒性测试。贮存4个月后评价豆粒受损情况和重量损失。应用线型嗅觉仪，通过选择试验测定驱避效果。【结果】提取物对四纹豆象成虫的毒性与市售苦楝籽油相同，在处理的豇豆中，提取物比市售的苦楝籽油毒性更强。5 g/kg豆粒提取物这一剂量处理7 d内，正己烷、丙酮和甲醇提取物分别引起54.2%±3.9%, 62.9%±4.8%和60.%±4.5%的成虫死亡。此外，与测试的标准杀虫剂相比，提取物激发这两种豆粒产生更强的驱避效果。这一产物对卵的毒性最强，对1和2龄幼虫的毒性比对其他发育阶段豆象的毒性强。正己烷提取物对2龄幼虫的毒性最强（致死率为81.0%±5.3%），而丙酮提取物和甲醇提取物对1龄幼虫的毒性最强，致死率分别为92.5%±7.5%和62.0%±15.7%。丙酮提取物对1和2龄幼虫的毒性与测试的标准杀虫剂相似，对蛹的毒性强于测试的标准杀虫剂。贮存4个月后，豇豆受损和重量损失分别减轻3倍和2倍多。【结论】本研究结果表明，樟脑罗勒提取物对四纹豆象具有潜在的驱避和杀虫活性，由于容易获得，因此强烈建议将该植物用于喀麦隆的害虫治理策略中。

关键词: 四纹豆象, 樟脑罗勒, 豆类, 毒性, 驱避性

Abstract: 【Aim】 The present study was carried out to evaluate the insecticidal, protectant and repellent potentials of hexane, acetone and methanol extracts of leaves of Ocimum canum Sims (Lamiaceae) against Callosobruchus maculatus (F.) (Coleoptera: Chrysomelidae). 【Methods】 Cowpea and soybean were coated with extracts from O.canum at dosages of 1 and 5 g/kg grains and commercial neem seed oil (NSO) as a reference at the same dosages. Adult mortality of C. maculatus was assessed during a period of seven days, one day interval. Ovicidal and larvicidal toxicity was tested by treating freshly laid eggs and larvae at different immature stages of C. maculatus. Grain damage and weight loss were assessed after a storage period of four months. Repellency effects were detected in choice test using a linear olfactometer. 【Results】 Extracts had the same toxicity to C. maculatus adults and proved to be more toxic than the commercial NSO in treated cowpea. Hexane, acetone and methanol extracts caused 54.2%±3.9%, 62.9%±4.8% and 60.3%±4.5% adult mortality, respectively,within seven days of exposure to the dosage of 5 g/kg cowpea grain. Moreover, extracts evoked stronger repellency effects compared with the tested standard insecticide, irrespective of grain species. The products were the most toxic to eggs, and more toxic to 1st and 2nd instar larvae than to other developmental stages. Hexane extract was the most effective against the 2nd instar larvae (81.0%±5.3% mortality), while acetone extract (92.5%±7.5% mortality) and methanol extract (62.0%±15.7% mortality) were the most effective against the 1st instar larvae. Acetone extract had a similar toxicity with the tested standard insecticide against the 1st and 2nd instar larvae and was superior against the pupae. Cowpea grain damage and weight loss were reduced by more than three- and two-fold, respectively, after four months of storage. 【Conclusion】 The outcome of this study indicates the potential of extracts from O. canum as repellent and insecticide against C. maculatus, and because of its availability the plant is strongly recommended to be used in pest management strategies in Cameroon.

Key words: Callosobruchus maculatus, Ocimum canum, pulse, toxicity, repellency

Daniel KOSINI, Elias Nchiwan NUKENINE, Clément SAIDOU, Alembert Tiabou TCHINDA, Gabriel Agbor AGBOR. 樟脑罗勒生物活性提取物对储藏豇豆和大豆中四纹豆象的影响(英文)[J]. , 2017, 60(12): 1420-1429.

Daniel KOSINI, Elias Nchiwan NUKENINE, Clément SAIDOU, Alembert Tiabou TCHINDA, Gabriel Agbor AGBOR. Effects of bioactive extracts of Ocimum canum (Lamiaceae) on Callosobruchus maculatus (Coleoptera: Chrysomelidae) in stored cowpea and soybean (In English)[J]. , 2017, 60(12): 1420-1429.

0
/ / 推荐

导出引用管理器 EndNote|Reference Manager|ProCite|BibTeX|RefWorks

链接本文: http://www.insect.org.cn/CN/10.16380/j.kcxb.2017.12.008

http://www.insect.org.cn/CN/Y2017/V60/I12/1420

[1]	石敏, 李冠楠, 郑茜, 刘凤丹, 赵珊, 蔡苗, 朱勇. 乐果对家蚕生殖腺及氧化应激反应的影响[J]. 昆虫学报, 2019, 62(10): 1186-1196.
[2]	袁锐, 李丽莉, 李超, 夏小菊, 郑礼, 于毅, 门兴元. 六种新烟碱类杀虫剂对凹唇壁蜂的毒性及风险评估[J]. , 2018, 61(8): 950-956.
[3]	Fahimeh HAMIDI, Ali MEHRVAR, Naser EIVAZIAN KARY, Hassan VALIZADEH. 百里香、欧芹和熏衣草水苏提取物对四纹豆象成虫的生物活性(英文)[J]. , 2018, 61(3): 331-339.
[4]	刘琳, 张欧, 马树杰, 马志卿, 张兴. 雷公藤吉碱对粘虫成虫飞行肌结构的影响[J]. , 2017, 60(7): 782-789.
[5]	周浩, 邵莒南, 翟一凡, 吴光安, 陈浩, 门兴元, 于毅, 郑礼. 氟啶虫胺腈、乙基多杀菌素及其混剂对地熊蜂的毒性及风险评估[J]. , 2017, 60(7): 809-816.
[6]	郭成林, 覃柳燕, 万树青, 马跃峰, 覃建林, 马永林. 黄皮种子提取物对斜纹夜蛾幼虫的杀虫活性及有效成分鉴定[J]. , 2016, 59(8): 839-845.
[7]	张俊天, 熊国红, 何建国, 徐颖, 魏丽秀, 彭映辉. 三种植物精油对家蝇成虫的熏蒸活性[J]. , 2016, 59(6): 641-646.
[8]	丁悦, 刘敏, 严海娟, 吴若函, 郭晓瑜, 辛星, 刘贤金. 噻虫胺及其混配制剂对意大利蜜蜂和玉米螟赤眼蜂的急性毒性[J]. , 2015, 58(9): 1030-1036.
[9]	Leila MAHMOODI, Oroj VALIZADEGAN, Vahid MAHDAVI. 印度藏茴香植物精油对温室棉蚜的熏蒸毒性及其成分分析（英文）[J]. , 2015, 58(2): 147-153.
[10]	祝小祥，苍涛，王彦华，吴长兴，赵学平, 王强. 三唑类杀菌剂对三种赤眼蜂成蜂的急性毒性及风险评估[J]. , 2014, 57(6): 688-695.
[11]	蔺哲广，孟飞，郑火青，周婷，胡福良. 新烟碱类杀虫剂对蜜蜂健康的影响[J]. , 2014, 57(5): 607-615.
[12]	陈澄宇，赵云贺，李慧，张鹏，慕卫，刘峰. 苯并噻唑对不同虫态韭菜迟眼蕈蚊的生物活性[J]. , 2014, 57(1): 45-51.
[13]	宋月芹, 董钧锋, 孙会忠. 亚致死浓度氯虫苯甲酰胺可降低亚洲玉米螟的种群增长[J]. , 2013, 56(4): 446-451.
[14]	金华超, 尹晓辉, 朱国念. 四种杀菌剂对玉米螟赤眼蜂酚氧化酶活性的影响[J]. , 2013, 56(2): 136-144.
[15]	黄圣卓, 公维昌, 马青云, 牛洋, 梅文莉, 陈高, 赵友兴. 云南锦斑蛾幼虫体表分泌物氰苷类成分的分离与鉴定及其对黑头酸臭蚁的生物活性[J]. , 2013, 56(2): 207-211.

樟脑罗勒生物活性提取物对储藏豇豆和大豆中四纹豆象的影响(英文)

Effects of bioactive extracts of Ocimum canum (Lamiaceae) on Callosobruchus maculatus (Coleoptera: Chrysomelidae) in stored cowpea and soybean (In English)

PDF (PC)

赞

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

Metrics

本文评价

推荐阅读 10