›› 2017, Vol. 60 ›› Issue (12): 1439-1446.doi: 10.16380/j.kcxb.2017.12.010

• 研究论文 • 上一篇    下一篇

西花蓟马成虫昼间交配习性和聚集信息素释放节律

李晓维1, 孙冉冉2, 章金明1, 张治军1, 吕要斌1,2,*   

  1. (1. 浙江省农业科学院植物保护与微生物研究所, 浙江省植物有害生物防控重点实验室——省部共建国家重点实验室培育基地, 杭州310021; 2. 南京农业大学植物保护学院, 南京 210095)
  • 出版日期:2017-12-20 发布日期:2017-12-20

Mating behavior and aggregation pheromone release rhythm of Frankliniella occidentalis (Thysanoptera: Thripidae) adults during photophase

LI Xiao-Wei1, SUN Ran-Ran2, ZHANG Jin-Ming1, ZHANG Zhi-Jun1, LU Yao-Bin1,2,*   

  1.  (1. State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China; 2. Department of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China)
  • Online:2017-12-20 Published:2017-12-20

摘要: 【目的】西花蓟马Frankliniella occidentalis是一种毁灭性的世界性入侵害虫,对我国蔬菜和花卉等作物具有重大威胁。为了更好地应用蓟马聚集信息素进行西花蓟马监测和防治,本试验在室内对西花蓟马昼间交配习性、雌雄互作和雄成虫聚集信息素释放节律进行研究。【方法】通过单独配对和群体配对两种方法研究西花蓟马成虫在光照阶段的第1, 4 , 8和12小时4个不同时间的交配行为;采用显微摄像系统研究西花蓟马雌雄成虫间4个不同时间的互作节律;采用固相微萃取法和气质联用仪分析西花蓟马雄成虫聚集信息素两种组分neryl (S)-2-methylbutanoate和(R)-lavandulyl acetate在4个不同时间的释放节律。【结果】西花蓟马成虫在光照阶段的4个不同时间内的交配率、交配历期(持续时间)、雌雄互作次数和雄成虫对雌成虫的交配尝试次数均不存在显著差异,说明交配行为和雌雄互作行为不具有明显的节律性。西花蓟马雄成虫聚集信息素具有引诱活性的neryl (S)-2-methylbutanoate在光照阶段的这4个不同时间的释放率不存在显著差异,其释放节律与西花蓟马成虫的交配相关行为相一致。另一种不具有引诱活性的组分(R)-lavandulyl acetate在光照阶段的这4个不同时间释放率具有显著差异,呈逐渐增高趋势。【结论】本研究证实了西花蓟马成虫在室内光照条件下交配相关行为不存在明显的节律性,其聚集信息素主要引诱成分neryl (S)-2-methylbutanoate的释放亦无明显的节律性。该结果为进一步研究西花蓟马聚集信息素不同组分的行为调控作用提供了理论依据,同时为合理高效地应用聚集信息素进行西花蓟马田间种群动态监测和防控提供指导。

关键词: 西花蓟马, 交配行为, 雌雄互作, 聚集信息素, 释放节律

Abstract: 【Aim】 Western flower thrips, Frankliniella occidentalis, is a worldwide invasive pest causing devastating damage on crops. Now it becomes a big threat to vegetable and ornamental industry in China. In order to establish the basis of using aggregation pheromone to monitor and control this pest, the mating activity, the female-male interaction and release rhythm of aggregation pheromoneofmaleadultsof F. occidentalis during photophase were investigated in this study. 【Methods】 The mating behavior of F. occidentalis adults was tested using single pair mating and group mating methods, the female-male interactions were observed under video system, and the release rhythms of aggregation pheromone in male adults were analyzed at four time (the 1st, 4th, 8th and 12th hour) of photophase using solid phase micro-extraction and gas chromatography-mass spectrometry. 【Results】 The mating percentages, mating duration, femalemale interaction repeats and male harassment repeats of F. occidentalis adults at the four time of photophase were not significantly different, suggesting that the corresponding behaviors are arrhythmic during photophase. The release rhythms of the attractive aggregation pheromone component neryl (S)-2-methylbutanoate were in accordance with the behavior pattern, without significant difference at the four time of photophase. However, the release rates of unattractive aggregation pheromone component (R)-lavandulyl acetate were significantly different at the four time of photophase. 【Conclusion】 The study confirmed that the mating activity, female-male interaction and the release of the attractive aggregation pheromone component neryl (S)-2-methylbutanoate of F. occidentalis adults are arrhythmic during photophase. These results provide a theoretical basis for understanding the behavioral function of aggregation pheromone compounds and a guide for population monitoring and pest control of thrips using aggregation pheromones in the field.
 

Key words: Frankliniella occidentalis; mating behavior, female-male interaction, aggregation pheromone, release rhythm