›› 2018, Vol. 61 ›› Issue (4): 432-438.doi: 10.16380/j.kcxb.2018.04.005

• 研究论文 • 上一篇    下一篇

小金蝠蛾幼虫血细胞的种类、形态及细胞免疫功能

倪若尧1,2, 孟茜2, 张寰2, 张继红2,*, 秦启联2,*   

  1. (1. 河北大学生命科学学院, 河北保定 071002; 2. 中国科学院动物研究所, 农业虫害鼠害综合治理研究国家重点实验室, 北京 100101)
  • 出版日期:2018-04-20 发布日期:2018-04-20

Types, morphology and cellular immune functions of hemocytes in larvae of Thitarodes xiaojinensis (Lepidoptera: Hepialidae)

NI Ruo-Yao1,2, MENG Qian2, ZHANG Huan2, ZHANG Ji-Hong2,*, QIN Qi-Lian2,*   

  1. (1. College of Life Sciences, Hebei University, Baoding, Hebei 071002, China; 2. State Key Laboratory of Integrated Pest Management, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China)
  • Online:2018-04-20 Published:2018-04-20

摘要: 【目的】本研究旨在鉴定小金蝠蛾Thitarodes xiaojinensis幼虫血细胞类型,统计各类血细胞所占比例并检测其细胞免疫能力。【方法】利用光学相差显微镜观察小金蝠蛾7龄幼虫血细胞,并根据鳞翅目昆虫血细胞特有形态对小金蝠蛾幼虫血细胞进行鉴定;利用96孔板对小金蝠蛾各类血细胞进行计数并计算各类血细胞所占的比例;利用酿酒酵母Saccharomyces cerevisiae以及Congo Red染色的DEAE-Sephadex A-25凝胶珠刺激小金蝠蛾7龄幼虫,检验其细胞免疫反应。【结果】小金蝠蛾幼虫血细胞由粒细胞、浆细胞、珠血细胞和类绛色细胞组成,所占比例分别为62.5%, 26.6%, 10.1%和0.8%,未发现典型的原血细胞。酿酒酵母刺激结果显示,小金蝠蛾幼虫血细胞可以发生吞噬以及结节反应。Congo Red染色的DEAE-Sephadex A-25凝胶珠刺激结果显示,小金蝠蛾幼虫血细胞可以发生包囊反应。【结论】小金蝠蛾幼虫血细胞组成及其形态与其他鳞翅目昆虫一致,其中粒细胞所占的比例最高。小金蝠蛾幼虫血细胞对酿酒酵母刺激敏感,可发生吞噬和结节反应,对Congo Red染色的DEAE-Sephadex A-25凝胶珠刺激敏感,可发生包囊反应。小金蝠蛾细胞免疫功能完善。

关键词: 小金蝠蛾, 血细胞, 细胞免疫, 吞噬, 结节, 包囊

Abstract:  【Aim】 This study aims to identify the types of hemocytes, to determine their proportions in the hemolymph of larvae of the ghost moth, Thitarodes xiaojinensis, and to examine the cellular immune functions of hemocytes. 【Methods】 The morphology of hemocytes of T. xiaojinensis larvae was observed under optical phase contrast microscopy and compared with the characteristic morphology of various hemocytes of lepidopteran insects. The cellular immune responses of hemocytes in the 7th instar larvae of T. xiaojinensis were examined after stimulating them with Saccharomyces cerevisiae and Congo Red stained DEAE-Sephadex A-25. 【Results】 The hemocytes of T. xiaojinensis larvae are composed of granular hemocytes, plasmatocytes, spherule cells and oenocytoids, accounting for 62.5%, 26.6%, 10.1% and 0.8%, respectively. The typical prohemocytes were not observed. The results of stimulation with S. cerevisiae showed that the hemocytes of T. xiaojinensis larvae could react with phagocytosis and nodulation. The results of stimulation with Congo Red stained DEAE-Sephadex A-25 showed that the hemocytes in T. xiaojinensis larvae had the ability of encapsulation. 【Conclusion】 The composition and morphology of hemocytes of T. xiaojinensis larvae are the same as those of other lepidopteran insects, and the granular hemocytes have the highest proportion. Hemocytes in T. xiaojinensis larvae are sensitive to the stimulation of S. cerevisiae and Congo Red stained DEAE-Sephadex A-25. The former causes phagocytosis and nodulation, while the latter causes encapsulation. The function of cellular immunity in T. xiaojinensis is typical.

Key words: Thitarodes xiaojinensis, hemocytes, cellular immunity, phagocytosis, nodulation, encapsulation