姜黄根提取物对苜蓿蚜成蚜有杀虫活性化合物的分离与鉴定

doi:10.16380/j.kcxb.2018.06.008

›› 2018, Vol. 61 ›› Issue (6): 698-703.doi: 10.16380/j.kcxb.2018.06.008

姜黄根提取物对苜蓿蚜成蚜有杀虫活性化合物的分离与鉴定

张敏, 刘佳, 傅伟杰, 代光辉^*

（上海交通大学农业与生物学院, 植物健康与天然产物实验室, 农村农业部都市农业重点实验室, 上海 200240）

出版日期:2018-06-20 发布日期:2018-06-20

Isolation and identification of insecticidal compounds from Curcuma longa roots against Aphis craccivora (Hemiptera: Aphididae) adults

ZHANG Min, LIU Jia, FU Wei-Jie, DAI Guang-Hui^*

(Plant Health and Natural Products Laboratory, Key Laboratory of Urban Agriculture, Ministry of Agriculture and Rural Affairs, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China)

Online:2018-06-20 Published:2018-06-20

摘要/Abstract

摘要： 【目的】明确姜黄Curcuma longa根乙醇提取物对苜蓿蚜Aphis craccivora成蚜的生物活性和活性成分，并鉴定活性成分的结构，为利用姜黄根提取物研制新型植物源杀虫剂防治苜蓿蚜奠定基础。【方法】运用植物化学手段和生物活性跟踪方法，采用萃取、硅胶柱层析、葡聚糖凝胶层析，并结合薄层层析检测方法从姜黄根提取物中分离、纯化出对苜蓿蚜成蚜具有杀虫活性的化合物，并采用核磁共振和质谱对其进行结构鉴定。通过浸渍法测定姜黄根提取物对苜蓿蚜无翅成蚜的触杀活性，通过点滴法测定活性成分对苜蓿蚜无翅成蚜的触杀活性。【结果】姜黄根95%乙醇提取物对苜蓿蚜无翅成蚜具有触杀活性，处理后24 h的LC₅₀值为3 226.27 mg/L。从姜黄根中分离得到的活性化合物为芳姜黄酮，该物质对苜蓿蚜无翅成蚜表现出较好的触杀活性，处理后24 h的LC₅₀值为706.10 mg/L。【结论】姜黄根95%乙醇提取物对苜蓿蚜成蚜具有一定的杀虫活性，芳姜黄酮是主要的活性物质。

关键词: 姜黄, 根, 苜蓿蚜, 芳姜黄酮, 杀虫化合物, 杀虫活性

Abstract: 【Aim】 This study aims to determine the insecticidal activity of the ethanol extract from Curcuma longa roots against Aphis craccivora adults, to identify the structure of the active compounds, and to evaluate the control effect of the active compound against this insect so as to establish basic data for its application in a botanical pesticide. 【Methods】 Insecticidal compounds were isolated and purified from the extract of C. longa roots with activity-guided fractionation by extraction, silica gel column chromatography, thin layer chromatography and Sephadex LH-20 column chromatography, and their structures were identified based on NMR (nuclear magnetic resonance) and MS (mass spectrometry) data. The insecticidal activities of the extract and the active compounds against apterous adults of A. craccivora were evaluated by dipping method and topical application, respectively. 【Results】 The 95% ethanol extract of C. longa roots had an contact activity against the apterous adults of A. craccivora, with the LC₅₀value of 3 226.27 mg/L at 24 h after treatment. The active compound (+)-(S)-ar-turmerone isolated from the extract of C. longa roots exhibited good contact activity against the apterous adults of A. craccivora, with the LC₅₀ value of 706.10 mg/L at 24 h after treatment. 【Conclusion】 The 95% ethanol extract of C. longa roots has certain insecticidal activity against A. craccivora adults, and (+)-(S)-ar-turmerone is the main active compound.

Key words: Curcuma longa, root, Aphis craccivora, (+)-(S)-ar-turmerone, insecticidal compounds, insecticidal activity

张敏, 刘佳, 傅伟杰, 代光辉. 姜黄根提取物对苜蓿蚜成蚜有杀虫活性化合物的分离与鉴定[J]. , 2018, 61(6): 698-703.

ZHANG Min, LIU Jia, FU Wei-Jie, DAI Guang-Hui. Isolation and identification of insecticidal compounds from Curcuma longa roots against Aphis craccivora (Hemiptera: Aphididae) adults[J]. , 2018, 61(6): 698-703.

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链接本文: http://www.insect.org.cn/CN/10.16380/j.kcxb.2018.06.008

http://www.insect.org.cn/CN/Y2018/V61/I6/698

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姜黄根提取物对苜蓿蚜成蚜有杀虫活性化合物的分离与鉴定

Isolation and identification of insecticidal compounds from Curcuma longa roots against Aphis craccivora (Hemiptera: Aphididae) adults

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