›› 2018, Vol. 61 ›› Issue (10): 1160-1169.doi: 10.16380/j.kcxb.2018.10.005

• 研究论文 • 上一篇    下一篇


唐芬芬1, 2, 张永红1, 邵榆岚1, 朱峰1, 白兴荣1,*   

  1.  (1. 云南省农业科学院蚕桑蜜蜂研究所, 云南蒙自 661101; 2. 云南农业大学植物保护学院, 云南生物资源保护与利用国家重点实验室, 昆明 650201)
  • 出版日期:2018-10-20 发布日期:2018-10-20

Virulence and phylogenetic analysis of Bombyx mori nucleopolyhedrovirus isolates from Yunnan, southwestern China

TANG Fen-Fen1, 2, ZHANG Yong-Hong1, SHAO Yu-Lan1, ZHU Feng1, BAI Xing-Rong1,*    

  1. (1. Institute of Sericulture and Apiculture, Yunnan Academy of Agricultural Sciences, Mengzi, Yunnan 661101, China; 2. State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, College of Plant Protection, Yunnan Agricultural University, Kunming 650201, China)
  • Online:2018-10-20 Published:2018-10-20

摘要: 【目的】由家蚕核型多角体病毒(Bombyx mori nucleopolyhedrovirus, BmNPV)侵染家蚕Bombyx mori导致的核型多角体病(血液型脓病)在云南蚕区普遍发生,危害严重,给云南蚕业带来严重的经济损失。本研究旨在测定云南不同蚕区BmNPV分离株的毒力及了解BmNPV在云南的病害流行趋势,为监测和控制云南家蚕血液型脓病打下基础。【方法】通过BmNPV对家蚕5龄幼虫致死率和对家蚕BmN细胞毒力的测定评价了云南蚕区19个BmNPV分离株的致病力;对19个云南BmNPV分离株的bro-d基因进行克隆、测序及系统进化分析。【结果】BmNPV对家蚕5龄幼虫的致死率测定表明,云南不同地区BmNPV毒株对家蚕的口服感染力差异较大;细胞毒力测定结果显示不同BmNPV分离株的出芽型病毒粒子(budded virus, BV)滴度差异较大。进化分析表明,所获取来自云南的19个BmNPV分离株主要分为两个亚群,亚群I在云南省的东部、中部和西部地区均有分布,而亚群II主要集中分布于云南省北部地区。从地理分布图可以看出,亚群I所在地区多为亚热带,温度偏高;而亚群II所处的云南北部地区多为典型高原季风气候,温度相对偏低。【结论】云南蚕区存在丰富的BmNPV株系,各分离株对家蚕的毒力差异较大;云南BmNPV分离株的进化关系在一定程度上与地理气候密切相关。

关键词: 家蚕, 蚕区, BmNPV, 生物测定; bro-d, 云南

Abstract: 【Aim】 Bombyx mori nucleopoyhedrovirus (BmNPV) causes nuclear polyhedrosis which is one of the most important prevalent diseases and results in significant economic losses in sericultural areas in Yunnan, southwestern China. This study aims to determinate the virulence of BmNPV isolates from Yunnan and to investigate the epidemic trend of BmNPV diseases in Yunnan, so as to provide a basis for surveillance and control of the B. mori nuclear polyhedrosis. 【Methods】 The infectivity of 19 BmNPV isolates from Yunnan was estimated by the mortality of the 5th instar larvae of B. mori and the virulence to BmN cells caused by BmNPV. The bro-d genes of 19 BmNPV isolates were cloned, sequenced and subjected to phylogenetics analysis. 【Results】 The per os infectivity varied in different BmNPV isolates from Yunnan by comparing the mortality of the 5th instar larvae of B. mori caused by BmNPV isolates. The virulence test results showed that the titers of budded virus (BV) of the 19 BmNPV isolates were significantly different. Phylogenetic analysis showed that the 19 BmNPV isolates from Yunnan were separated into two main clades, i.e., clade I and II. Clade I isolates were distributed in eastern, central and western parts of Yunnan province, which are sub-tropical regions with high temperature, while clade II isolates were mainly distributed in northern part of Yunnan province, which has plateau moosoon climate with relatively low temperature. 【Conclusion】 There are abundant BmNPV strains in sericultural areas of Yunnan. The virulence of BmNPV isolates from Yunnan to B. mori varies widely, and the evolutionary relationship of these isolates is related with geography and climate in a certain degree.

Key words: Bombyx mori, sericultural area, BmNPV, bioassay, bro-d; Yunnan