玉米桃蛀螟雌蛾性信息素的微量组分鉴定及其性信息素诱芯组分的配比优化

doi:10.16380/j.kcxb.2018.10.008

›› 2018, Vol. 61 ›› Issue (10): 1184-1191.doi: 10.16380/j.kcxb.2018.10.008

玉米桃蛀螟雌蛾性信息素的微量组分鉴定及其性信息素诱芯组分的配比优化

徐翔¹, 孔德生², 冯波³, 钱凯³, 杜永均^4,*

(1. 四川省农业厅植物保护站 , 成都 610041; 2. 山东省邹城市植物保护站, 山东邹城 273500; 3. 温州医科大学健康与环境生态研究所, 浙江温州 325035; 4. 浙江大学农药与环境毒理研究所, 杭州 310058)

出版日期:2018-10-20 发布日期:2018-10-20

Identification of the trace sex pheromone components of Conogethes punctiferalis (Lepidoptera: Crambidae) female adults on maize and optimization of their ratio in sex pheromone lures used in maize fields

XU Xiang¹, KONG De-Sheng², FENG Bo³, QIAN Kai3, DU Yong-Jun^4,*

(1. Sichuan Provincial Plant Protection Station, Department of Agriculture, Chengdu 610041, China; 2. Zoucheng Plant Protection Station, Shangdong Province, Zoucheng, Shandong 273500, China;3. Institute of Health and Environmental Ecology, Wenzhou Medical University, Wenzhou, Zhejiang 325035, China; 4. Institute of Pesticide and Environmental Toxicology, Zhejiang University, Hangzhou 310058, China)

Online:2018-10-20 Published:2018-10-20

摘要/Abstract

摘要： 【目的】明确为害玉米的桃蛀螟Conogethes punctiferalis雌蛾性信息素完整组成以及嗅觉反应特点。【方法】采集为害玉米的桃蛀螟性成熟雌蛾，利用气质联用仪(GCMS)对其性腺提取物和合成标样进行比较分析，触角电位(EAG)记录技术测定和分析嗅觉反应，并结合2014年在四川和山东进行的田间诱捕比较试验筛选出最佳性信息素混合物。【结果】E10-16∶Ald为玉米桃蛀螟雌蛾性信息素主要组分，同时还检测到微量的Z10-16∶Ald和Z10-16∶OH。未交配雌蛾对E10-16∶Ald, 16∶Ald, Z10-16∶ Ald, Z10-16∶OH和E10-16∶OH这5种性信息素化合物的嗅觉反应不明显，而未交配雄蛾对 E10-16∶Ald和Z10-16∶Ald的嗅觉反应强烈，对E10-16∶OH和Z10-16∶OH的嗅觉反应次之。2014年在四川和山东用不同比例的三组分(E10-16∶Ald, Z10-16∶Ald和16∶Ald)和四组分(E10-16∶Ald, Z10-16∶Ald, 16∶Ald和E10-16∶OH)诱芯进行的田间试验中，三组分诱芯中E10-16∶Ald, Z10-16 ∶Ald和16∶Ald的比例在95∶5∶10时诱虫量最多；四组分诱芯中微量组分Z10-16∶OH在四川未表现出明显的增效作用，而在山东表现出明显的增效作用，以E10-16∶Ald, Z10-16∶Ald, 16∶Ald和 E10-16∶OH的比例为95∶5∶10∶5时诱蛾能力最强。【结论】玉米桃蛀螟的性信息素完整组成包括E10 -16∶Ald, Z10-16∶Ald, 16∶Ald和E10-16∶OH，其最佳比例为95∶5∶10∶5。

关键词: 桃蛀螟, 寄主植物, EAG, 性信息素, 地理区系差异, 诱芯

Abstract: 【Aim】 The purpose of this study is to further clarify the complete composition of female sex pheromone of the yellow peach moth, Conogethes punctiferalis, infesting maize and its olfactory profile. 【Methods】 The extracts of pheromone glands from sexually mature female moths of C. punctiferalis infesting maize were analyzed by gas chromatography -mass spectrometry (GC-MS), and sex pheromone components were confirmed with the synthetic compounds. The olfactory responses were detected and analyzed by electroantennogram recording technique. The mixtures of sex pheromone components were screened and optimized by field trapping tests in Sichuan and Shandong in 2014. 【Results】 E10-16∶Ald was the main component of sex pheromone of female adults of C. punctiferalis on maize, while traces of Z10-16∶Ald and Z10-16∶OH were also detected. The unmated female adults did not respond to the five sex pheromone compounds including E10-16∶Ald, 16∶Ald, Z10-16∶Ald, Z10-16∶OH and E10-16∶OH, while the unmated male adults responded strongly to E10-16∶Ald and Z10-16∶Ald, and also responded to E10-16∶OH and Z10-16∶OH. In the field tests with lures containing three components (E10-16∶Ald, Z10-16∶Ald and 16∶Ald) and four components (E10-16∶Ald, Z10-16∶Ald, 16∶Ald and E10-16∶ OH) at different ratios in Sichuan and Shandong in 2014, the lure containing E10-16∶Ald, Z10-16∶Ald and 16∶Ald at the ratio of 95∶5∶10 trapped the highest number of adults. The trace component Z10-16∶OH in Sichuan showed no significantly synergistic effect, while showed significantly synergistic effect in Shandong, and the lure containing E10-16 ∶Ald, Z10-16∶Ald, 16∶Ald and E10-16∶OH at the ratio of 95∶5∶10∶5 showed the highest trapping efficacy. 【Conclusion】 The complete composition of the sex pheromone of C. punctiferalis on maize includes E10-16∶Ald, Z10-16∶Ald, 16∶Ald and E10-16∶OH, and their optimal ratio is 95∶5∶10∶5.

Key words: Conogethes punctiferalis, host plant, EAG, sex pheromone, geographical variation, lure

徐翔, 孔德生, 冯波, 钱凯, 杜永均. 玉米桃蛀螟雌蛾性信息素的微量组分鉴定及其性信息素诱芯组分的配比优化[J]. , 2018, 61(10): 1184-1191.

XU Xiang, KONG De-Sheng, FENG Bo, QIAN Kai, DU Yong-Jun. Identification of the trace sex pheromone components of Conogethes punctiferalis (Lepidoptera: Crambidae) female adults on maize and optimization of their ratio in sex pheromone lures used in maize fields[J]. , 2018, 61(10): 1184-1191.

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http://www.insect.org.cn/CN/Y2018/V61/I10/1184

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玉米桃蛀螟雌蛾性信息素的微量组分鉴定及其性信息素诱芯组分的配比优化

Identification of the trace sex pheromone components of Conogethes punctiferalis (Lepidoptera: Crambidae) female adults on maize and optimization of their ratio in sex pheromone lures used in maize fields

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