昆虫学报 ›› 2019, Vol. 62 ›› Issue (1): 73-81.doi: 10.16380/j.kcxb.2019.01.008

• 研究论文 • 上一篇    下一篇

黑腹果蝇对高盐的产卵避性反应及其生物学意义

高露1,#, 李钊2,#, 赫晓媛4, 甘颖3, 金虹云2, 闫琴2, 白芃2,*, 刘威3,*    

  1. (1. 山西医科大学汾阳学院眼视光学系, 山西汾阳 032200; 2. 山西医科大学汾阳学院基础医学部, 山西汾阳 032200; 3. 山西医科大学汾阳学院医学检验系, 山西汾阳 032200; 4. 山西医科大学汾阳学院护理学系, 山西汾阳 032200)
  • 出版日期:2019-01-20 发布日期:2019-01-24

Oviposition avoidance of Drosophila melanogaster to high salt and its biological significance

GAO Lu1,#, LI Zhao2,#, HE Xiao-Yuan4, GAN Ying3, JIN Hong-Yun2, YAN Qin2, BAI Peng2,*, LIU Wei3,*    

  1.  (1. Department of Ophthalmology & Optometry Medica, Fenyang College, Shanxi University, Fenyang, Shanxi 032200, China; 2. Department of Faculty of Basic Medicine, Fenyang College, Shanxi University, Fenyang, Shanxi 032200, China; 3. Department of Medical Laboratory Science, Fenyang College, Shanxi Medical University, Fenyang, Shanxi 032200, China; 4. Department of Nursing, Fenyang College, Shanxi Medical University, Fenyang, Shanxi 032200, China)
  • Online:2019-01-20 Published:2019-01-24

摘要:

【目的】研究黑腹果蝇Drosophila melanogaster对高盐的产卵避性,并通过高盐下后代生存率和发育历期的变化来揭示这一现象的生物学意义。【方法】应用产卵双选择装置,检测黑腹果蝇和雅库巴果蝇D. yakuba雌成虫分别对含有0.25和1 mol/L NaCl的培养基的产卵选择性;利用产卵装置,检测黑腹果蝇对1 mol/L NaCl的位置效应;通过强迫产卵实验检测钠盐胁迫下黑腹果蝇的产卵能力;通过毛细管摄食法和观察口器延伸,检测钠盐胁迫下黑腹果蝇的摄食行为。利用黑暗条件和突变体Orco2分别探究视觉和嗅觉对高盐的产卵选择性。通过外科手术法摘除黑腹果蝇前足部分味觉感受器,并使用咸味突变体Ionotropic Receptor 76b (IR76b),研究介导该行为的感觉系统。在含有不同浓度NaCl (0, 0.25, 0.50, 0.75和1 mol/L)的培养基上培养黑腹果蝇,探究NaCl对黑腹果蝇后代的发育历期和存活率的影响。【结果】雌性黑腹果蝇产卵对高盐产生避性反应,对0.25和1 mol/L NaCl的产卵偏嗜指数分别为-0.45和-0.59;雅库巴果蝇对高盐的反应与黑腹果蝇相同,对0.25和1 mol/L NaCl的产卵偏嗜指数分别为-0.78和-0.99;并且两种果蝇均对0.25和1 mol/LKCl和CaCl2产生产卵避性反应。黑腹果蝇避开含0.25和1 mol/L NaCl培养基,位置指数分别为-0.74和-0.88。高盐不影响黑腹果蝇的生育能力,其对0, 0.25和1 mol/L NaCl的产卵指数分别为5.59, 6.63和5.41。黑腹果蝇对0.25和1 mol/L NaCl摄食产生明显排斥反应,摄食指数分别为-0.37和-0.41,且随浓度增大食欲越低。同时在黑暗条件下,黑腹果蝇对0.25和1 mol/L NaCl仍产生了显著的避性反应,产卵偏嗜指数分别为-0.49和-0.59,摘除前足的黑腹果蝇对0.25 mol/L NaCl的产卵偏嗜指数为-0.05,IR76b突变体对高盐的产卵避性反应消失,对0.25和1 mol/L NaCl的产卵偏嗜指数分别为0.46和0.39。0.25 mol/L NaCl明显延长了后代成蛹时间0.40 d,后代幼虫存活率降低了16.5%,而在1 mol/L NaCl上后代卵完全不能形成蛹和成虫。【结论】黑腹果蝇雌成虫通过咸味感觉器官感知高盐而产生产卵避性反应,以促进后代的生长发育和提高后代的幼虫存活率。

关键词: 黑腹果蝇, 高盐, 产卵避性, 味觉系统, 发育历期, 存活率

Abstract: 【Aim】 To study the oviposition avoidance of Drosophila melanogaster to high salt, and to explore the biological significance of this phenomenon by the changes of the survival rate and developmental duration of the offspring under high salt. 【Methods】 The oviposition preference of female adults of D. melanogaster and D. yakuba to culture media containing 0.25 and 1 mol/L NaCl was assayed by the twochoice apparatus. The location effect of 1 mol/L NaCl on D. melanogaster adults was detected with oviposition apparatus. The oviposition ability of D. melanogaster adults under salt stress was detected with forced oviposition assay. The feeding behavior of D. melanogaster under salt stress was examined with capillary feeding and mouthpart extension assays. The visional and olfactory perceptions of oviposition selection were carried out in darkness and with Orco2 mutant, respectively. The sensory system mediating this behavior was studied by surgically removing the partial taste receptor of D. melanogaster forelegs and using the saline taste mutant Ionotropic Receptor 76b (IR76b). D. melanogaster was cultured on the media containing a serial of concentrations of NaCl (0, 0.25, 0.50, 0.75 and 1 mol/L), and the effects of NaCl on the developmental duration and survival rate of the offspring of D. melanogaster were investigated. 【Results】 The female adults of D. melanogaster avoided to lay eggs on the media containing 0.25 and 1 mol/L NaCl, with the oviposition indexes of -0.45 and -0.59, respectively. D. yakuba adults created similar response to high salt, with the oviposition preference indexes of -0.78 and -0.99, respectively. Both species tested showed oviposition avoidance to high concentration of KCl and CaCl2. D. melanogaster avoided to stay at the media containing 0.25 and 1 mol/L NaCl, with the location indexes of -0.74 and -0.88, respectively. There were comparable numbers of eggs laid on the media containing 0, 0.25 and 1 mol/L NaCl. D. melanogaster adults rejected 0.25 and 1 mol/L NaCl, with the feeding indexes of -0.37 and -0.41, respectively, and their appetite decreased as the concentration increased. Under darkness, D. melanogaster adults still showed a significant avoidance response to 0.25 and 1 mol/L NaCl, with the oviposition indexes of -0.49 and -0.59, respectively. After the forelegs of D. melanogaster adults were removed, the oviposition avoidance response of flies to 0.25 mol/L NaCl was impaired, with the oviposition index of -0.05. The oviposition avoidance response of IR76b mutant to 0.25 and 1 mol/L NaCl was compromised, with the oviposition indexes of 0.46 and 0.39, respectively. The time to puparium formation of the offspring on the media containing 0.25 mol/L NaCl was significantly prolonged by 0.40 d as compared to the control, and the larval survival rate of the offspring was decreased by 16.5%. The eggs of the offspring completely failed to form pupa and adults on the medium containing 1 mol/L NaCl. 【Conclusion】 Female adults of D. melanogaster display the oviposition avoidance to high salt through salt taste perception, thus promoting the development and larval survival of the offspring.

Key words: Drosophila melanogaster, high salt, oviposition avoidance, gustatory system, developmental duration, survival rate