• 研究论文 •

### 重阳木锦斑蛾越冬幼虫的耐寒性变化

1. (河南科技大学林学院, 河南洛阳 471000)
• 出版日期:2019-08-20 发布日期:2019-08-29

### Changes in the cold hardiness of overwintering larvae of Histia rhodope (Lepidoptera: Zygaenidae)

YANG Hai-Bo, HU Zhen-Jie, DONG Jun-Feng, ZHU Pin-Hong, LI Ding-Xu*

1. (Forestry College, Henan University of Science and Technology, Luoyang, Henan 471000, China)
• Online:2019-08-20 Published:2019-08-29

【目的】重阳木锦斑蛾Histia rhodope是为害重阳木Bischofia polycarpa的重要害虫之一。本研究旨在了解重阳木锦斑蛾幼虫的抗寒能力，并为探讨抗寒机理提供理论基础。【方法】在越冬期的不同阶段(2017年11月7日、2017年12月7日、2018年1月5日、2018年2月4日和2018年3月5日)分别采集室外重阳木锦斑蛾越冬幼虫，对其体重、过冷却点、结冰点、含水量、脂肪、总糖和总蛋白质含量进行测定。【结果】重阳木锦斑蛾幼虫的过冷却点在越冬期不同月份有显著差异，与环境温度呈显著正相关(P<0.05)，最低值出现在1月份，为-15.26℃，最高值出现在3月份，为-13.30℃；结冰点变化趋势与过冷却点一致；体重和脂肪含量在越冬期间逐渐下降，与过冷却点无相关性(P>0.05)；过冷却能力随着虫体游离水含量的升高而降低，随其降低而升高，而结合水含量的变化趋势恰好相反；总糖含量在11月最高，为14.95 μg/mg，显著高于3月份的5.07 μg/mg；总蛋白质含量在越冬期间呈先升高后降低的趋势，在1月份含量最高，为23.66 μg/mg，显著高于11月份的含量(16.69 μg/mg)。【结论】重阳木锦斑蛾幼虫的耐寒性在越冬期间随气温的降低逐渐增强，随气温的回升又逐渐减弱；蛋白质可能是该虫重要的耐寒物质。

Abstract: 【Aim】 Histia rhodope is one of the major pests of Bischofia polycarpa. This study aims to investigate the cold hardiness of H. rhodope larvae and to provide a theoretical basis for exploring its cold resistance mechanisms. 【Methods】 The body weight, supercooling point (SCP), freezing point (FP) and contents of water, fat, total sugar and total protein in overwintering larvae of H. rhodope were measured at different overwintering stages (November 7, 2017, December 7, 2017, January 5, 2018, February 4, 2018, and March 5, 2018). 【Results】 There were significant differences in the SCP of H. rhodope larvae among the different overwintering months, and SCP showed a significant positive correlation with the ambient temperature (P<0.05). The lowest mean SCP (-15.26℃) occurred in January, and the highest (-13.30℃) occurred in March. The variation tendency of FP was consistent with that of SCP. The fresh weight and fat content decreased gradually during the overwintering period, and there was no correlation between the SCP and the fresh weight and fat content (P>0.05). The supercooling capacity increased as the free water content decreased, while reduced as the free water content increased. However, the change trend of bound water content was opposite to that of the free water content. The total sugar content in November was the highest (14.95 μg/mg), which was significantly higher than that in March (5.07 μg/mg). The total protein content increased first and then decreased during overwintering, and reached the highest value in January (23.666 μg/mg), which was significantly higher than that in November (16.69 μg/mg). 【Conclusion】 The cold hardiness of overwintering larvae of H. rhodope increases gradually with the decrease of ambient temperature and then weakens with the increase of ambient temperature. Proteins may be the important cold-resistant substance in H. rhodope.