水稻条纹花叶病毒对介体电光叶蝉生长繁殖及取食行为的影响

doi:10.16380/j.kcxb.2020.02.007

昆虫学报 ›› 2020, Vol. 63 ›› Issue (2): 174-180.doi: 10.16380/j.kcxb.2020.02.007

水稻条纹花叶病毒对介体电光叶蝉生长繁殖及取食行为的影响

李盼¹, 张洁², 岳玥¹, 陈红燕¹, 吴维¹, 魏太云¹, 贾东升^1,*

(1. 福建农林大学植物保护学院, 福建省植物病毒学重点实验室, 福州350002; 2. 云南省农业科学院生物技术与种质资源研究所, 昆明 650000)

出版日期:2020-02-20 发布日期:2020-02-25

Effects of Rice stripe mosaic virus on the growth, reproduction and feeding behavior of the vector Recilia dorsalis (Hemiptera: Cicadellidae)

LI Pan¹, ZHANG Jie², YUE Yue¹, CHEN Hong-Yan¹, WU Wei¹, WEI Tai-Yun¹, JIA Dong-Sheng^1,*

(1. Fujian Province Key Laboratory of Plant Virology, College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou 350002, China; 2. Biotechnology and Germplasm Resources Institute, Yunnan Academy of Agricultural Sciences, Kunming 650000, China)

Online:2020-02-20 Published:2020-02-25

摘要/Abstract

摘要： 【目的】由电光叶蝉Recilia dorsalis传播的水稻条纹花叶病毒(rice stripe mosaic virus, RSMV)目前在我国华南稻区大面积发生并对水稻生产造成严重危害。本研究旨在明确RSMV对介体电光叶蝉生长繁殖及取食行为的影响。【方法】通过生物学实验测定RSMV侵染后电光叶蝉的生长和生命表参数；利用刺吸电位(electrical penetration graph, EPG)技术比较携带和未携带RSMV的电光叶蝉成虫在健康水稻上的取食行为差异；采用Y型嗅觉仪测定电光叶蝉成虫对感染和未感染RSMV水稻的寄主选择倾向性。【结果】与无毒电光叶蝉相比，携带RSMV的电光叶蝉若虫发育历期延长，而若虫存活率、成虫羽化率、雌虫繁殖力和卵孵化率下降。无毒电光叶蝉成虫倾向于选择取食RSMV侵染的水稻，而带毒电光叶蝉成虫倾向于选择取食健康水稻。与无毒电光叶蝉相比，带毒电光叶蝉成虫取食健康水稻所产生的刺探波、障碍波和唾液分泌波次数和持续时间均显著增加，被动取食波和休息波次数减少但时间均延长。【结论】与无毒电光叶蝉相比，感染RSMV使带毒电光叶蝉若虫发育历期延长且不利于其种群的繁殖。RSMV通过调控介体电光叶蝉成虫的取食和寄主选择行为而有利于自身在寄主水稻间的传播。

关键词: 电光叶蝉, 水稻条纹花叶病毒, 刺吸电位, 繁殖, 取食行为；寄主选择行为

Abstract: 【Aim】 Rice stripe mosaic virus (RSMV) transmitted by the leafhopper Recilia dorsalis has caused severe rice damage in southern China. This study aims to evaluate the effects of RSMV on the growth, reproduction and feeding behavior of the vector R. dorsalis. 【Methods】 The growth and life table parameters of R. dorsalis infected by RSMV were assayed by laboratory breeding experiments, and the feeding behavior of the nonviruliferous and viruliferous R. dorsalis on healthy rice plants was measured by the electrical penetration graph (EPG) technique. Finally, the host plant preference of R. dorsalis to RSMV-free and RSMV-infected rice plants was determined with Y-shaped olfactometer. 【Results】 When the R. dorsalis nymphs were infected with RSMV, the nymphal duration of viruliferous R. dorsalis was prolonged, while the nymphal survival rate, adult emergence rate, female fecundity and egg hatching rate were reduced as compared with those of the nonviruliferous R. dorsalis. In addition, the nonviruliferous R. dorsalis adults preferred RSMV-infected rice plants to RSMV-free rice plants, while the viruliferous R. dorsalis adults preferred to select RSMV-free rice plants rather than select RSMV-infected rice plants. The viruliferous R. dorsalis adults fed on RSMV-free rice plants had a higher number and longer duration of waveforms representing stylet puncture, penetration difficulties and salivation, and also exhibited a lower number and lengthened duration of waveforms representing phloem sap ingestion phase and resting behavior as compared to the nonviruliferous leafhoppers. 【Conclusion】 The infection of RSMV lengthens the nymphal duration of viruliferous R. dorsalis compared with nonviruliferous R. dorsalis and is unfavorable to the propagation of viruliferous population. RSMV manipulates the feeding and host selection behavior of R. dorsalis adults to facilitate viral transmission between rice plants.

Key words: Recilia dorsalis; Rice stripe mosaic virus, electrical penetration graph, reproduction, feeding behavior, host selection behavior

李盼, 张洁, 岳玥, 陈红燕, 吴维, 魏太云, 贾东升. 水稻条纹花叶病毒对介体电光叶蝉生长繁殖及取食行为的影响[J]. 昆虫学报, 2020, 63(2): 174-180.

LI Pan, ZHANG Jie, YUE Yue, CHEN Hong-Yan, WU Wei, WEI Tai-Yun, JIA Dong-Sheng . Effects of Rice stripe mosaic virus on the growth, reproduction and feeding behavior of the vector Recilia dorsalis (Hemiptera: Cicadellidae)[J]. Acta Entomologica Sinica, 2020, 63(2): 174-180.

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水稻条纹花叶病毒对介体电光叶蝉生长繁殖及取食行为的影响

Effects of Rice stripe mosaic virus on the growth, reproduction and feeding behavior of the vector Recilia dorsalis (Hemiptera: Cicadellidae)

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