昆虫学报 ›› 2020, Vol. 63 ›› Issue (2): 191-198.doi: 10.16380/j.kcxb.2020.02.009

• 研究论文 • 上一篇    下一篇

nC22矿物油及其与吡虫啉混用对柑橘木虱的室内毒力评价

高晶#, 杨琼玉#, 冯耀恒, 毛润乾*   

  1. (广东省生物资源应用研究所, 广东省动物保护与资源利用重点实验室, 广东省野生动物保护和利用公共实验室, 广东省矿物油农药工程技术研究中心, 广州 510260)
  • 出版日期:2020-02-20 发布日期:2020-02-25

Evaluation of laboratory toxicities of nC22 agricultural mineral oil and its mixture with imidacloprid against the Asian citrus psyllid, Diaphorina citri (Hemiptera: Liviidae)

GAO Jing#, YANG Qiong-Yu#, FENG Yao-Heng, MAO Run-Qian*    

  1. (Guangdong Key Laboratory of Animal Conservation and Resource Utilization, Guangdong Public Laboratory of Wild Animal Conservation and Utilization, Guangdong Engineering Research Center for Mineral Oil Pesticides, Guangdong Institute of Applied Biological Resources, Guangzhou 510260, China)
  • Online:2020-02-20 Published:2020-02-25

摘要: 【目的】室内评价nC22矿物油单独使用,以及与吡虫啉混用对柑橘木虱Diaphorina citri的毒力,并筛选nC22矿物油对吡虫啉防治柑橘木虱具有增效作用的混配比例,为矿物油防治柑橘木虱的应用提供科学依据。【方法】使用nC22矿物油以及阳性对照nC23和nC28矿物油,采用浸渍法和喷雾法分别检测矿物油对柑橘木虱卵和低龄若虫、高龄若虫、成虫的致死作用,以处理后第7天(卵)和第1天(若虫和成虫)的LC50值评估毒力。将nC22矿物油与吡虫啉以不同配比混配,测定混配液对低龄若虫的毒力,使用交互测定法、共毒因子(co-toxicity factor, CTF)法和共毒系数(co-toxicity coefficient, CTC)法评价矿物油对吡虫啉的增效作用。【结果】单独使用时,nC22矿物油对卵的LC50值低于nC23和nC28矿物油;对低龄若虫和高龄若虫的LC50值与nC23矿物油相当,都低于nC28矿物油;对成虫的LC50值与nC28矿物油相当,都低于nC23矿物油。与吡虫啉混用时,nC22矿物油与吡虫啉混配比例为3∶7, 4∶6和7∶3时,矿物油对吡虫啉有增效作用:交互测定法中柑橘木虱低龄若虫的实际死亡率位于等效线的上方,CTF值分别为34.807, 22.655和40.798,CTC值分别为187.410, 183.876和222.936。3种混配液中7∶3的混配比例对吡虫啉的增效作用最强。【结论】nC22矿物油对柑橘木虱的毒力高于进口nC23矿物油及传统nC28矿物油,且以适当混配比例混用时nC22矿物油对吡虫啉具有显著增效作用,理论上可大幅减少化学杀虫剂的使用量,可进一步通过田间试验进行验证。

关键词: 柑橘木虱, 吡虫啉, 矿物油, 增效作用, 混配比例, 毒力

Abstract: 【Aim】 The objective of this study is to evaluate the toxicities of nC22 mineral oil against the Asian citrus psyllid, Diaphorina citri, when used alone or mixed with imidacloprid in the laboratory, and to screen the mixing ratios that show synergistic effect on imidacloprid, so as to provide scientific support for the application of mineral oil against D. citri. 【Methods】 In the laboratory, the lethal effect of nC22 mineral oil and the positive controls nC23 and nC28 mineral oils against eggs, and the early instar nymphs, late instar nymphs, and adults of D. citri were determined by immersion method and spraying method, respectively, and the toxicities were calculated based on the LC50 values (7 d after treatment for eggs, and 1 d after treatment for nymphs and adults). The nC22 mineral oil was mixed with imidacloprid at various ratios, and their lethal effects on the early instar nymphs of D. citri were determined. The synergistic effect of nC22 mineral oil on imidacloprid was evaluated by interactive determination, co-toxicity factor (CTF), and co-toxicity coefficient (CTC) methods. 【Results】 When used alone, the LC50 value of nC22 mineral oil against D. citri eggs was significantly lower than those of nC23 and nC28 mineral oils. The LC50values of nC22 mineral oil against both the early and late instar nymphs were similar to those of nC23 mineral oil, and lower than those of nC28 mineral oil. The LC50 value of nC22 mineral oil against adults was similar to that of nC28 mineral oil, and lower than that of nC23 mineral oil. The mixtures of nC22 mineral oil and imidacloprid at the ratios of 3∶7, 4∶6 and 7∶3 showed remarkable synergistic effect on imidacloprid: the actual mortalities of the early instar nymphs of D. citri were above the equivalent line in the interactive determination method, the CTF value were 34.807, 22.655, and 40.798, and the CTC values were 187.430, 183.876 and 222.936, respectively. The mixture of nC22 mineral oil and imidacloprid at the ratio of 7∶3 showed the strongest synergistic effect on imidacloprid. 【Conclusion】 nC22 mineral oil shows higher toxicity against D. citri than the imported nC23 and traditional nC28 mineral oils. When mixed with imidacloprid at proper ratios, nC22 mineral oil shows strong synergistic effect, and theoretically it can significantly reduce the use of chemical insecticide, which could be further tested in fields.

Key words: Diaphorina citri; imidacloprid, mineral oil, synergistic effect, mixing ratio, toxicity