七星瓢虫触角转录组及嗅觉相关基因分析

doi:10.16380/j.kcxb.2020.06.007

昆虫学报 ›› 2020, Vol. 63 ›› Issue (6): 717-726.doi: 10.16380/j.kcxb.2020.06.007

七星瓢虫触角转录组及嗅觉相关基因分析

杨雪娇^1,2, 张蔓^1,2, 江宇航^1,2, 唐启河^1,2, 张棋麟^1,2,*, 林连兵^1,2, 陈均远^3,4

(1. 昆明理工大学生命科学与技术学院, 昆明 650500; 2. 云南省高校饲用抗生素替代技术工程研究中心, 昆明 650500; 3. 中国科学院南京地质古生物研究所, 南京 210008; 4. 南京大学生命科学学院, 医药生物技术国家重点实验室, 南京 210023)

出版日期:2020-06-20 发布日期:2020-07-02

Analysis of the antennal transcriptome and olfaction-related genes of Coccinella septempunctata (Coleoptera: Coccinellidae)

YANG Xue-Jiao^1,2, ZHANG Man^1,2, JIANG Yu-Hang^1,2, TANG Qi-He^1,2, ZHANG Qi-Lin^1,2,*, LIN Lian-Bing^1,2, CHEN Jun-Yuan^3,4

(1. Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, China; 2. Engineering Research Center for Replacement Technology of Feed Antibiotics of Yunnan College, Kunming 650500, China; 3. Nanjing Institute of Geology and Paleontology, Chinese Academy of Sciences, Nanjing 210008, China; 4. State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210023, China)

Online:2020-06-20 Published:2020-07-02

摘要/Abstract

摘要： 【目的】瓢虫科食性高度分化。本研究旨在通过建立七星瓢虫Coccinella septempunctata触角转录组数据库，探讨其触角嗅觉相关基因与食性分化的关系。【方法】采用Illumina HiSeq 4000高通量测序平台对七星瓢虫成虫触角转录组进行测序、组装、注释，挖掘嗅觉相关基因，并与已发表的茄二十八星瓢虫Henosepilachna viginyioctopunctata转录组进行比较。【结果】共获得七星瓢虫触角转录组31 775条unigenes，其中69.71%的序列得以注释，NR数据库中注释最多，为20 539条。据注释信息，挖掘到27个嗅觉相关基因，包括1个气味结合蛋白(odorant binding protein, OBP)基因，13个化学感受蛋白(chemosensory protein, CSP)基因，4个气味受体(odorant receptor, OR)基因，7个味觉受体(gustatory receptor, GR)基因和2个感觉神经元膜蛋白(sensory neuron membrane protein, SNMP)基因。相对应地，在植食性茄二十八星瓢虫转录组中鉴定到38个嗅觉相关基因，包括七星瓢虫中未发现的1个离子型受体(ionotropic receptor, IR)基因。在各类型嗅觉相关基因中，茄二十八星瓢虫转录组的OBP基因比例(13.16%)高于七星瓢虫触角转录组的(3.70%)，而七星瓢虫触角转录组的GR基因比例(25.93%)则高于茄二十八星瓢虫转录组的(13.16%)。【结论】触角嗅觉相关基因数目不是昆虫食性分化的主因。本研究获得了七星瓢虫触角转录组学资源，初步探讨了嗅觉相关基因同瓢虫食性分化的关系，为了解瓢虫乃至昆虫食性分化的分子基础提供了信息。

关键词: 七星瓢虫, 触角转录组, 食性分化, 基因注释, 嗅觉相关基因

Abstract: 【Aim】 Coccinellidae show highly diverse feeding. This study aims to explore the relationships between olfaction-related genes in antennae and feeding divergence based on the establishment of the antennal transcriptome of Coccinella septempunctata. 【Methods】 The antennal transcriptome of C. septempunctata adults was sequenced using an Illumina HiSeq 4000 platform and subjected to assembly, annotation and comparative analysis with the previously published transcriptomes of Henosepilachna viginyioctopunctata, and the olfaction-related genes were identified. 【Results】 A total of 31 775 unigenes were obtained in C. septempunctata antennal transcriptome, 69.71% of which were annotated, and the highest number of unigenes (20 539) were annotated in the NR database. According to the annotation information, 27 olfaction-related genes including 1 odor binding protein (OBP) gene, 13 chemoreceptor protein (CSP) genes, 4 odorant receptor (OR) genes, 7 gustatory receptor (GR) genes and 2 sensory neuron membrane protein (SNMP) genes were identified. In parallel, 38 olfaction-related genes were identified in the transcriptomes of H. viginyioctopunctata, including 1 ionotropic receptor (IR) gene that was not found in C. septempunctata. Among the various types of olfaction-related genes, the proportion (13.16%) of OBP genes in the transcriptomes of H. viginyioctopunctata was higher than that (3.70%) in the antennal transcriptome of C. septempunctata, while the proportion (25.93%) of GR genes in the antennal transcriptome of C. septempunctata was higher than that (13.16%) in the transcriptomes of H. viginyioctopunctata. 【Conclusion】 The number of olfaction-related genes in the antennae may not make a predominant contribution to insect feeding divergence. This study obtained the antennal transcriptomic data of C. septempunctata and preliminarily explored the relationships between olfaction-related genes and feeding divergence in ladybirds, providing information for understanding the molecular basis of feeding divergence in ladybirds and even in insects.

Key words: Coccinella septempunctata, antennal transcriptome, feeding divergence, gene annotation, olfaction-related gene

杨雪娇, 张蔓, 江宇航, 唐启河, 张棋麟, 林连兵, 陈均远. 七星瓢虫触角转录组及嗅觉相关基因分析[J]. 昆虫学报, 2020, 63(6): 717-726.

YANG Xue-Jiao, ZHANG Man, JIANG Yu-Hang, TANG Qi-He, ZHANG Qi-Lin, LIN Lian-Bing, CHEN Jun-Yuan . Analysis of the antennal transcriptome and olfaction-related genes of Coccinella septempunctata (Coleoptera: Coccinellidae)[J]. Acta Entomologica Sinica, 2020, 63(6): 717-726.

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七星瓢虫触角转录组及嗅觉相关基因分析

Analysis of the antennal transcriptome and olfaction-related genes of Coccinella septempunctata (Coleoptera: Coccinellidae)

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