关键词: 东方绢金龟； 桃树挥发物, 行为反应； GC-EAD； 触角电位, Y型嗅觉仪

Abstract: 【Aim】 Maladera orientalis is an important garden pest in China. This study aims to identify peach tree volatile compounds attracting M. orientalis, so as to provide a theoretical basis for developing attractants of plant origin. 【Methods】 Leaf volatiles were collected from peach tree by dynamic headspace adsorption. The electrophysiologically active compounds for M. orientalis adults were identified from peach tree volatiles by gas chromatographymass spectrometry (GC-MS) and coupled gas chromatography electroantennogram detection (GC-EAD) techniques. The electrophysiological and behavioral responses of female adults of M. orientalis to the identified active compounds were tested by using electroantennography (EAG) and Y-tube olfactometer, respectively, using hexane as a control. 【Results】 Six active compounds were identified from peach tree volatiles, including E-2-hexenal, Z-3-hexenyl acetate, E-3-hexenol, Z-3-hexenol, methyl salicylate and an unknown compound. E-2-Hexenal triggered significantly greater EAG response in female adults of M. orientalis than the other four compounds at the doses of 1 and 10 μg. When the dose was increased to 100 μg, Z-3-hexenyl acetate triggered significantly lower EAG response in female adults of M. orientalis than the other four compounds. In behavioral response experiments, the female adults of M. orientalis were significantly attracted to E-3-hexenol and Z-3-hexenol at the dose of 100 μg. 【Conclusion】 Among peach tree volatile compounds, E-3-hexenol and Z-3-hexenol are significantly attractive to female adults of M. orientalis.

Key words: Maladera orientalis, peach tree volatiles, behavioral response, GC-EAD, electroantennogram, Y-tube olfactometer