家蚕鞣化激素基因对翅展及繁殖力的调控作用

doi:10.16380/j.kcxb.2021.05.002

昆虫学报 ›› 2021, Vol. 64 ›› Issue (5): 558-565.doi: 10.16380/j.kcxb.2021.05.002

家蚕鞣化激素基因对翅展及繁殖力的调控作用

杨佳鹏^#, 龙贵云^#, 金道超, 戴仁怀^*, 吴丽红, 周操, 杨熙彬

(贵州大学昆虫研究所, 贵州山地农业病虫害重点实验室, 农业农村部贵阳作物有害生物科学观测试验站, 贵阳 550025)

出版日期:2021-05-20 发布日期:2021-05-31

Role of Bursicon genes in regulating wing expansion and fecundity in Bombyx mori (Lepidoptera: Bombycidae)

YANG Jia-Peng^#, LONG Gui-Yun^#, JIN Dao-Chao, DAI Ren-Huai^*, WU Li-Hong, ZHOU Cao, YANG Xi-Bin

(Guizhou Provincial Key Laboratory for Agricultural Pest Management of the Mountainous Region, Scientific Observing and Experimental Station of Crop Pests in Guiyang, Ministry of Agriculture and Rural Affairs, Institute of Entomology, Guizhou University, Guiyang 550025, China)

Online:2021-05-20 Published:2021-05-31

摘要/Abstract

摘要：

【目的】昆虫鞣化激素(Bursicon)是由神经系统分泌的一种异源二聚体神经肽，对昆虫表皮鞣化、翅展等功能具有调控作用。本研究旨在探究鞣化激素基因与家蚕Bombyx mori翅发育及对繁殖力相关基因的关系，明确其对翅展和繁殖力的调控作用。【方法】采用RNAi技术，分别注射Bursicon基因的dsRNA(dsBmBurs-α, dsBmBurs-β和dsBmBurs-α+dsBmBurs-β)到家蚕1日龄蛹，以注射dsGFP为对照。观察RNAi后家蚕表型,并计算家蚕单雌产卵量;利用实时定量PCR技术检测Bursicon基因RNAi后家蚕羽化后24 h成虫中翅发育相关基因(BmWg, BmFt, BmFj和BmDs)和RNAi 48和72 h后家蚕蛹中繁殖相关基因(卵黄原蛋白基因BmVg和卵黄原蛋白受体基因BmVgR)的表达水平。【结果】家蚕Bursicon基因被RNAi后，羽化后24 h的家蚕翅不能正常伸展，dsBmBurs-α，dsBmBurs-β和dsBmBurs-α+dsBmBurs-β处理组的翅畸形率分别为93.33%, 96.67%和96.43%；平均单雌产卵量分别为312.67, 332.00和284.00粒，显著低于对照组(406.00粒)。RNAi干扰Bursicon基因后，家蚕Bursicon基因BmBurs-α和BmBurs-β表达量显著下调，其相关基因BmWg, BmFt, BmFj, BmDs, BmVg和BmVgR的表达水平均显著低于对照组。【结论】鞣化激素基因参与调节家蚕翅发育相关基因的转录水平来调控翅展。同时，它们还可以参与调节繁殖相关基因BmVg和BmVgR的表达，从而影响家蚕的繁殖。

关键词: 家蚕, 鞣化激素, 翅展, 繁殖力, Hippo信号通路

Abstract: 【Aim】 Bursicon secreted by nervous system is a heterodimer neuropeptide and plays an important role in regulating cuticle hardening and wing expansion of insects during molting cycle. This study aims to investigate the relationship between Bursicon genes and the genes involved in wing development and fecundity in Bombyx mori and to ascertain their role in regulating wing expansion and fecundity. 【Methods】 The RNAi technique was used to silence the Bursicon genes by injecting Bursicon dsRNA (dsBmBurs-α, dsBmBurs-β and dsBmBurs-α+dsBmBurs-β) into the 1-day-old pupae of B. mori, and the individuals injected with dsGFP were used as the control. After RNAi, the phenotype of B. mori was observed, and the number of eggs laid per female was calculated. The real-time quantitative PCR technology was used to detect the expression levels of wing development-related genes (BmWg, BmFt, BmFj and BmDs) in the 24 h-old adults of B. mori and reproduction-related genes (vitellogenin gene BmVg and vitellogenin receptor gene BmVgR) in pupae at 48 and 72 h after RNAi. 【Results】 After RNAi of Bursicon genes, the wings of the 24 hold adults of B. mori were not able to expand normally. In the three treatment groups injected with dsBmBurs-α, dsBmBurs-β and dsBmBurs-α+dsBmBurs-β, the wing deformity rates were 93.33%, 96.67% and 96.43%, respectively, and the average numbers of eggs laid per female were 312.67, 332.00 and 284.00, respectively, significantly lower than that of the control (406.00). After RNAi of Bursicon genes, the expression levels of Bursicon genes BmBurs-α and BmBurs-β in B. mori decreased significantly, and the expression levels of the related genes BmWg, BmFt, BmFj, BmDs, BmVg and BmVgR were significantly lower than those of the control group. 【Conclusion】 Bursicon genes are involved in regulating wing expansion by regulating the transcription levels of genes related to wing development in B. mori. They also regulate the expression of reproduction-related genes BmVg and BmVgR, and thus influence reproduction in B. mori.

Key words: Bombyx mori, Bursicon, wing expansion, fecundity, Hippo signaling pathway

杨佳鹏, 龙贵云, 金道超, 戴仁怀, 吴丽红, 周操, 杨熙彬 . 家蚕鞣化激素基因对翅展及繁殖力的调控作用[J]. 昆虫学报, 2021, 64(5): 558-565.

YANG Jia-Peng, LONG Gui-Yun, JIN Dao-Chao, DAI Ren-Huai, WU Li-Hong, ZHOU Cao, YANG Xi-Bin. Role of Bursicon genes in regulating wing expansion and fecundity in Bombyx mori (Lepidoptera: Bombycidae)[J]. Acta Entomologica Sinica, 2021, 64(5): 558-565.

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家蚕鞣化激素基因对翅展及繁殖力的调控作用

Role of Bursicon genes in regulating wing expansion and fecundity in Bombyx mori (Lepidoptera: Bombycidae)

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