昆虫学报 ›› 2021, Vol. 64 ›› Issue (9): 1080-1091.doi: 10.16380/j.kcxb.2021.09.008

• 研究论文 • 上一篇    下一篇

敲减神经肽F基因(npf)对亚洲玉米螟取食、生长发育和繁殖的影响

石坚#, 王原#, 梁佳, 杜娟, 赵章武*   

  1.  (中国农业大学植物保护学院昆虫系, 农业农村部作物有害生物监测与绿色防控重点实验室, 北京 100193)
  • 出版日期:2021-09-20 发布日期:2021-09-03

Effects of knocking down neuropeptide F gene (npf) on the feeding, growth and reproduction of Ostrinia furnacalis (Lepidoptera: Pyralididae)

SHI Jian#, WANG Yuan#, LIANG Jia, DU Juan, ZHAO Zhang-Wu*   

  1.  (Department of Entomology and MOA Key Laboratory of Pest Monitoring and Green Management, College of Plant Protection, China Agricultural University, Beijing 100193, China)
  • Online:2021-09-20 Published:2021-09-03

摘要:

【目的】神经肽F(neuropeptide F, NPF)是无脊椎动物特有的一类神经肽,因其C末端是苯丙氨酸(F)而命名,参与昆虫的取食、生物节律、学习记忆等多种生理功能的调控。本研究旨在明确NPF对亚洲玉米螟Ostrinia furnacalis生长发育的影响,为害虫防治提供重要依据。【方法】采用一种基于工程菌高效合成靶向昆虫基因的dsRNA的方法经济有效地敲降npf,用低浓度(0.01%)和高浓度(0.02%)dsNPF和dsGFP(对照)分别饲喂亚洲玉米螟1龄初、3龄初和5龄初幼虫直至化蛹,检测5龄幼虫平均取食量、体重、体长、存活率和化蛹率,蛹羽化率和成虫产卵量,以及幼虫各龄期、蛹发育历期和成虫寿命。【结果】从亚洲玉米螟1, 3和5龄初幼虫开始饲喂0.01%和0.02% dsNPF时,与饲喂相应浓度dsGFP的对照相比,除个别点外,5龄幼虫的取食量、体重、体长、存活率和化蛹率,蛹羽化率和成虫单雌产卵量均显著降低,幼虫各龄期、蛹发育历期均显著延长,成虫寿命显著缩短。且dsNPF处理幼虫的龄期越早对发育的影响越大。其中0.01% dsNPF处理的1龄幼虫和0.02% dsNPF处理的3龄幼虫有90%的个体在蛹期死亡,而0.02%dsNPF处理的1龄幼虫有90%的个体在幼虫期死亡。【结论】结果提示NPF对亚洲玉米螟的发育和取食具有调控作用,这为探索新型绿色的害虫防治提供了依据。

关键词: 亚洲玉米螟, 神经肽, NPF, 取食调控, 生长发育; 繁殖

Abstract: 【Aim】 The neuropeptide F (NPF), a type of neuropeptides in invertebrates named based on its C-terminal phenylalanine (F), participates in the regulation of many physiological functions in insects such as feeding, biological rhythm, learning and memory. The objective of this study is to clarify the effects of NPF on the growth and development of the Asian corn borer, Ostrinia furnacalis, so as to provide an important basis for pest control. 【Methods】 A method based on the efficient synthesis of dsRNA targeting genes by engineering bacteria was used to efficiently and economically knock down npf. Low concentration (0.01%) and high concentration (0.02%) of dsNPF and dsGFP (the control) were separately fed to the early 1st, 3rd and 5th instar larvae of O. furnacalis until pupation. The average feeding amount, body weight, body length, survival rate and pupation rate of the 5th instar larvae, as well as the emergence rate of pupae and the number of eggs laid by adults were detected. Besides, the developmental duration at different larval instars and pupal stage and adult life span were also detected. 【Results】 When the early 1st, 3rd and 5th instar larvae of O. furnacalis were fed with 0.01% and 0.02% dsNPF, the feeding amount, body weight, body length, survival rate and pupation rate of the 5th instar larvae, the emergence rate of pupae and the number of eggs laid per female adult significantly decreased compared with the control fed with the corresponding concentrations of dsGFP. The developmental duration of larvae and pupae in the treatment groups were significantly prolonged, and the life span of adults was significantly shorter. The earlier the larval instar was treated with dsNPF, the greater the impact on development. Among them, the 1st instar larvae treated with 0.01% dsNPF and the 3rd instar larvae treated with 0.02% dsNPF had 90% of their individuals died in the pupal stage, and the 1st instar larvae treated with 0.02% dsNPF had 90% of their individuals died in the larval stage. 【Conclusion】 The results suggest that NPF plays a regulation role in the development and feeding of O. furnacalis, providing a basis for the exploration of new green pest control.

Key words: Ostrinia furnacalis, neuropeptide, NPF, feeding regulation, growth and development, reproduction