昆虫学报 ›› 2021, Vol. 64 ›› Issue (10): 1176-1186.doi: 10.16380/j.kcxb.2021.10.007

• 研究论文 • 上一篇    下一篇

乙基多杀菌素多代胁迫对西花蓟马解毒酶活性、发育和繁殖的影响

李定银, 郅军锐*, 张涛, 岳文波   

  1. (贵州大学昆虫研究所, 贵州省山地农业病虫害重点实验室, 贵阳 550025)
  • 出版日期:2021-10-20 发布日期:2021-09-22

Effects of multigenerational spinetoram stress on the detoxification enzyme activities, development and reproduction of Frankliniella occidentalis (Thysanoptera: Thripidae)

LI Ding-Yin, ZHI Jun-Rui*, ZHANG Tao, YUE Wen-Bo   

  1.  (Guizhou Provincial Key Laboratory for Agricultural Pest Management of the Mountainous Region, Institute of Entomology, Guizhou University, Guiyang 550025, China)
  • Online:2021-10-20 Published:2021-09-22

摘要:

【目的】乙基多杀菌素是防治蔬菜上西花蓟马Frankliniella occidentalis的首选药剂之一。本研究旨在明确乙基多杀菌素多代胁迫对西花蓟马的影响。【方法】采用浸叶法以LC25浓度的乙基多杀菌素对西花蓟马2龄若虫连续汰选6代后,测定其抗药性和解毒酶活性的变化,并用年龄-阶段两性生命表分析LC25浓度乙基多杀菌素对西花蓟马后代(F7)生命表参数的影响。【结果】LC25浓度的乙基多杀菌素对西花蓟马2龄若虫连续汰选6代后,与用清水处理的对照相比,抗性倍数达到了6.4倍;F6代2龄第2天若虫体内羧酸酯酶(CarE)和谷胱甘肽-S转移酶(GSTs)被激活,活性分别增加至对照的1.26和1.21倍;而多功能氧化酶(MFOs)和细胞色素P450酶(P450s)受到明显的抑制,活性分别只有对照的54.00%和51.78%。LC25浓度的乙基多杀菌素胁迫6代不仅造成西花蓟马雌成虫寿命(19.33 d)和单雌产卵量(69.80粒)较对照(分别为21.60 d和83.17粒)显著下降,且后代(F7)的雌成虫寿命(16.82 d)和雄成虫寿命(8.29 d)较对照(分别为20.28 d和10.86 d)极显著缩短,单雌产卵量(59.23粒)较对照(76.96粒)极显著降低,但雌雄比(2.28)较对照(1.03)显著提高,种群生命参数净增殖率(R0)、内禀增长率(r)和周限增长率(λ)则无明显变化。【结论】在LC25浓度乙基多杀菌素连续汰选6代内西花蓟马对该药剂的抗性缓慢增长;药剂胁迫不仅对西花蓟马解毒酶活性有不同程度的影响,还对其发育和繁殖具有一定的抑制作用。

关键词:  西花蓟马, 乙基多杀菌素, 解毒酶, 杀虫剂汰选, 抗药性

Abstract:

【Aim】 Spinetoram is one of the candidate pesticides most used for controlling the western flower thrips, Frankliniella occidentalis, on vegetables. This study aims to assess the effects of multigeneration spinetoram stress on F. occidentalis. 【Methods】 The 2nd instar nymphs of F. occidentalis were screened for six generations with LC25 dosage of spinetoram by the leaf dipping method, and the insecticide sensitivity and detoxification enzyme activities in the nymphs were determined. The agestage twosex life table was used to analyze the effect of LC25 dosage of spinetoram on the life table parameters of F. occidentalis offspring (F7). 【Results】 After the 2nd instar nymphs of F. occidentalis were selected with LC25 dosage of spinetoram for six generations, the resistance ratio increased to 6.4 folds as high as that of the control treated with water. CarE and GSTs in the day-2 2nd instar nymphs of F6 generation were significantly activated, with their activities 1.26 and 1.21 folds as high as those of the control, while MFOs and P450s were significantly inhibited, with their activities only 54.00% and 51.78% of those of the control, respectively. When the 2nd instar nymphs of F. occidentalis were selected with LC25 dosage of spinetoram for six generations, the longevity (19.33 d) and fecundity (69.80 eggs) of female adult significantly decreased as compared to those of the control (21.60 d and 83.17 eggs, respectively), the female adult longevity (16.82 d) and male adult longevity (8.29 d) of F7 generation were also significantly shortened as compared to those of the control (20.28 d and 10.86 d, respectively), the fecundity of F7 generation (59.23 eggs laid per female) extremely significantly decreased as compared to that of the control (76.96 eggs laid per female), but the female to male sex ratio (2.28) significantly increased as compared to that of the control (1.03). The net reproductive rate (R0), intrinsic rate of increase (r) and finite rate of increase (λ) in F7 generation of F. occidentalis after selection with LC25 dosage of spinetoram for six generations had no significant change. 【Conclusion】 The resistance of F. occidentalis to spinetoram

increases slowly within six generations under selection by LC25 dosage of spinetoram. Spinetoram stress not only causes significant changes in the detoxification enzyme ctivities in F. occidentalis, but also inhibits its development and reproduction.

Key words: Frankliniella occidentalis, spinetoram, detoxification enzyme, insecticide screening, insecticide resistance