昆虫学报 ›› 2021, Vol. 64 ›› Issue (12): 1465-1477.doi: 10.16380/j.kcxb.2021.12.012

• 综 述 • 上一篇    下一篇

鳞翅目昆虫内共生菌研究进展

刘媛1,2, 王浩1,2, 王志鹏1,2, 陈利民1,2, 王亚如1,2, 侯有明1,2,*   

  1. (1. 福建农林大学植物保护学院, 闽台作物有害生物生态防控国家重点实验室, 生物农药与化学生物学教育部重点实验室, 福州 350002; 2. 福建省昆虫生态重点实验室, 福州 350002)
  • 出版日期:2021-12-20 发布日期:2021-11-26

Research progress of endosymbionts in lepidopteran insects

LIU Yuan1,2, WANG Hao1,2, WANG Zhi-Peng1,2, CHEN Li-Min1,2, WANG Ya-Ru1,2, HOU You-Ming1,2,*   

  1. (1.State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, College of Plant Protection, Fujian Agricultural and Forestry University, Fuzhou 350002, China; 2. Fujian Province Key Laboratory of Insect Ecology, Fuzhou 350002, China)
  • Online:2021-12-20 Published:2021-11-26

摘要:

 内共生菌(endosymbionts)与其昆虫宿主的共生关系是普遍存在的,它们彼此相互依赖、相互影响、协同进化。近年来,关于昆虫内共生菌的研究多以半翅目(Hemiptera)和双翅目(Diptera)昆虫为主,但数量不断增加的研究表明鳞翅目(Lepidoptera)昆虫与其体内共生菌的互作模式和机制也正在受到越来越多的关注。鳞翅目昆虫种类多,分布广,主要作为植食者、传粉者在生态系统中发挥作用,而其绝大部分幼虫会对农林业生产造成巨大经济损失。鳞翅目昆虫体内共生菌群落多样性相对较低,主要以次生共生菌Wolbachia为主,少数也感染有SpiroplasmaArsenophonusRickettsia。它们常呈严格的母系垂直传播,也会发生一定比例的水平传播,在宿主的生长发育、生殖调控、环境适应、遗传进化方面发挥重要作用。目前一般采用诊断性聚合酶链反应、高通量扩增子测序、宏基因组测序等方法检测内共生菌。但鳞翅目昆虫内共生菌研究领域存在一些难点,包括:大多数内共生菌无法离体培养;丰度较低的内共生菌的生物学功能难以确定。基于鳞翅目昆虫内共生菌的分布及该领域的难点,建议未来的研究重点应放在次生共生菌及其生物学功能上。

关键词: 鳞翅目, 内共生菌; Wolbachia, Spiroplasma, 传播方式, 生物学效应, 共生关系

Abstract: The symbiotic relationship between endosymbionts and their insect hosts is ubiquitous in nature, and they are interdependent, interacting and coevolving. In recent years, studies on insect endosymbionts mainly focus on hemipteran and dipteran insects. However, a growing number of studies show that the interaction mode and mechanism between lepidopteran insects and their endosymbionts are also attracting more and more attention. Lepidopteran insects are widely distributed and play important roles in the ecosystem as herbivores and pollinators, and most of their larvae can cause great economic losses to the agricultural and forestry production. The diversity of endosymbiont community in lepidopteran insects is relatively low, which is mainly dominated by secondary symbiont Wolbachia. A few species are also infected with Spiroplasma, Arsenophonus and Rickettsia. These endosymbionts are mainly transmitted maternally from mother to offspring, whereas their horizontal transmission may also be occurring in nature. And they play important roles in the growth and development, reproductive manipulation, environmental adaptation and genetic evolution of hosts. Nowadays, diagnostic polymerase chain reaction (PCR), high-throughput amplicon sequencing, and metagenomic sequencing are generally used to detect endosymbionts. However, there are still some difficulties in the research of endosymbionts in lepidopteran insects, including that most endosymbionts can not be cultured in vitro, and the biological functions of endosymbionts with low abundance are difficult to be determined. Considering the distribution of endosymbionts and difficulties in lepidopteran insects, it is suggested that future research should focus on secondary symbionts and their biological functions.

Key words:  Lepidoptera, endosymbiont; Wolbachia, Spiroplasma, transmission mode, biological effect, symbiotic relationship