昆虫学报 ›› 2022, Vol. 65 ›› Issue (8): 986-998.doi: 10.16380/j.kcxb.2022.08.007

• 研究论文 • 上一篇    下一篇

不同饲料饲养的近暗散白蚁工蚁肠道中差异蛋白分析

苏丽娟1, 赵鹏飞2, 杜运良1, 关宇梁1, 郭瑞瑶1, 阴正兴3, 宋安东1,*   

  1. (1. 河南农业大学生命科学学院, 郑州 450002; 2. 大坝(河南)工程技术有限公司, 郑州 450018; 3. 河南牧业经济学院, 郑州 450046)
  • 出版日期:2022-08-20 发布日期:2022-09-16

Analysis of differential proteins in Reticulitermes perilucifugus (Isoptera: Rhinotermitidae) workers fed with different diets

SU Li-Juan1, ZHAO Peng-Fei2, DU Yun-Liang1, GUAN Yu-Liang1, GUO Rui-Yao1, YIN Zheng-Xing3, SONG An-Dong1,*    

  1. (1. Henan Agricultural University, College of Life Sciences, Zhengzhou 450002, China; 2. Dam (Henan) Engineering Technology Co., Ltd, Zhengzhou 450018, China; 3. Henan University of Animal Husbandry and Economy, Zhengzhou 450046, China)
  • Online:2022-08-20 Published:2022-09-16

摘要: 【目的】本研究旨在通过比较不同木质纤维素含量的饲料对近暗散白蚁Reticulitermes perilucifugus工蚁前中肠和后肠内容物蛋白的组成和表达差异,为揭示白蚁的营养消化吸收机理提供蛋白水平上的依据。【方法】用木质纤维素含量不同的3种饲料(松木、秸秆和滤纸)饲养近暗散白蚁工蚁,然后用双向电泳分析肠道不同部位的内容物蛋白,并对差异蛋白进行MALDI-TOF/MS测序及生物信息学分析。【结果】双向电泳结果发现,同一饲料饲养的近暗散白蚁工蚁前中肠可辨蛋白点数明显多于后肠;不同饲料饲喂的工蚁前中肠和后肠可辨蛋白点数依次是松木饲养的>滤纸饲养的>秸秆饲养的。通过对115个蛋白点的测序分析表明,不同饲料饲喂的工蚁前中肠和后肠中的主要差异蛋白为具有催化活性的蛋白质,包括与氨基酸代谢、丙酮酸代谢、碳代谢、氮代谢、TCA循环、糖酵解、糖异生和纤维素降解等相关的酶,以及参与细胞组成和信号传导的蛋白。【结论】木质纤维素含量不同的饲料饲养的近暗散白蚁工蚁肠道中的差异蛋白主要为具有催化活性的蛋白、细胞组成蛋白和信号传导蛋白,表明不同的饲料影响近暗散白蚁的肠道蛋白组成。这些结果为揭示白蚁对木质纤维素的降解机理提供了参考。

关键词: 近暗散白蚁, 木质纤维素, 双向电泳, 肠道内容物, MALDI-TOF/TOF

Abstract: 【Aim】 This study aims to provide the basis at the protein level for exploring the mechanism of nutrient digestion and absorption in termites by comparing the differences in protein composition and expression in the fore-midgut and hindgut contents of Reticulitermes perilucifugus workers fed with diets with different lignocellulose contents. 【Methods】 R. perilucifugus workers were fed with three diets (pine, straw and filter paper) with different lignocellulose contents. The proteins in contents of different parts of their gut were analyzed by two dimensional (2D) electrophoresis, and the differiential proteins were sequenced and bioinformatically analyzed by MALDI-TOF/MS. 【Results】 The results of 2D electrophoresis showed that the number of visible protein spots in the fore-midgut of R. perilucifugus workers fed with the same diet was significantly higher than that in the hindgut, while those in the fore-midgut and hindgut of workers fed with pine were the highest, those in the fore-midgut and hindgut of workers fed with filter paper followed, and those in the fore-midgut and hindgut of workers fed with straw were the least. The sequencing results of 115 protein points showed that the mainly differential proteins between the fore-midgut and hindgut were proteins with catalytic activity including enzymes related to amino acid metabolism, pyruvate metabolism, carbon metabolism, nitrogen metabolism, TCA cycle, glycolysis, gluconeogenesis and cellulose degradation and proteins involved in cellular components and signal transduction. 【Conclusion】 The differential proteins in the gut of R. perilucifugus workers fed with different diets are 
mainly proteins with catalytic activity, and proteins involved in cell composnents and signal transduction, indicating that different diets affect the composition of intestinal proteins of R. perilucifugus. These results provide data for revealing the degradation mechanism of lignocellulose in termites.

Key words: Reticulitermes perilucifugus; lignocellulose, two dimensional electrophoresis; intestinal contents, MALDI-TOF/TOF