昆虫学报 ›› 2023, Vol. 66 ›› Issue (2): 226-234.doi: 10.16380/j.kcxb.2023.02.012

• 研究论文 • 上一篇    下一篇

冷藏温度对日本刀角瓢虫存活及生长发育的影响

曹惠怡1,罗小龙1,韦雪媛1,梁建锋1,谢永辉2,*,王兴民1,*   

  1. (1. 华南农业大学植物保护学院昆虫学系, 广东省生物农药创制与应用重点实验室, 广东省农业害虫生物防治工程研究中心,
    广州 510642; 2. 云南省烟草公司昆明市公司, 昆明 650201)
  • 出版日期:2023-02-20 发布日期:2023-04-07

Effects of cold-storage temperature on the survival and growth and development of Serangium japanicum(Coleoptera: Coccinellidae)

CAO Hui-Yi1, LUO Xiao-Long1, WEI Xue-Yuan1, LIANG Jian-Feng1, XIE Yong-Hui2,*, WANG Xing-Min1,*   

  1. (1. Engineering Research Center of Biological Control of Agricultural Pests of Guangdong Province, Key Laboratory of BioPesticide innovation and Application of Guangdong Province, Department of Entomology, College of Plant Protection, South China Agricultural University, Guangzhou 510642, China; 2. Yunnan Tobacco Company Kunming Branch, Kunming 650201, China)
  • Online:2023-02-20 Published:2023-04-07

摘要: 【目的】本研究旨在调查不同冷藏温度下日本刀角瓢虫Serangium japanicum成虫生物学特性及F1代的生长发育,明确日本刀角瓢虫成虫最适冷藏温度。【方法】将日本刀角瓢虫成虫置于不同低温(7,10,13和16 ℃), 贮藏10 d时测定其存活率、单雌产卵量、寿命和对烟粉虱Bemisia tabaci 4龄若虫的日捕食量,以及日本刀角瓢虫F1代存活率和发育历期;qRT-PCR测定日本刀角瓢虫成虫体内热激蛋白基因Hsp70和Hsp90的相对表达量。【结果】日本刀角瓢虫成虫在16 ℃低温贮藏10 d时,其存活率、雌雄成虫寿命、单雌产卵量和日捕食量以及F1代存活率均与贮藏在26 ℃的对照无显著差异(存活率: 99% vs 100%; 雌成虫寿命: 110.65 d vs 106.87 d; 雄成虫寿命: 12312 d vs 108.79 d; 单雌产卵量: 399.19粒vs 422.63粒; 日捕食量: 34.70头vs 31.95头; F1代存活率: 80.39% vs 94.12%);但其F1代发育历期与对照相比显著缩短(17.33 d vs 18.89 d)。7 ℃贮藏10 d时,日本刀角瓢虫成虫全部死亡;10 ℃贮藏时,其存活率、单雌产卵量和F1代存活率受到显著影响;13 ℃贮藏10 d时单雌产卵量受到显著影响。各贮藏低温均能诱导日本刀角瓢虫Hsp70和Hsp90的表达量上调,且温度越低,相对表达量越高;10 ℃处理组成虫体内Hsp70和Hsp90的相对表达量分别为对照的7.06和2.33倍。【结论】综合以上研究结果,日本刀角瓢虫最适冷藏温度为16 ℃,热激蛋白Hsp70和 Hsp90可能在日本刀角瓢虫成虫响应低温胁迫时发挥了保护作用。

关键词: 日本刀角瓢虫;低温贮藏;生物学特性;存活, 生长发育;热激蛋白

Abstract: 【Aim】 This study aims to investigate the biological characteristics of adults and the growth and development of F1 generation of Serangium japonicum at different cold-storage temperatures, so as to determine the optimal cold-storage temperature of S. japonicum adults.【Methods】 S. japonicum adults were stored at different low temperatures (7, 10, 13 and 16 ℃), their survival rates, numbers of eggs laid per female, longevity and daily predation amount against the 4th instar nymphs of Bemisia tabaci, the survival rates and developmental duration of F1 generation of S. japonicum, and the relative expression levels of the heat shock protein genes Hsp70 and Hsp90 in S. japonicum adults were determined at 10 d after storage by qRT-PCR. 【Results】At 10 d after S. japonicum adults were stored at 16 ℃, their survival rate, female and male adult longevity, number of eggs laid per female and daily predation amount, and the survival rate of F1 generation were not significantly different from those of the control stored at 26 ℃ (survival rate: 99% vs 100%; female adult longevity: 110.65 d vs 106.87 d; male adult longevity: 123.12 d vs 108.79 d; number of eggs laid per female: 399.19 grains vs 422.63 grains; daily predation amount: 34.70 individuals vs 31.95 individuals; F1 progeny survival rate: 8039% vs 94.12%); but the developmental duration of F1 generation was significantly shorter than that of the control (17.33 d vs 18.89 d). At 10 d after coldstorage, all S. japonicum adults died when stored at 7 ℃, when stored at 10 ℃, their survival rate and number of eggs laid per female and the survival rate of F1 generation were significantly affected, and when stored at 13 ℃, the number of eggs laid per female was significantly affected. Various cold-storage temperatures induced the up-regulation of the expression levels of both Hsp70 and Hsp90 in S. japonicum adults at 10 d after storage, and the lower the temperature, the higher the relative expression levels. The relative expression levels of Hsp70 and Hsp90 in the treatment group of 10 ℃ were 7.06- and 2.33-fold, respectively, as high as those in the control.【Conclusion】 Combining the above results, the optimum cold-storage temperature for S. japonicum adults is 16 ℃, and the heat shock proteins Hsp70 and Hsp90 may play a protective role in the response to cold stress in S. japonicum adults.

Key words: Serangium japonicum, cold-storage, biological characteristics, survival, growth and development, heat shock protein