昆虫学报 ›› 2024, Vol. 67 ›› Issue (1): 9-17.doi: 10.16380/j.kcxb.2024.01.002

• 研究论文 • 上一篇    下一篇

色素通路相关基因在红背和黑背中华蜜蜂成年工蜂背板中的表达模式分析

王若虹1, #, 杨振慧1, #, 周诗文1, 吴雨珈1, 李秋方1,2, 梁立强1, 石丹丹1, 杨尚宁1, 苗刘畅1, 苏松坤1, *, 聂红毅1, *   

  1. (1. 福建农林大学蜂学与生物医药学院, 福州 350002; 2. 恩施土家族苗族自治州农业科学院, 恩施445000)

  • 出版日期:2024-01-20 发布日期:2024-01-27

Analysis of expression patterns of genes involved in pigment pathways in the tergum of the red- and black-backed Apis cerana cerana adult workers

WANG Ruo-Hong1, #, YANG Zhen-Hui1, #, ZHOU Shi-Wen1, WU Yu-Jia1, LI Qiu-Fang1, 2, LIANG Li-Qiang1, SHI Dan-Dan1, YANG Shang-Ning1, MIAO Liu-Chang1, SU Song-Kun1,*, NIE Hong-Yi1,*
  

  1.  (1. College of Bee Science and Biomedicine),FujianAgriculture andForestryUniversity,Fuzhou350002,China; 2. Enshi Tujia and Miao AutonomousPrefectureAcademyof Agricultural Sciences, Enshi 445000,China)
  • Online:2024-01-20 Published:2024-01-27

摘要:

【目的】分析红背中华蜜蜂Apis cerana cerana色素通路相关基因的表达差异,揭示红背中华蜜蜂色素形成的分子机制。【方法】采用体视显微镜观察刚出房红背中华蜜蜂和黑背中华蜜蜂(正常个体)成年工蜂的体色差异。通过同源比对鉴定中华蜜蜂成年工蜂黑色素代谢通路相关8个基因(PAH, TH, DDC, ebony, tan, aaNAT, yellow-ylaccase 2)、蝶呤通路相关4个基因(GTPCH I, SPR, PTPSGC-1)、眼色素通路相关2个基因(vermilioncinnabar)以及尿酸盐转运相关4个基因(BLOS2, HPS5, OKVarp)的同源基因;运用荧光定量PCR检测上述色素通路相关基因在红背中华蜜蜂和黑背中华蜜蜂成年工蜂胸部背板和腹部体壁中的相对表达量。【结果】红背中华蜜蜂和黑背中华蜜蜂成年工蜂体色差异表现在胸部背板:红背中华蜜蜂胸部背板呈现棕红色,黑背中华蜜蜂胸部背板则为黑色。荧光定量PCR检测结果表明,tan, laccase 2, SPR, vermilion, cinnabar, BLOS2OK以及OK表达量分别在红背中华蜜蜂成年工蜂与黑背中华蜜蜂成年工蜂间的胸部背板和腹部体壁中有显著性差异。【结论】红背中华蜜蜂和黑背中华蜜蜂明显的体色差异位于胸部背板,这种体色分化的现象受到蜜蜂体内黑色素、蝶呤、眼色素通路及尿酸盐转运相关基因共同作用的影响。

关键词:  红背中华蜜蜂, 形态观察, 色素通路, 胸部背板, 荧光定量PCR

Abstract:


【Aim】To analyze the differential expression of genes involved in the pigment pathway of red-backed Apis cerana cerana and reveal the molecular mechanism of pigment formation of red-backed A. cerana cerana. 【Methods】 The differences in the adult worker body color between the newly emerged red- and black-backed (normal individuals) A. cerana cerana were observed using stereomicroscope. Homologous genes of 8 genes related to melanin metabolism pathway (PAHTHDDCebonytanaaNATyellow-y and laccase 2), 4 genes related to pterin pathway (GTPCH ISPRPTPS and GC-1), 2 genes related to ommochrome pigment pathway (vermilion and cinnabar), and 4 genes related to urate transport protein (BLOS2HPS5OK and Varp) were identified in adult A. cerana cerana workers via BLAST. The relative expression levels of the above genes involved in pigment pathways in the thoracic tergum and abdominal integument of the red- and black-backed A. cerana cerana adult workers were detected by fluorescence quantitative PCR. 【Results】 The color difference in the adult worker body color between the red- and black-backed A. cerana cerana was found on thoracic tergum. The thoracic tergum of the red-backed A. cerana cerana is in brownish red, while that of the black-backed A. cerana cerana is in black. The fluorescence quantitative PCR results showed that the expression levels of tanlaccase 2, SPRvermilioncinnabarBLOS2 and OK and that of OK in the thoracic tergum and abdominal integument had significant difference, respectively, between the red-backed adult A. cerana cerana workers and black-backed adult A. cerana cerana workers. 【Conclusion】 Red-backed A. cerana cerana and black-backed A. cerana cerana have obvious body color difference on the thoracic tergum. This phenomenon of body color differentiation is influenced by the combined effects of genes related to melanin, pterin and ommochrome pigment pathways, and urate transport in honeybee.


Key words: Red-backed Apis cerana cerana, morphological observation, pigment pathway, thoracic tergum, fluorescence quantitative PCR