稻纵卷叶螟对氯虫苯甲酰胺的抗性监测及其抗氯虫苯甲酰胺种群对其他双酰胺类杀虫剂的交互抗性

doi:10.16380/j.kcxb.2024.04.006

昆虫学报 ›› 2024, Vol. 67 ›› Issue (4): 498-506.doi: 10.16380/j.kcxb.2024.04.006

稻纵卷叶螟对氯虫苯甲酰胺的抗性监测及其抗氯虫苯甲酰胺种群对其他双酰胺类杀虫剂的交互抗性

王立¹, 董蓓蓓¹, 刘思彤^1，2, 陈韵箫¹, 杨凤霞¹, 张帅³, 高聪芬^1,*

(1. 南京农业大学植物保护学院, 绿色农药创制与应用技术国家地方联合工程研究中心, 南京 210095; 2. 云南农垦产业研究院有限公司, 昆明 650106; 3. 全国农业技术推广服务中心, 北京 100026)

出版日期:2024-04-20 发布日期:2024-05-24

Monitoring of chlorantraniliprole resistance in the rice leaffolder, Cnaphalocrocis medinalis (Lepidoptera: Pyralidae) and the cross-resistance of its chlorantraniliprole-resistant populations to other diamide insecticides

WANG Li¹, DONG Bei-Bei¹, LIU Si-Tong^{1, 2}, CHEN Yun-Xiao¹, YANG Feng-Xia¹, ZHANG Shuai³, GAO Cong-Fen^1,*

(1. State & Local Joint Engineering Research Center of Green Pesticide Invention and Application, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China; 2. Yunnan Agricultural Reclamation Industry Research Institute Co., Ltd., Kunming 650106, China; 3. National Agro-Tech Extension and Service Center, Beijing 100026, China)

Online:2024-04-20 Published:2024-05-24

摘要/Abstract

摘要： 【目的】本研究旨在明确稻纵卷叶螟Cnaphalocrocis medinalis对氯虫苯甲酰胺的抗性水平、抗性稳定性及其抗氯虫苯甲酰胺种群对其他双酰胺类杀虫剂是否存在交互抗性。【方法】采用稻苗浸渍法测定我国9省（自治区）稻纵卷叶螟32个田间种群对氯虫苯甲酰胺和四唑虫酰胺的抗性，并测定了两个氯虫苯甲酰胺抗性种群对其他双酰胺类杀虫剂的交互抗性，以及湖南攸县、安徽潜山、安徽庐江和广西兴安田间种群在室内不接触药剂饲养后对氯虫苯甲酰胺的抗性稳定性。【结果】 2019-2022年间，稻纵卷叶螟田间种群对氯虫苯甲酰胺的抗性逐年上升，2022年首次监测到庐江、兴安、武穴、丹阳和潜山种群对氯虫苯甲酰胺达到高水平抗性(102.3~135.1倍)，其他种群亦达到中等水平抗性(10.3~97.1倍)； 2019-2021年监测的所有田间种群对四唑虫酰胺均表现为敏感，2022年所监测种群对四唑虫酰胺达到中等水平抗性(41.9~98.0倍)。交互抗性试验结果表明，对氯虫苯甲酰胺抗性约100倍的浙江嘉兴和安徽潜山种群对氟氯虫双酰胺、四唑虫酰胺、溴氰虫酰胺、环丙虫酰胺和四氯虫酰胺也表现出31.6~100.5倍中等至高水平抗性。稻纵卷叶螟田间种群在室内不接触药剂连续饲养2~4代后，对氯虫苯甲酰胺的抗性表现出快速下降的现象。【结论】稻纵卷叶螟田间种群对氯虫苯甲酰胺和四唑虫酰胺已产生中等至高水平抗性，且对氯虫苯甲酰胺产生抗性的稻纵卷叶螟田间种群对其他5种双酰胺类药剂也存在中等至高水平的交互抗性；鉴于当前稻纵卷叶螟田间种群对氯虫苯甲酰胺的抗性尚不稳定，建议可通过限制或暂停使用该类药剂，以延缓其抗性发展和延长使用寿命。

关键词: 稻纵卷叶螟, 抗药性监测, 氯虫苯甲酰胺, 交互抗性, 抗性稳定性

Abstract: 【Aim】 The objective of this research is to clarify the resistance level and resistance stability of chlorantraniliprole in the rice leaffolder, Cnaphalocrocis medinalis, and to determine whether there are cross-resistance to other diamide insecticides in chlorantraniliprole-resistant populations. 【Methods】 The rice seedling dipping method was adopted to determine the resistance of 32 field populations of C. medinalis collected from nine provinces (autonomous regions) to chlorantraniliprole and tetraniliprole, the cross-resistance to other diamide insecticides in two chlorantranilipeole-resistant populations, and the resistance stability when the populations from Youxian, Hunan, Qianshan, Anhui, Lujiang, Anhui and Xing′an,Guangxi were not exposed to chlorantraniliprole in laboratory. 【Results】 The resistance of chlorantraniliprole in C. medinalis field populations kept increasing during 2019-2022. Lujiang, Xing′an, Wuxue, Danyang and Qianshan populations were monitored for the first time to have developed high level of resistance to chlorantraniliprole (102.3-135.1-fold), and other populations also reached moderate level of resistance (10.3-97.1-fold) in 2022. To tetraniliprole, all the monitored field populations kept susceptible during 2019-2021, while the populations monitored in 2022 have developed moderate level of resistance (41.9-98.0-fold). Moreover, the cross-resistance experiment results revealed that Jiaxing and Qianshan populations which appeared about 100-fold resistance to chlorantraniliprole also showed 31.6-100.5-fold cross-resistance to cyhalodiamide, tetraniliprole, cyantraniliprole, cyclaniliprole and tetrachlorantraniliprole. In addition, the chlorantraniliprole resistance of field populations decreased rapidly after 2-4 generations of successive rearing without exposure to insecticides in laboratory. 【Conclusion】 The field populations of C. medinalis have developed moderate to high level of resistance to chlorantraniliprole and tetraniliprole. Moderate to high level of cross-resistance to five other diamide insecticides was found in chlorantraniliprole-resistant populations. Besides, the resistance to chlorantraniliprole was unstable in C. medinalis field populations. So, we strongly suggested limiting or suspending the application of diamide insecticides in order to delay the resistance development and postpone the application of diamide insecticides in C. medinalis control in the future.

Key words: Cnaphalocrocis medinalis, resistance monitoring, chlorantraniliprole, cross-resistance, resistance stability

王立, 董蓓蓓, 刘思彤, 陈韵箫, 杨凤霞, 张帅, 高聪芬. 稻纵卷叶螟对氯虫苯甲酰胺的抗性监测及其抗氯虫苯甲酰胺种群对其他双酰胺类杀虫剂的交互抗性[J]. 昆虫学报, 2024, 67(4): 498-506.

WANG Li, DONG Bei-Bei, LIU Si-Tong, CHEN Yun-Xiao, YANG Feng-Xia, ZHANG Shuai, GAO Cong-Fen . Monitoring of chlorantraniliprole resistance in the rice leaffolder, Cnaphalocrocis medinalis (Lepidoptera: Pyralidae) and the cross-resistance of its chlorantraniliprole-resistant populations to other diamide insecticides[J]. Acta Entomologica Sinica, 2024, 67(4): 498-506.

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稻纵卷叶螟对氯虫苯甲酰胺的抗性监测及其抗氯虫苯甲酰胺种群对其他双酰胺类杀虫剂的交互抗性

Monitoring of chlorantraniliprole resistance in the rice leaffolder, Cnaphalocrocis medinalis (Lepidoptera: Pyralidae) and the cross-resistance of its chlorantraniliprole-resistant populations to other diamide insecticides

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