›› 1980, Vol. 23 ›› Issue (3): 233-241.doi:

• 论文 •    下一篇

昆虫线粒体发生的生化和亚显微结构的研究

刘树森, 蔡惠罗, 徐慕禹   

  1. 中国科学院动物研究所细胞生物学室
  • 出版日期:1980-07-15 发布日期:1980-07-15

BIOCHEMICAL AND STRUCTURAL STUDIES ON THE BIOGENESIS OF THE MITOCHONDRIA IN DEVELOPING FLIGHT MUSCLE OF INSECTS

LIU SHU-SEN, TSAI HUI-LO, HSU MU-YU   

  1. Department of Cell Biology, Institute of Zoology, Academia Sinica
  • Online:1980-07-15 Published:1980-07-15

摘要: 线粒体在细胞中的发生目前有各种观点的争论,其理论意义涉及到真核细胞的起源和进化、染色体和线粒体两个遗传体系之间的相互关系以及生物膜合成和组装机理等。我们对处于分化中的昆虫胸肌线粒体的观察结果是:(1)对粘虫变态期的呼吸和细胞色素氧化酶活力测定表明蛹期第8天的组织形成阶段是胸肌细胞分化和其线粒体发生的开始。电镜观察表明,线粒体形成分两个阶段:由颗粒结构(可能是酶蛋白与脂的复合体)装配成膜片和膜泡;由膜泡分化出内嵴,进而发育为线粒体。(2)QO2值,P/O比和ATP酶活力的出现与膜结构的分化发育相平行。α-甘油磷酸氧化酶系统比谷氨酸氧化酶系统装配早;电子传递酶系比磷酸化酶系装配早。(3)蝗虫胸肌分化过程的电镜观察证明;先形成内膜小泡(直径约0.1微米左右),后形成外膜,组成简单线粒体;后者进一步分化发育为成熟线粒体。(4)QO2值,P/O比和ATP酶活力与膜结构分化发育相平行。ATP酶的出现与能量转涣功能呈平行关系。膜形成早期和“幼稚”线粒体阶段,ATP酶尚未装配。(5)综合上述结果:线粒体膜由非膜结构逐步组装形成,线粒体内膜的各酶系组装次序不同步,线粒体DNA控制合成的膜蛋白在膜结构形成中似乎起核心和骨架作用;线粒体总组装过程在不同细胞中表现为多种途径和方式。

Abstract: At present it is well known that the mitochondria are formed by a close intereaction of two distinct genctic systems: the nucleo-cytoplasmic system and the cell are assembled.The present investigation provides some evidences indicating that during the process of mitochondria biogenesis in armyworm species the outer mitochondreal membrane is formed first,followed by the inner mitochondrial membrane and crestae system; but in locust species the mitochodria biogenesis process begins with the formation of inner mitochondrial membrane vesicles followed by the outer mitochondrial membrane.The experimental results obtained from these two insects are summarized as follows:I. Electronmicroscopic examinations (Plates I:1-4, II:5-6) indicated that their mitochondria of armyworm are formed from non-membraneous precusors (macromalecute particles).