›› 1993, Vol. 36 ›› Issue (2): 167-171.

• 研究论文 • 上一篇    下一篇

阿特拉津和敌敌畏对棉铃虫和家蝇羧酸酯酶以及GSH—S—转移酶的诱导作用

高希武 梁同庭   

  • 出版日期:1993-05-20 发布日期:1993-05-20

INDUCTION OF CARBOXYLESTERASE AND GLUTATHIONE-S-TRANS-FERASE ACTIVITIES BY ATRAZINE AND DICHLORVOS INLARVAE OF HELIOTHIS ARMIGERA AND MUSCADOMESTICA VICINA

GAO XI-WU LIANG TONG-TING   

  • Online:1993-05-20 Published:1993-05-20

摘要: 在一定时间内,刚特拉津 (Atrazinc)对棉铃虫 Heliothis armigera 幼虫羧酸酯酶以及GSH-S-转移酶(GST)活性有明显的诱导作用,羧酸酯酶活性最高增加146%,GST增加280%。 对羧酸酯酶的诱导高峰时间要落后于GST,不同施药剂量的诱导高峰时间以及导增加的量也不相同。 敌敌畏对家蝇Musca domestica vicina GST活性没有明显的诱导作用,阿特拉津对家蝇GST活性也没有产生诱导作用。

关键词: 棉铃虫, 家蝇, 羧酸酯酶, GSH—S—转移酶, 诱导作用

Abstract: Dilute solutions of atrazine and dichlorvos were used to treat the larvae of cotton boll worm Heliothis armigera and housefly Musca domestica vicina by topic application or mixing them with the diets for rearing the larvae. The activities of carboxylesterase (CarE) and glutatione-S-transferase (CST), using the supernatant protions of the homogenates of the larvae,were determined according to the methods of Asperen (1962) and Habig ct al (1981) respec tively. The results showed that atrazine induced Gate and GST activities significantly in the cotton bollworm to about 146% and 280% respectively. The peak of Care activity induced byatrazine appeared earlier than that of GST. Both the time and magnitude of Care or GST activity varied with the amount of atrzine used in the topic application. In the larvae of house fly, dichlorvos was very poor inducer of Care or GST activity and atrazine could not increase GST activity.

Key words: Heliothis armigera——Murca domestica vicina——carboxylesterase—— glutathione-S-transferase——enzymic induction