›› 1994, Vol. 37 ›› Issue (2): 159-164.

• 研究论文 • 上一篇    下一篇

意蜂三个品系间的基因差异

关迎辉 李绍文 李举怀 陈盛禄   

  • 出版日期:1994-05-20 发布日期:1994-05-20

GENOVARIATION IN THREE RACES OF APISMELLIFERA LIGUSTICA

GUAN YING-HUI LI SHAO-WIEN LI JU-HUAI CHEN SHENG-LU   

  • Online:1994-05-20 Published:1994-05-20

摘要: 用聚丙烯酰胺凝胶等电聚焦(1EF)电泳对意蜂(Apis mellifera ligustica)三个品系(渐农大A系, 湖北意蜂和引进的原意蜂)的成年工蜂进行了苹果酸脱氢酶 (MDH) 同工酶的测定。它们的MDH图谱均有三个区带(MDHI、MDHll和MDHIll),其中MDHII是由三个等位基因(a、b、c) 编码的,应有六种基因型(a/a、b/b、c/c、a/b、b/c和a/c),但本实验室测定的360余头个体中,均宋 出现b/b型.浙农大A系出现了除b/b型以外的其它五种基因型,而其它两个晶系都仅出现了c/c, a/c和b/c等三种基因型。对结果进行独立性检验,浙农大A系的MDHII的基因型频率、基因频率以及 杂合度等与其它两个晶系均有很显著的差异,这说明它与这两个晶系已有了相当程度的遗传分化,因此,这个结果可以为浙农大A系的建立提供一个遗传和生化的依据。

关键词: 意蜂, 苹果酸脱氢酶, 等位基因

Abstract: The malate dehydrogenase (MDH) isozymes in three races of Apis rnelliferaligustica——Zheiiang Agricultural University A (A), Hubei A. m. ligustica (B) and A. m. ligustica introduced from Italy ? were analyzed with polyacrylamide gel isoelectrofocusing (IEF). There were three zones (MDH I, MDH II and MDH Iil) in these electromorphs. MDH II was coded by three alleles: a, b and c. About 120 worker bees of each racewere analyzed. Theoretically, there should be six genotypesin MDH Il (a/a, b/b, c/c, a/b, b/c and a/c), but b/b type was not found in the experirncnt. The other five genotypes appeared in A, and only three (a/c, b/c and c/c) were found in B and C. Statistical analysis showed that there were significant differences in the genotype frequency, the allele frequency and the heterozygous degree between A and the other two races. This result provided a genetical and biochemical evidence for the establishment of race A.

Key words: Apis mellitera ligustica——malate dehydrogenase——allozymes