关键词: 亚洲玉米螟, 免疫细胞, 细胞粘附因子, 细胞免疫反应

Abstract: The larva of Asian corn borer Ostrinia furnacalis was used as the experimental insect. Stable and relatively pure haemocyte population was obtained from density gradients of 53% percoll, and two main types of immunocytes, granulocytes (GR) and plasmatocyte (PL), were successfully isolated. It was found that a haemocyticlysate had cell adhesion activity after it was activated by the beta-l, 3-glucan laminarin, or by preparing the lysate in 5 mmol Ca ion. It could be detected that isolated PL attached and spread in vitro on coverslips coated with the lysate. The factor responsible for cell adhesion was isolated from an active haemocytic lysate and purified by ammonium sulfate precipitation, carboxymethyl-cellulose ion-exchange chromalography and ConA-Sepharose 4B affinity chromatography. The cell adhesion factor had a molecular weight of about 64KD in apolyacrylamide gel concentration gradient electrophoresis. It was found that the purified cell adhesion factor was only present in GR. A protein having a molecular weight of about 390 KD was purified from plasma with the same methods. It is possibly a plasma coagulogen.

Key words: Ostrinia fumacalis, immunocyte, cell adhesion factor, cellular immune re- sponse