关键词: 粘虫, α-淀粉酶, 酶活性, pH值, 氯离子, 噁唑哒嗪

Abstract:

The effects of different enzymatic buffers, pH value, chloridion, and oxadiazolyl pyridazinone on the midgut α-amylase activity in 5th-instar 2nd-day larvae of Pseudaletia separata Walker were assayed. The results showed that sodium acetate buffer (pH 5.8) and phosphate buffer (pH 8.0) were most beneficial to α-amylase activity in their buffer series with different pH value, with the maximal activity as high as 4.49 and 4.97 U respectively. The activities against 5, 10, 20, 40, 80 mmol/L chloridion showed first decreasing and then increasing in sodium acetate buffer (pH 5.8), whereas they showed only gradually decreasing in phosphate buffer (pH 8.0). Inhibition of 1.4 mmol/L oxadiazolyl pyridazinone against α-amylase activity was as high as 70 percent, and thereafter gradually decreased with the increase of α-amylase protein.

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Key words: Pseudaletia separata, α-amylase, enzyme activity, pH value, chloridion, oxadiazolyl pyridazinone