›› 2006, Vol. 49 ›› Issue (2): 230-234.

• 研究论文 • 上一篇    下一篇

菜青虫感染玫烟色拟青霉后血淋巴蛋白质含量及几种保护酶活力的变化

张仙红, 王宏民, 李文英, 贺运春, 郝赤   

  1. 山西农业大学农学院,山西太谷030801
  • 出版日期:2006-05-15 发布日期:2006-11-20
  • 通讯作者: 郝赤

Changes in the protein content in haemolymph and activities of protective enzymes of Pieris rapae infected by Paecilomyces fumosoroseus(Deuteromycotina: Hyphomycetes)

ZHANG Xian-Hong, WANG Hong-Min, LI Wen-Ying, HE Yun-Chun, HAO Chi   

  • Online:2006-05-15 Published:2006-11-20

摘要: 对玫烟色拟青霉Paecilomyces fumosoroseus侵染3、4龄菜青虫Pieris rapae后,其血淋巴中蛋白质含量、体内保护酶及谷胱甘肽S-转移酶活力进行了研究。结果表明,感病的3、4龄菜青虫血淋巴中蛋白质含量明显低于同期未感染的幼虫;感病虫体内超氧化物歧化酶、过氧化氢酶和过氧化物酶的活力发生了不同程度的改变,其中对3龄菜青虫体内酶活力的影响比4龄幼虫大。此外被侵染的3、4龄菜青虫体内谷胱甘肽S-转移酶活力在感病前期显著高于同期未感染的菜青虫,而在感病后期明显低于同期未感染的菜青虫。

关键词: 菜青虫, 玫烟色拟青霉, 病理, 蛋白质含量, 保护酶, 谷胱甘肽S转移酶

Abstract:

Changes in the protein content in haemolymph and activities of protective enzymes of Pieris rapae infected by Paecilomyces fumosoroseus were assayed. The results indicated that  the protein content in haemolymph of the 3rd and 4th instar larvae of the P. rapae infected by P. fumosoroseus decreased obviously. The SOD, POD and CAT activity of the infected P. rapae was significantly lower than that of the control not infected. The effect on the 3rd instar larvae of P. rapae was more obvious than on the 4th instar. In addition, the GSTs activity of the 3rd and 4th instar larvae of P. rapae was higher in the early period of infection, but was lower in the late period of infection than that of the healthy P. rapae in the corresponding period.


Key words: Pieris rapae, Paecilomyces fumosoroseus, pathogenesis, protein content, protective enzymes, GSTs