›› 2006, Vol. 49 ›› Issue (2): 241-246.

• 研究论文 • 上一篇    下一篇

印楝种子提取物对荔枝蝽的毒性及与其等位酶基因型之间的关系

卢芙萍,赵冬香,刘业平,王爱萍,陈青   

  1. 中国热带农业科学院环境与植物保护研究所,海南儋州571737
  • 出版日期:2006-05-16 发布日期:2006-11-20
  • 通讯作者: 赵冬香

Toxicity of neem seed extract to Tessaratoma papillosa (Drury) relative to its allozyme genotypes

LU Fu-Ping, ZHAO Dong-Xiang, LIU Ye-Ping, WANG Ai-Ping, CHEN Qing   

  • Online:2006-05-16 Published:2006-11-20

摘要: 用5.2 mg/mL(LC50)的印楝种子提取物对荔枝蝽1龄若虫进行急性毒性处理,24 h死亡率为51.8%。通过等位酶分析检测了死亡与存活试虫两种酶(PGI和MDH),两个基因座(PgiMdh)上各基因型及等位基因与印楝种子提取物毒性之间的关系,进行致死性差异比较研究。结果表明,印楝种子提取物对具有不同基因型及等位基因个体的致死性存在差异。在Pgi基因座上,Pgi-bb基因型死亡率最高,为84%,Pgi-aaPgi-cc基因型死亡率较低,分别为0和7%,且与死亡率最高的Pgi-bb基因型存在显著差异(P<0.05)。在基因座Mdh上,Mdh-aa基因型个体死亡率最高(93%),而具有Mdh-cc基因型的个体全部存活了下来, 另外3个基因型Mdh-abMdh-bbMdh-bc死亡率居中,都与Mdh-aa、Mdh-cc基因型死亡率之间存在显著差异。在等位基因上,Pgi-aMdh-c个体的死亡率都最低,与各自其他两个等位基因的死亡率之间存在显著差异。结果说明不同基因型个体对印楝提取物具有不同的反应,印楝种子提取物对荔枝蝽等位酶基因型及等位基因存在选择性致死作用。这种荔枝蝽对印楝种子提取物的敏感性与其等位酶基因型及等位基因之间显明的相关关系提示我们,可将荔枝蝽种群中对印楝种子提取物敏感性低的基因型及等位基因作为遗传标记去监测荔枝蝽对印楝种子提取物的抗性状况。

关键词: 荔枝蝽, 印楝种子提取物, 等位酶, 基因型, 等位基因, 选择性致死作用

Abstract:

The relationships between the susceptibility to neem seed extract and the allozyme genotypes were examined in the first instar nymphs of Tessaratoma papillosa (Drury) for two polymorphic enzyme loci of Pgi and Mdh using allozyme analysis. Acute exposures of the insect to 5.2 mg/mL (LC50 value) neem seed extract resulted in 51.8% mortality in 24 h. Under the given experimental conditions, insect mortalities were significantly different among certain genotypes and alleles. At locus Pgi, the insects with the Pgi-bb genotype displayed the highest mortality (84%), whereas those with Pgi-aa and Pgi-cc showed the lowest mortalities (0 and 7%, respectively), which were significantly different from that of Pgi-bb. At locus Mdh, the insects with the genotype Mdh-cc and Mdh-aa exhibited the highest mortality (93%), but no mortality was observed in the insects with the genotype Mdh-cc. These results clearly indicated that the insects with genotype
Mdh-aa and Mdh-cc were significantly different from those with other three genotypes Mdh-ab, Mdh-bb and Mdh-bc in response to neem seed extract. In contrast, the mortalities of the insects with the Pgi-a and Mdh-c allele were the lowest, and were significantly different from those with other alleles. Our studies showed that individuals of T. papillosa with different genotypes had significantly different responses to neem seed extract. Such distinct relationships between the insect susceptibility to neem seed extract and its allozyme genotypes may allow us to use certain genotypes and alleles as genetic markers to assess the susceptibility of T. papillosa to neem seed extract.


Key words: Tessaratoma papillosa, neem seed extract, allozymes, genotype, allele, selective lethal effect