›› 2006, Vol. 49 ›› Issue (4): 543-549.

• 研究论文 •    下一篇

家蚕保存系统红色卵的遗传分析

范晓东,代方银*,童晓玲,鲁成,向仲怀   

  1. (西南大学蚕学与生物技术学院,农业部蚕学重点实验室),重庆400716
  • 出版日期:2006-09-26 发布日期:2006-08-20
  • 通讯作者: 代方银

Inheritance analysis of red color egg systems preserved in silkworm gene bank in China

FAN Xiao-Dong,DAI Fang-Yin,TONG Xiao-Ling,LU Cheng,XIANG Zhong-Huai   

  1. Key Laboratory of Sericulture of Agricultural Ministry, College of Sericulture and Biotechnology, Southwest University, Chongqing 400716, China
  • Online:2006-09-26 Published:2006-08-20
  • Contact: DAI Fang-Yin

摘要:

用遗传背景清楚的家蚕Bombyx mori红卵(re)、白卵(w-2、pe)、第4褐卵(b-4)的标志基因系统和正常型黑卵系统与我国家蚕基因库保存的20个红色卵系统杂交,进行顺反测验,分析了它们的卵色支配基因及遗传规律。结果发现:①在03-310系统中存在家蚕卵色新突变pink egg,与红卵re 等位,基因符号为rep,表型特征为:卵淡红色,成虫蛾眼也为淡红色;②6个系统为红卵(re)的纯合系统,还有5个系统除具有rere基因型外,还具有支配白色卵或浅红色或橙红色卵的突变基因;③2个系统为第4褐卵(b4)的纯合系统; ④6个系统的红褐色卵为母性影响遗传;⑤发现家蚕卵色基因b-4和r-e的互补关系,b-4/b-4 re/re基因型表现为新的卵色——橙黄色。
 

关键词: 家蚕, 红色卵, 遗传分析, 顺反测验, 基因互作

Abstract:

The known marker systems of red egg ( re ), white egg (w2pe), the fourth brown egg ( b-4 ) and the normal type-black egg of silkworm Bombyx mori were crossed with 20 red egg systems of unknown genes preserved in silkworm gene bank in China, and cis-trans complementary test was conducted to identify the genes controlling their egg color and to study their inheritance. A new mutant gene, pink like egg, an allele of re, designated as rep, was isolated from 03-310 system. Phenotypically, this mutant was found to be characterized by pink eggs and pink eyes of the moth. Six systems appeared to be homozygous for the genotype of re/re. Five systems had additional genes controlling the white egg, light red egg or orange red egg besides the genotype of re/re. Two systems were confirmed to be homozygotes of the fourth brown egg (b-4). Redbrown egg occurred in the other 6 systems and was controlled by maternal inheritance. The two egg color controlling genes b-4 and re were found to be complementary, and the homozygote (b-4/b-4 re/re), a new egg color mutant, appeared as orange egg. 13 Refs. In Chinese.

Key words: Bombyx mori, red color egg, inheritance analysis, cis-trans test, complementary genes