麦长管蚜唾液中几种酶的鉴定、活力测定与功能分析

›› 2006, Vol. 49 ›› Issue (5): 768-774.

麦长管蚜唾液中几种酶的鉴定、活力测定与功能分析

郭光喜，刘勇^*，杨景娟，马向真

(山东农业大学植物保护学院，山东泰安271018)

出版日期:2006-11-06 发布日期:2006-10-20
通讯作者: 刘勇

Identification, activity and function determination of several salivary enzymes secreted by Macrosiphum avenae

GUO Guang-Xi, LIU Yong, YANG Jing-Juan, MA Xiang-Zhen

(College of Plant Protection, Shandong Agricultural University, Tai'an, Shandong 271018, China)

Online:2006-11-06 Published:2006-10-20
Contact: LIU Yong

摘要/Abstract

摘要：

用Parafilm膜夹营养液法，以两种食料介质饲喂麦长管蚜Macrosiphum avenae 3龄若蚜并收集其唾液，对唾液中的酶类进行了鉴定、活力测定和功能分析。结果表明，在20%蔗糖介质提取液中，鉴定有果胶酶、多酚氧化酶和纤维素酶；在水介质提取液中鉴定有纤维素酶；两种介质提取液中都未鉴定出过氧化物酶。酶活力测定结果表明，在20%蔗糖介质提取液中，每30头蚜虫分泌的果胶酶、多酚氧化酶和纤维素酶的酶活力分别为2.59×10^-3 U/g、7×10^-3 U/g和7.89×10^-3 U/g；在水介质提取液中，纤维素酶活力为3.68×10^-3 U/g。行为反应试验结果表明，果胶酶处理麦苗的挥发物组分能引起麦长管蚜寄生性天敌燕麦蚜茧蜂Aphidius avenae和捕食性天敌七星瓢虫Coccinella septempunctata 的嗅觉偏好反应，因此，果胶酶在麦长管蚜取食诱导小麦植株的间接防御反应中具有重要作用。

关键词: 麦长管蚜, 燕麦蚜茧蜂, 七星瓢虫, 果胶酶, 纤维素酶, 多酚氧化酶, 酶活力, 行为反应

Abstract:

Parafilm method was adopted for feeding the third instar nymphs of Macrosiphum avenae on two liquid diets (20% sucrose and redistilled water). Their saliva after feeding for 24 h was collected and assayed identifying salivary enzymes and determining their activities. The results showed that pectinase, polyphenol oxidase and cellulase were identified in 20% sucrose diet; cellulase was identified in redistilled water diet; peroxidase was not identified in both diets. The activities of pectinase, polyphenol oxidase and cellulase secreted into 20% sucrose diet by 30 aphids were 2.59×10^-3U/g, 7×10^-3 U/g and 7.89×10^-3 U/g, respectively; and cellulase activity secreted into redistilled water diet by 30 aphids was 3.68×10^-3 U/g. In the behavioral response test, the predator Coccinella septempunctata and parasitoid Aphidius avenae of M. avenae showed much preference for the volatiles in wheat seedlings treated by pectinase. It was so concluded that pectinase secreted by M. avenae play an important role in wheat plant indirect defense induced by aphid feeding.

Key words: Macrosiphum avenae, Aphidius avenae, Coccinella septempunctata, pectinase, cellulase, polyphenol oxidase, enzyme activity, behavioral response

郭光喜，刘勇^*，杨景娟，马向真. 麦长管蚜唾液中几种酶的鉴定、活力测定与功能分析[J]. , 2006, 49(5): 768-774.

GUO Guang-Xi, LIU Yong, YANG Jing-Juan, MA Xiang-Zhen. Identification, activity and function determination of several salivary enzymes secreted by Macrosiphum avenae[J]. , 2006, 49(5): 768-774.

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