关键词: 黄粉虫, β-1, 3-葡聚糖识别蛋白, 分离纯化, 生物学功能, 酚氧化酶原激活系统, 先天性免疫

Abstract: One kind of β-1,3-glucan recognition protein (Tm-βGRP) was purified from Tenebrio molitor larvae by such traditional means as neutral salt precipitation and gel chromatography, etc., and its biological functions in pro-phenoloxidase activation system were primarily studied. The results showed that the molecular weight of Tm-βGRP was approximately 70 kDa, and the protein mainly existed in plasma. Purified Tm-βGRP could only specifically recognize β-1,3-glucan, but not peptidoglycan. During the process of pro-phenoloxidase activation induced by β-1,3-glucan, with the proceeding of pro-phenoloxidase activation, the amount of endogenous Tm-βGRP decreased significantly. The polyclonal antibody against Tm-βGRP could inhibit phenoloxidase activity in the hemolymph of T. molitor induced by β-1, 3-glucan in a dose-dependent manner. This research may help us deeply elucidate the biological function of β-1, 3-glucan in pro-phenoloxidase activation system in the hemolymph of T. molitor.

Key words: Tenebrio molitor, β-1, 3-glucan recognition protein, isolation and purification, biological function, pro-phenoloxidase activation system, innate immunity