›› 2009, Vol. 52 ›› Issue (8): 885-891.

• 研究论文 • 上一篇    下一篇

我国根瘤蚜mtDNA COⅠ遗传多样性与系统发育

孙庆华,陈迎春,王海波,D.A. DOWNIE,翟衡   

  • 出版日期:2009-10-07 发布日期:2009-08-20
  • 通讯作者: 翟衡

Origin and genetic diversity of grape phylloxera in China


  • Online:2009-10-07 Published:2009-08-20

摘要: 通过线粒体DNA COⅠ多态性研究了我国根瘤蚜Daktulosphaira vitifoliae Fitch的遗传分化与系统发育,并将我国根瘤蚜单倍型与GenBank中已发表的85个单倍型进行了聚类分析。结果表明:序列中A,C,T,G 4种核苷酸的比例分别为34.8%,15.8%,39.2%和10.2%, 29 个变异位点中单一多态位点 12 个,简约信息位点17 个。确定了 13 种单倍型,检测5种单倍型,其中上海群体单倍型相对丰富,且上海葡萄根瘤蚜群体与其他3个群体之间没有共享单倍型。同时上海群体与其他3个群体的遗传距离最大(0.039~0.040),Nm最小(0.02),在分子系统发育树和单倍型网络图上为独立分支,说明我国根瘤蚜至少有两个独立的起源。

关键词: 葡萄根瘤蚜, mtDNA COⅠ, 单倍型, 遗传多样性, 系统发育

Abstract: In order to investigate levels of genetic variation and differentiation of grape phylloxera, Daktulosphaira vitifoliae Fitch in China, we sequenced the mitochondrial DNA (mtDNA) gene cytochrome oxidase Ⅰ (COⅠ) of 35 samples from four populations. Of the total analyzed sites, 29 (6.13%) were polymorphic, including 17 (3.59%) parsimony informative and 12 (2.54%) singleton sites. Nucleotide frequencies of A, C, G and T were 34.8%, 15.8%, 10.2%, and 39.2%, respectively. In total, 13 haplotypes were identified in the target region. The most common haplotypes were H3 (HAP-A) shared by 5 samples from SHJ, and H13 (HAP-C) found in 19 samples from SXX, HNH and LNX populations. The SHJ population was unique and shared no haplotypes with the other three populations. Nm ranged from 0.02 to 4.03 and the genetic distance varied from 0.001 to 0.040 between populations. Nm (0.02) was smaller, and the genetic distance (0.039-0.040) larger between SHJ and other three populations. Phylogenetic analysis and haplotype network showed that all haplotypes from SHJ formed one cluster, and the other haplotypes from SXX, HNH and LNX are grouped into another, suggesting that there were at least two introductions of grape phylloxera into China.

Key words: grape phylloxera, mtDNA COⅠ, haplotype, genetic diversity, phylogeny