关键词: 湘西黑藤, 小菜蛾, 拒食化合物, 分离, 鉴定, 拒食活性, 叶碟法

Abstract:  In order to determine the active compounds from Derris cavaleriei vines and their antifeedant activities against Plutella xylostella larvae, the active compounds were isolated by activity-guided fractionation with column chromatography and identified on the basis of MS and NMR data, and the antifeedant activity was determined by sandwich method. The results included that four compounds i.e. isolonchcarpin, magnificol, ovaliflavanone and millettocalyxin C had been isolated from the plant vines for the first time, and isolonchcarpin, magnificol and millettocalyxin C exhibited antifeeding activity, with the AFC₅₀ values of 25.5, 92.8 and 115.9 mg/L against the 3rd instar larvae of P. xylostella at 48 h after treatment, respectively, suggesting that the three compounds are antifeedant compounds against P. xylostella derived from the plant vines. The elucidation of these chemicals is important not only for understanding the insectplant relationships, but also for their potential in P. xylostella control.

Key words: Derris cavaleriei, Plutella xylostella, antifeedant compounds, isolation, identification, antifeedant activity, sandwich method