关键词: 棕尾别麻蝇, 胚胎细胞系, 核型, 同工酶, RAPD分析

Abstract: Dipteran cell lines are widely used for studies of genetics, molecular biology, developmental biology, the host-parasite relationship in insect-borne pathogenic microbes and insect antimicrobial peptides. A new cell line from Sarcophaga peregrina, designated as Sp-E-HNU11, has been established. The primary culture from minced embryos of S. peregrina was initiated on November 17, 2008, grown in Shields & Sang M3 insect cell medium at 28℃, and was split into two 26 days later. Since then, it has been subcultured for 72 passages. The cells, mainly round or spindle-shaped, adhere tightly to the flask. The population doubling time was 42 h. Most cells in metaphase observed were sub-diploid and contained ten or twelve chromosomes, which were short pole-like except two micro chromosomes. β-naphthyl esterase and aspartate aminotransferase isozymes of this cell lines displayed one and three bands, respectively, in sodium dodecyl sulfate polyacrylamide gel electrophoresis. In random amplified polymorphic DNA analysis, the Sp-E-HNU11 cells had a banding pattern markedly different from the ones of Px-E-HNU12, IPLB-Sf-9 and Bm-21E-HNU5 cells. The establishment of the new cell line would provide an additional tool and vector for research in insect antimicrobial peptides and related fields.

Key words: Sarcophaga peregrina, embryos cell line, karyogram, isozyme, RAPD analysis