关键词:  , 金特异结合肽； Bac-to-Bac； 融合表达； 表面展示； 重组杆状病毒； 纳米复合体

Abstract: The application of nanogold in bioprobe technology is a major research field, which mainly depends on the effective binding of biomolecules with gold nanoparticles. Using the strategy of gene recombination, we studied the feasibility of combining baculoviral surface display with peptide-mediated immobilization to make bio-inorganic composites. A gene encoding an Auspecific binding peptide (Aubp) was synthesized and fused to the N-terminus of the gp64 derived from BmNPV to construct the recombinant plasmid pFB-gp64-Au, which was then transposed to shuttle vector Bacmid by Bac-to-Bac system. The recombinant AcMNPV baculovirus was harvested after transfection of the recombinant Bacmid into the Sf9 insect cells and its binding with colloid gold prepared by NaHB reduction method was primarily studied. The results showed that recombinant Bacmid was successfully constructed and envelope-modified baculovirus with gold-specific binding peptide was harvested. Au-bp mediated virus-nanoparticle complex was visualized by transmission electron microscopy (TEM) analysis. The study will be helpful to the research of fabricating bio-inorganic compound system and the application of nano materials in biological field.

Key words: Au-binding peptide, Bac-to-Bac, fusion expression, surface display, recombinant baculovirus, nanocomposite