›› 2011, Vol. 54 ›› Issue (8): 933-937.doi:

• 综述 • 上一篇    下一篇

蜕皮激素与其受体EcR-USP的转录调控机制

 李康, 李胜, 曹阳   

  • 收稿日期:2011-03-01 出版日期:2011-08-20 发布日期:2011-08-20
  • 通讯作者: 李胜 E-mail: shengli@sippe.ac.cn
  • 作者简介:李康, 男, 1985 年生, 硕士研究生, 主要从事昆虫生理生化和家蚕遗传研究, likang0118@yahoo.com.cn
  • 基金资助:

     国家自然科学基金项目(30870299, 30870335); 上海市重点项目(10JC1416700); 中国科学院“百人计划”择优支持项目; 中国科学院知识创新工程项目(KSCX-EW-J-12)

Transcriptional regulation by 20-hydroxyecydsone and its nuclear receptor EcR-USP

 LI  Kang, LI  Sheng, CAO  Yang   

  • Received:2011-03-01 Online:2011-08-20 Published:2011-08-20
  • Contact: LI Sheng E-mail: shengli@sippe.ac.cn
  • About author: likang0118@yahoo.com.cn

摘要: 蜕皮激素20-羟基蜕皮酮(20-hydroxyecdysone, 20E)是一种典型的类固醇激素, 主导调控昆虫的蜕皮、变态、生殖等重要生理过程。20E受体EcR-USP已被鉴定近20年, 20E与其受体复合物的转录调控机制也有了许多重要突破。已有研究表明: (1)20E受体由核受体EcR和USP形成; (2)EcR-USP异源二聚体在分子伴侣蛋白复合物的协助下获得DNA结合活性; (3)20E通过解除共阻遏因子和募集共激活因子来激活EcRUSP异源二聚体并启动下游基因的转录; (4)20E-EcR-USP配体-受体复合物引发20E初级应答基因的表达, 由20E初级反应基因编码的转录因子诱导表达的20E次级应答基因级联放大20E信号, 从而调控昆虫蜕皮、变态、生殖等生理过程。

关键词: 蜕皮激素, 20-羟基蜕皮酮(20E), EcR-USP, 转录调控, 分子蛋白伴侣蛋白复合物, 共阻遏因子, 共激活因子, 细胞程序化死亡

Abstract: The molting hormone, 20-hydroxyecdysone (20E), is an insect steroid hormone which plays a dominant role in the regulation of insect molting, metamorphosis, reproduction and other physiological processes. The 20E nuclear receptor, EcR-USP, has been identified for 20 years, and several breakthroughs have been recently made in understanding the transcriptional regulation mechanisms of 20E-cR-USP. Recent research indicates that: (1) the 20E receptor is composed of two nuclear receptor molecules, i. e., EcR and USP. (2) The EcR-USP heterodimer obtains DNA binding activity with the assistance of a molecular chaperone-containing heterocomplex. (3) 20E transcriptionally activates the EcR-USP heterodimer by releasing corepressors and recruiting co-activators. (4) The ligand-receptor complex, 20E-EcR-USP, triggers a transcriptional cascade, including transcription of the 20E primaryresponse genes and the 20E secondaryresponse genes induced by transcription factors encoded by the 20E primary-response genes, to regulate physiological and developmental events.

Key words: Ecdysone, 20-hydrxoecdysone(20E), EcR-USP, transcriptional regulation; MCH, co-repressor, co-activator, PCD