›› 2011, Vol. 54 ›› Issue (9): 1027-1033.doi:

• 研究论文 • 上一篇    下一篇

抗吡虫啉棉蚜对其他新烟碱类药剂的交互抗性及相关酶的活性变化

 史晓斌, 石绪根, 王红艳, 夏晓明, 王开运   

  • 收稿日期:2011-01-06 出版日期:2011-09-20 发布日期:2011-09-20
  • 通讯作者: 王开运 E-mail:kywang@sdau.edu.cn
  • 作者简介:史晓斌,女,1986年生,山东烟台人,硕士研究生,研究方向为农药毒理与有害生物抗药性,E-mail: wky@sdau.edu.cn
  • 基金资助:
    国家公益性行业(农业)科研专项(200903033)

Cross-resistance of the imidacloprid-resistant population of Aphis gossypii Glover (Homoptera: Aphididae) to other neonicotinoid insecticides and changes in activities of related enzymes

 SHI  Xiao-Bin, SHI  Xu-Gen, WANG  Hong-Yan, XIA  Xiao-Ming, WANG  Kai-Yun   

  • Received:2011-01-06 Online:2011-09-20 Published:2011-09-20
  • Contact: WANG Kai-Yun E-mail:kywang@sdau.edu.cn
  • About author:E-mail: wky@sdau.edu.cn

摘要: 为明确抗吡虫啉棉蚜Aphis gossypii对其他新烟碱药剂交互抗性及相关酶活性的变化, 本研究以室内筛选的棉蚜吡虫啉抗性种群、山东夏津县田间多抗种群和敏感种群为材料,采用生测法测定了这3种不同抗性种群棉蚜对呋虫胺和烯啶虫胺的交互抗性,以及3种解毒酶抑制剂对吡虫啉等药剂的增效作用,采用生化分析法测定了这3个种群棉蚜解毒酶及乙酰胆碱酯酶的活性和药剂的抑制作用。结果表明:吡虫啉抗性种群、夏津田间多抗种群的棉蚜对呋虫胺均无交互抗性,但对烯啶虫胺的交互抗性分别达5.28和4.89倍。呋虫胺对抗吡虫啉棉蚜的羧酸酯酶、乙酰胆碱酯酶及谷胱甘肽-S-转移酶都有显著的抑制作用;烯啶虫胺对抗吡虫啉棉蚜的羧酸酯酶、乙酰胆碱酯酶及谷胱甘肽-S-转移酶的抑制作用较小。羧酸酯酶抑制剂TPP和多功能氧化酶抑制剂PBO对吡虫啉和烯啶虫胺都有明显的增效作用,但对呋虫胺的增效作用不明显; 谷胱甘肽-S-转移酶抑制剂DEM对3种药剂均没有明显的增效作用。呋虫胺和烯啶虫胺都可以抑制抗吡虫啉棉蚜的解毒酶及乙酰胆碱酯酶,其中以呋虫胺的抑制效果较为显著。结果显示呋虫胺在抗吡虫啉棉蚜治理中的应用价值较大,其结构可为今后新烟碱类药剂的开发提供参考。

关键词: 棉蚜, 吡虫啉, 呋虫胺, 烯啶虫胺, 交互抗性, 抗性机理

Abstract: In order to clarify the cross-resistance and the change in related enzymes of the imidacloprid-resistant population of Aphis gossypii to other neonicotinoid insecticides, the bioassay method was used to determine the cross-resistance of different resistant populations of imidacloprid-resistant A. gossypii to dinotefuran and nitenpyram, and the synergism of three detoxification enzyme inhibitors to imidacloprid and other two neonicotinoid insecticides using cotton aphids of the imidacloprid-resistant population selected in the laboratory, the Xiajin resistant population in the field in Shandong and the susceptible population. The activities of detoxification enzymes and AChE of three populations of cotton aphid and the inhibition effect of insecticides were determined through biochemical analysis. The results showed that the imidacloprid-resistant population and the Xiajin resistant population exhibited no cross-resistance to dinotefuran, but showed 5.28-fold and 4.89-fold cross-resistance to nitenpyram, respectively. Dinotefuran could significantly inhibit the activities of CarE, GST and AChE of the imidacloprid-resistant cotton aphid. Nitenpyram showed little effect on the activities of CarE, GST and AChE of the imidacloprid-resistant cotton aphid. Carboxylesterase inhibitor TPP and mixed-functional oxidase inhibitor PBO had obvious synergism to imidacloprid and nitenpyram, while had little synergism to dinotefuran. Glutathione-S-transferase inhibitor DEM showed no obvious synergism to the three insecticides. Dinotefuran and nitenpyram could inhibit the activities of detoxification enzymes and AChE of the imidacloprid-resistant conton aphid, with dinotefuran showing significant effect. The results demonstrate the great application value of dinotefuran in control of the imidacloprid-resistant cotton aphid, and its structure can provide a reference to the development of neonicotinoid insecticides in the future.

Key words: Aphis gossypii, imidacloprid, dinotefuran, nitenpyram, cross-resistance, resistant mechanism