›› 2011, Vol. 54 ›› Issue (9): 1042-1050.doi:

• 研究论文 • 上一篇    下一篇

美洲黑杨不同无性系对分月扇舟蛾幼虫的抗性及相关机理

方杰,赵博光   

  • 收稿日期:2010-12-31 出版日期:2011-09-20 发布日期:2011-09-20
  • 通讯作者: 赵博光 E-mail:zhbg596@126.com
  • 作者简介:方杰,男,1979年生,安徽祁门人,硕士,讲师,研究方向为昆虫化学生态学, E-mail: ahufangjie@126.com
  • 基金资助:
    国家林业局“948”项目(2005-4-19)

Resistance of different clones of Populus deltoides to Clostera anastomosis (Lepidoptera: Notodontidae) larvae and the related mechanisms

 FANG  Jie, ZHAO  Bo-Guang   

  • Received:2010-12-31 Online:2011-09-20 Published:2011-09-20
  • Contact: ZHAO Bo-Guang E-mail:zhbg596@126.com
  • About author:E-mail: ahufangjie@126.com

摘要: 为了选育出在生产上具有推广价值的美洲黑杨Populus deltoides优良抗虫品系,本研究以分月扇舟蛾Clostera anastomosis 3龄幼虫为材料,以I-72杨为对照评价和分析了18个美洲黑杨无性系在实验室条件(28±1℃,RH 70%,16L∶8D)下的抗虫性,通过选择性和非选择性取食实验筛选出了11个具有较强抗性的无性系; 测定了美洲黑杨主要营养物质和次生代谢物质含量,研究了不同无性系对分月扇舟蛾体内保护酶和消化酶活性及对该虫生长发育和食物利用的影响。结果表明:美洲黑杨不同无性系抗性的大小与它们叶片中总酚含量呈明显正相关趋势; 抗性水平不同的无性系对分月扇舟蛾幼虫的生长发育和食物利用有不同的影响,表现在幼虫体重、每日体重增加量、相对生长率、近似消化率、食物利用率和食物转化率等主要生长发育指标的变化上,同时对分月扇舟蛾幼虫取食量和排粪量亦有不同的影响。结果说明,分月扇舟蛾对次生代谢物质的反应更为敏感,抗性无性系对分月扇舟蛾幼虫体内SOD和CAT酶活性有明显的激活作用。

关键词: 美洲黑杨, 分月扇舟蛾, 选择试验, 抗性, 无性系, 食物利用

Abstract: In order to provide scientific suggestions for selecting new poplar strains resistant to insects, the resistance of 18 clones of Populus deltoids to 3rd instar larva of Clostera anastomosis was evaluated and analyzed in laboratory conditions (28±1℃, RH 70%, 16L∶8D). The choice and no-choice experiments indicated that eleven of 18 clones had higher resistance to C. anastomosis larvae than I-72 (control). Main nutrients and secondary metabolites were measured, and activities of digestive and protective enzymes and their effects on food utilization and insect development were also studied. The results showed resistance of the clones tested in P. deltoides to C. anastomosis larvae was statistically and positively related with the content of phenolic compounds of their leaves. Differential effects of clones of P. deltoides on the growth and food utilization existed in larvae of C. anastomosis, as showed in the changes of the main indicators of growth and food utilization, such as larval weight, daily body weight gain, relative growth rate, approximate digestibility, efficiency of conversion of ingested food, and efficiency of conversion of digested food. Differential effects of clones of P. deltoides on food intake and feces excretion in larvae of C. anastomosis also existed. The results suggest that C. anastomosis are more sensitive to secondary metabolites and the clones of P.deltoides have apparently active effects on activities of SOD and CAT in C. anastomosis larvae.

Key words: Populus deltoides, Clostera anastomosis, choice test, resistance, clone, food utilization