关键词: 黄曲条跳甲, Solexa测序, 序列重叠群, 独立基因, 转录组

Abstract: The striped flea beetle, Phyllotreta striolata (Fabricius), is an important pest damaging cruciferous vegetables. In order to investigate the profile of gene expression and elucidate the functional genes, we sequenced the transcriptome of the adult of P. striolata by Illumina’s Solexa sequencing technology, and analyzed the data of expressed sequence tags (ESTs) by using SOAPdenovo system. A total of 4 924 contigs were obtained including 2 209 unigenes of orthologous genes relating to Drosophila melanogaster and 610 species-specific unigenes of P. striolata based on Gene Ontology and KEGG databases. We found that most of unigenes contain function domains with binding capacity and catalytic activity. More than 100 unigenes are involved in gamete generation, ovarian follicle cell development and mating behavior. Three hundred sixty-three unigenes may be involved in 40 different metabolic pathways based on KEGG database. The finding that 363 unigenes are involved in regulation pathway of biological rhythm and plant secondary metabolites will be useful to clarify the mechanism of behaviors of this insect such as oviposition rhythm, etc. Moreover, the sequence resources presented in this study provide useful information to develop new strategies to manage this pest.

Key words: Phyllotreta striolata, Illumina’s Solexa sequencing technology, contig, unigene, transcriptome